Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes, Congrong Miao, Jennifer L. Harcourt, Joel M. Montgomery, Quynh Le Mai, Sergey A. Dryga, Kurt I. Kamrud, Bryan Rivers, Gregory J. Babcock, Jennifer Betts Oliver, James A. Comer, Mary Reynolds, Timothy M. Uyeki, Daniel Bausch, Thomas Ksiazek, William Thomas, Harold Alterson, Jonathan Smith, Donna M. Ambrosino, Larry J. Anderson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

Original languageEnglish (US)
Pages (from-to)331-333
Number of pages3
JournalClinical and Vaccine Immunology
Volume14
Issue number3
DOIs
StatePublished - Mar 2007
Externally publishedYes

Fingerprint

Coronavirus Spike Glycoproteins
Severe Acute Respiratory Syndrome
Recombinant Proteins
Assays
Coronavirus
Nucleocapsid
Antibodies
Nucleocapsid Proteins
Immunoglobulin G
Immunoassay
Kinetics
Sensitivity and Specificity
Proteins
Serum
Coronavirus nucleocapsid protein

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Immunology and Allergy
  • Microbiology (medical)

Cite this

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins. / Haynes, Lia M.; Miao, Congrong; Harcourt, Jennifer L.; Montgomery, Joel M.; Mai, Quynh Le; Dryga, Sergey A.; Kamrud, Kurt I.; Rivers, Bryan; Babcock, Gregory J.; Oliver, Jennifer Betts; Comer, James A.; Reynolds, Mary; Uyeki, Timothy M.; Bausch, Daniel; Ksiazek, Thomas; Thomas, William; Alterson, Harold; Smith, Jonathan; Ambrosino, Donna M.; Anderson, Larry J.

In: Clinical and Vaccine Immunology, Vol. 14, No. 3, 03.2007, p. 331-333.

Research output: Contribution to journalArticle

Haynes, LM, Miao, C, Harcourt, JL, Montgomery, JM, Mai, QL, Dryga, SA, Kamrud, KI, Rivers, B, Babcock, GJ, Oliver, JB, Comer, JA, Reynolds, M, Uyeki, TM, Bausch, D, Ksiazek, T, Thomas, W, Alterson, H, Smith, J, Ambrosino, DM & Anderson, LJ 2007, 'Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins', Clinical and Vaccine Immunology, vol. 14, no. 3, pp. 331-333. https://doi.org/10.1128/CVI.00351-06
Haynes, Lia M. ; Miao, Congrong ; Harcourt, Jennifer L. ; Montgomery, Joel M. ; Mai, Quynh Le ; Dryga, Sergey A. ; Kamrud, Kurt I. ; Rivers, Bryan ; Babcock, Gregory J. ; Oliver, Jennifer Betts ; Comer, James A. ; Reynolds, Mary ; Uyeki, Timothy M. ; Bausch, Daniel ; Ksiazek, Thomas ; Thomas, William ; Alterson, Harold ; Smith, Jonathan ; Ambrosino, Donna M. ; Anderson, Larry J. / Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins. In: Clinical and Vaccine Immunology. 2007 ; Vol. 14, No. 3. pp. 331-333.
@article{865026e4cfc74b469c375d72e9a53b9f,
title = "Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins",
abstract = "Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.",
author = "Haynes, {Lia M.} and Congrong Miao and Harcourt, {Jennifer L.} and Montgomery, {Joel M.} and Mai, {Quynh Le} and Dryga, {Sergey A.} and Kamrud, {Kurt I.} and Bryan Rivers and Babcock, {Gregory J.} and Oliver, {Jennifer Betts} and Comer, {James A.} and Mary Reynolds and Uyeki, {Timothy M.} and Daniel Bausch and Thomas Ksiazek and William Thomas and Harold Alterson and Jonathan Smith and Ambrosino, {Donna M.} and Anderson, {Larry J.}",
year = "2007",
month = "3",
doi = "10.1128/CVI.00351-06",
language = "English (US)",
volume = "14",
pages = "331--333",
journal = "Clinical and Vaccine Immunology",
issn = "1556-6811",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

AU - Haynes, Lia M.

AU - Miao, Congrong

AU - Harcourt, Jennifer L.

AU - Montgomery, Joel M.

AU - Mai, Quynh Le

AU - Dryga, Sergey A.

AU - Kamrud, Kurt I.

AU - Rivers, Bryan

AU - Babcock, Gregory J.

AU - Oliver, Jennifer Betts

AU - Comer, James A.

AU - Reynolds, Mary

AU - Uyeki, Timothy M.

AU - Bausch, Daniel

AU - Ksiazek, Thomas

AU - Thomas, William

AU - Alterson, Harold

AU - Smith, Jonathan

AU - Ambrosino, Donna M.

AU - Anderson, Larry J.

PY - 2007/3

Y1 - 2007/3

N2 - Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

AB - Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

UR - http://www.scopus.com/inward/record.url?scp=34147134992&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34147134992&partnerID=8YFLogxK

U2 - 10.1128/CVI.00351-06

DO - 10.1128/CVI.00351-06

M3 - Article

C2 - 17229882

AN - SCOPUS:34147134992

VL - 14

SP - 331

EP - 333

JO - Clinical and Vaccine Immunology

JF - Clinical and Vaccine Immunology

SN - 1556-6811

IS - 3

ER -