Recombinant human growth hormone protects thermally injured mice infected with herpes simplex virus

K. Takagi, A. Kaneko, R. E. Barrow, R. B. Pollard, Fujio Suzuki, David Herndon

Research output: Contribution to journalArticle

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Abstract

As a promoter of wound healing and skeletal muscle function, growth hormone (GH) has been used clinically in thermally injured patients. In the present study, an antiviral efficacy of GH in thermally injured mice infected with herpes simplex virus type 1 (HSV) was investigated, because the protective effect of GH in rats infected with Salmonella typhimurium has been recently reported. Recombinant GH was provided from Genentech, Inc., South San Francisco, CA. BALB/c mice were subjected to a flame burn (3rd degree, 30% of total body surface area), and designated as thermally injured mice (TI-mice). A 5LD50 dose of HSV (KOS strain) grown in Vero cells was challenged i.p. to TI-mice. IFN was measured by a plaque reduction assay and IFNγ was detected by an ELISA assay. When GH (4 mg/kg, s.c.) was administered to TI-mice every other day for 5 times beginning just after the bum, a 38% survival rate was obtained, as compared with 0% survival of TI-mice treated with saline. Protection of TI-mice infected with the same dose of HSV was also demonstrated when GH was injected to them prophylactically (1, 2 and 3 days before burn). IFN (153 IU/ml) was detected in serum specimens from mice 12 hrs after treatment with GH (10 mg/kg, s.c.), and 84 IU/ml of IFNγ was detected in these sera. These results suggest that GH administered exogenously may have a potential to protect TI-mice infected with HSV. IFN may play a role on this protective response.

Original languageEnglish (US)
JournalFASEB Journal
Volume10
Issue number6
StatePublished - 1996

Fingerprint

herpes simplex
human growth
Human Growth Hormone
Simplexvirus
Viruses
somatotropin
Growth Hormone
viruses
Human herpesvirus 1
mice
Human Herpesvirus 1
Assays
Salmonella
Vero Cells
San Francisco
Body Surface Area
assays
Salmonella typhimurium
dosage
tissue repair

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Takagi, K., Kaneko, A., Barrow, R. E., Pollard, R. B., Suzuki, F., & Herndon, D. (1996). Recombinant human growth hormone protects thermally injured mice infected with herpes simplex virus. FASEB Journal, 10(6).

Recombinant human growth hormone protects thermally injured mice infected with herpes simplex virus. / Takagi, K.; Kaneko, A.; Barrow, R. E.; Pollard, R. B.; Suzuki, Fujio; Herndon, David.

In: FASEB Journal, Vol. 10, No. 6, 1996.

Research output: Contribution to journalArticle

Takagi, K, Kaneko, A, Barrow, RE, Pollard, RB, Suzuki, F & Herndon, D 1996, 'Recombinant human growth hormone protects thermally injured mice infected with herpes simplex virus', FASEB Journal, vol. 10, no. 6.
Takagi, K. ; Kaneko, A. ; Barrow, R. E. ; Pollard, R. B. ; Suzuki, Fujio ; Herndon, David. / Recombinant human growth hormone protects thermally injured mice infected with herpes simplex virus. In: FASEB Journal. 1996 ; Vol. 10, No. 6.
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