Recombinant human growth hormone alters acute phase reactant proteins, cytokine expression, and liver morphology in burned rats

Marc G. Jeschke, David Herndon, Steven Wolf, Meelie A. Debroy, Jyoti Rai, Bart J. Lichtenbelt, Robert E. Barrow

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

Background. The effects of exogenous recombinant human growth hormone (rhGH) on hepatic acute phase reactant proteins, cytokine expression, and liver morphology were studied in thermally injured rats to define whether rhGH alters the acute phase response. Materials and methods. Sprague-Dawley rats (56 males) receiving a 60% TBSA third-degree scald burn were randomly divided into two groups to receive either 2.5 mg/kg/day sc rhGH or saline. Rats were sacrificed on Postburn Days 1, 2, 5, and 7. Serum acute phase reactant proteins and cytokines TNF-α, IL-1α, IL-1β, and IL-6 were measured. Hepatocyte proliferation, hepatic cytokine gene expression, and liver protein concentrations were determined. Results. Recombinant hGH increased serum albumin on Days 5 and 7 after burn (P < 0.05). Serum haptoglobin and α1-acid glycoprotein levels decreased at 2, 5, and 7 days after burn compared to saline (P < 0.05). In rats treated with rhGH, serum IL-1β decreased 1 day postburn, while serum TNF-α increased 5 days after burn compared to saline (P < 0.05). Serum IL-6 and IL-1α did not change. Hepatic RNA levels for TNF-α were significantly elevated on Day 1 postburn compared to saline (P < 0.05). Hepatic protein content increased on Days 2, 5, and 7 postburn compared to saline (P < 0.05). Hepatocyte proliferation in rhGH-treated rats increased on Day 5 after burn (P < 0.05). Conclusion. Data indicate that rhGH alters the hepatic acute phase response by decreasing type I acute phase proteins and modulating IL-1-like cytokine expression. These changes are associated with increased hepatocyte mitosis and serum and total liver protein concentrations.

Original languageEnglish (US)
Pages (from-to)122-129
Number of pages8
JournalJournal of Surgical Research
Volume83
Issue number2
DOIs
StatePublished - May 15 1999

Fingerprint

Human Growth Hormone
Acute-Phase Proteins
Growth Hormone
Cytokines
Interleukin-1
Liver
Hepatocytes
Serum
Acute-Phase Reaction
Interleukin-6
Proteins
Haptoglobins
Mitosis
Serum Albumin
Sprague Dawley Rats
Blood Proteins
Glycoproteins
RNA
Gene Expression
Acids

Keywords

  • Acute phase proteins
  • Acute phase response
  • Burns
  • Constitutive hepatic proteins
  • Cytokines
  • Rats
  • Recombinant human growth hormone

ASJC Scopus subject areas

  • Surgery

Cite this

Recombinant human growth hormone alters acute phase reactant proteins, cytokine expression, and liver morphology in burned rats. / Jeschke, Marc G.; Herndon, David; Wolf, Steven; Debroy, Meelie A.; Rai, Jyoti; Lichtenbelt, Bart J.; Barrow, Robert E.

In: Journal of Surgical Research, Vol. 83, No. 2, 15.05.1999, p. 122-129.

Research output: Contribution to journalArticle

Jeschke, Marc G. ; Herndon, David ; Wolf, Steven ; Debroy, Meelie A. ; Rai, Jyoti ; Lichtenbelt, Bart J. ; Barrow, Robert E. / Recombinant human growth hormone alters acute phase reactant proteins, cytokine expression, and liver morphology in burned rats. In: Journal of Surgical Research. 1999 ; Vol. 83, No. 2. pp. 122-129.
@article{51688190ff3149149fec77dfdc5babcb,
title = "Recombinant human growth hormone alters acute phase reactant proteins, cytokine expression, and liver morphology in burned rats",
abstract = "Background. The effects of exogenous recombinant human growth hormone (rhGH) on hepatic acute phase reactant proteins, cytokine expression, and liver morphology were studied in thermally injured rats to define whether rhGH alters the acute phase response. Materials and methods. Sprague-Dawley rats (56 males) receiving a 60{\%} TBSA third-degree scald burn were randomly divided into two groups to receive either 2.5 mg/kg/day sc rhGH or saline. Rats were sacrificed on Postburn Days 1, 2, 5, and 7. Serum acute phase reactant proteins and cytokines TNF-α, IL-1α, IL-1β, and IL-6 were measured. Hepatocyte proliferation, hepatic cytokine gene expression, and liver protein concentrations were determined. Results. Recombinant hGH increased serum albumin on Days 5 and 7 after burn (P < 0.05). Serum haptoglobin and α1-acid glycoprotein levels decreased at 2, 5, and 7 days after burn compared to saline (P < 0.05). In rats treated with rhGH, serum IL-1β decreased 1 day postburn, while serum TNF-α increased 5 days after burn compared to saline (P < 0.05). Serum IL-6 and IL-1α did not change. Hepatic RNA levels for TNF-α were significantly elevated on Day 1 postburn compared to saline (P < 0.05). Hepatic protein content increased on Days 2, 5, and 7 postburn compared to saline (P < 0.05). Hepatocyte proliferation in rhGH-treated rats increased on Day 5 after burn (P < 0.05). Conclusion. Data indicate that rhGH alters the hepatic acute phase response by decreasing type I acute phase proteins and modulating IL-1-like cytokine expression. These changes are associated with increased hepatocyte mitosis and serum and total liver protein concentrations.",
keywords = "Acute phase proteins, Acute phase response, Burns, Constitutive hepatic proteins, Cytokines, Rats, Recombinant human growth hormone",
author = "Jeschke, {Marc G.} and David Herndon and Steven Wolf and Debroy, {Meelie A.} and Jyoti Rai and Lichtenbelt, {Bart J.} and Barrow, {Robert E.}",
year = "1999",
month = "5",
day = "15",
doi = "10.1006/jsre.1999.5577",
language = "English (US)",
volume = "83",
pages = "122--129",
journal = "Journal of Surgical Research",
issn = "0022-4804",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Recombinant human growth hormone alters acute phase reactant proteins, cytokine expression, and liver morphology in burned rats

