Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes, Congrong Miao, Jennifer L. Harcourt, Joel M. Montgomery, Quynh Le Mai, Sergey A. Dryga, Kurt I. Kamrud, Bryan Rivers, Gregory J. Babcock, Jennifer Betts Oliver, James A. Comer, Mary Reynolds, Timothy M. Uyeki, Daniel Bausch, Thomas Ksiazek, William Thomas, Harold Alterson, Jonathan Smith, Donna M. Ambrosino, Larry J. Anderson

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

Original languageEnglish (US)
Pages (from-to)331-333
Number of pages3
JournalClinical and Vaccine Immunology
Volume14
Issue number3
DOIs
StatePublished - Mar 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Clinical Biochemistry
  • Microbiology (medical)

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