AU - Jeschke, Marc G.

AU - Herndon, David

AU - Wolf, Steven

AU - Debroy, Meelie A.

AU - Rai, Jyoti

AU - Lichtenbelt, Bart J.

AU - Barrow, Robert E.

PY - 1999/5/15

Y1 - 1999/5/15

N2 - Background. The effects of exogenous recombinant human growth hormone (rhGH) on hepatic acute phase reactant proteins, cytokine expression, and liver morphology were studied in thermally injured rats to define whether rhGH alters the acute phase response. Materials and methods. Sprague-Dawley rats (56 males) receiving a 60% TBSA third-degree scald burn were randomly divided into two groups to receive either 2.5 mg/kg/day sc rhGH or saline. Rats were sacrificed on Postburn Days 1, 2, 5, and 7. Serum acute phase reactant proteins and cytokines TNF-α, IL-1α, IL-1β, and IL-6 were measured. Hepatocyte proliferation, hepatic cytokine gene expression, and liver protein concentrations were determined. Results. Recombinant hGH increased serum albumin on Days 5 and 7 after burn (P < 0.05). Serum haptoglobin and α1-acid glycoprotein levels decreased at 2, 5, and 7 days after burn compared to saline (P < 0.05). In rats treated with rhGH, serum IL-1β decreased 1 day postburn, while serum TNF-α increased 5 days after burn compared to saline (P < 0.05). Serum IL-6 and IL-1α did not change. Hepatic RNA levels for TNF-α were significantly elevated on Day 1 postburn compared to saline (P < 0.05). Hepatic protein content increased on Days 2, 5, and 7 postburn compared to saline (P < 0.05). Hepatocyte proliferation in rhGH-treated rats increased on Day 5 after burn (P < 0.05). Conclusion. Data indicate that rhGH alters the hepatic acute phase response by decreasing type I acute phase proteins and modulating IL-1-like cytokine expression. These changes are associated with increased hepatocyte mitosis and serum and total liver protein concentrations.

AB - Background. The effects of exogenous recombinant human growth hormone (rhGH) on hepatic acute phase reactant proteins, cytokine expression, and liver morphology were studied in thermally injured rats to define whether rhGH alters the acute phase response. Materials and methods. Sprague-Dawley rats (56 males) receiving a 60% TBSA third-degree scald burn were randomly divided into two groups to receive either 2.5 mg/kg/day sc rhGH or saline. Rats were sacrificed on Postburn Days 1, 2, 5, and 7. Serum acute phase reactant proteins and cytokines TNF-α, IL-1α, IL-1β, and IL-6 were measured. Hepatocyte proliferation, hepatic cytokine gene expression, and liver protein concentrations were determined. Results. Recombinant hGH increased serum albumin on Days 5 and 7 after burn (P < 0.05). Serum haptoglobin and α1-acid glycoprotein levels decreased at 2, 5, and 7 days after burn compared to saline (P < 0.05). In rats treated with rhGH, serum IL-1β decreased 1 day postburn, while serum TNF-α increased 5 days after burn compared to saline (P < 0.05). Serum IL-6 and IL-1α did not change. Hepatic RNA levels for TNF-α were significantly elevated on Day 1 postburn compared to saline (P < 0.05). Hepatic protein content increased on Days 2, 5, and 7 postburn compared to saline (P < 0.05). Hepatocyte proliferation in rhGH-treated rats increased on Day 5 after burn (P < 0.05). Conclusion. Data indicate that rhGH alters the hepatic acute phase response by decreasing type I acute phase proteins and modulating IL-1-like cytokine expression. These changes are associated with increased hepatocyte mitosis and serum and total liver protein concentrations.

KW - Acute phase proteins

KW - Acute phase response

KW - Burns

KW - Constitutive hepatic proteins

KW - Cytokines

KW - Rats

KW - Recombinant human growth hormone

UR - http://www.scopus.com/inward/record.url?scp=0033562114&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033562114&partnerID=8YFLogxK

U2 - 10.1006/jsre.1999.5577

DO - 10.1006/jsre.1999.5577

M3 - Article

C2 - 10329105

AN - SCOPUS:0033562114

VL - 83

SP - 122

EP - 129

JO - Journal of Surgical Research

JF - Journal of Surgical Research

SN - 0022-4804

IS - 2

ER -