Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes; Congrong Miao; Jennifer L. Harcourt; Joel M. Montgomery; Quynh Le Mai; Sergey A. Dryga; Kurt I. Kamrud; Bryan Rivers; Gregory J. Babcock; Jennifer Betts Oliver; James A. Comer; Mary Reynolds; Timothy M. Uyeki; Daniel Bausch; Thomas Ksiazek; William Thomas; Harold Alterson; Jonathan Smith; Donna M. Ambrosino; Larry J. Anderson

doi:10.1128/CVI.00351-06

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes
, Congrong Miao
, Jennifer L. Harcourt
, Joel M. Montgomery
, Quynh Le Mai
, Sergey A. Dryga
, Kurt I. Kamrud
, Bryan Rivers
, Gregory J. Babcock
, Jennifer Betts Oliver
, James A. Comer
, Mary Reynolds
, Timothy M. Uyeki
, Daniel Bausch
, Thomas Ksiazek
, William Thomas
, Harold Alterson
, Jonathan Smith
, Donna M. Ambrosino
, Larry J. Anderson

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

Original language	English (US)
Pages (from-to)	331-333
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	14
Issue number	3
DOIs	https://doi.org/10.1128/CVI.00351-06
State	Published - Mar 2007
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

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10.1128/CVI.00351-06

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Haynes, L. M., Miao, C., Harcourt, J. L., Montgomery, J. M., Mai, Q. L., Dryga, S. A., Kamrud, K. I., Rivers, B., Babcock, G. J., Oliver, J. B., Comer, J. A., Reynolds, M., Uyeki, T. M., Bausch, D., Ksiazek, T., Thomas, W., Alterson, H., Smith, J., Ambrosino, D. M., & Anderson, L. J. (2007). Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins. Clinical and Vaccine Immunology, 14(3), 331-333. https://doi.org/10.1128/CVI.00351-06

Haynes, LM, Miao, C, Harcourt, JL, Montgomery, JM, Mai, QL, Dryga, SA, Kamrud, KI, Rivers, B, Babcock, GJ, Oliver, JB, Comer, JA, Reynolds, M, Uyeki, TM, Bausch, D, Ksiazek, T, Thomas, W, Alterson, H, Smith, J, Ambrosino, DM & Anderson, LJ 2007, 'Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins', Clinical and Vaccine Immunology, vol. 14, no. 3, pp. 331-333. https://doi.org/10.1128/CVI.00351-06

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abstract = "Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.",

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year = "2007",

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doi = "10.1128/CVI.00351-06",

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AU - Mai, Quynh Le

AU - Dryga, Sergey A.

AU - Kamrud, Kurt I.

AU - Rivers, Bryan

AU - Babcock, Gregory J.

AU - Oliver, Jennifer Betts

AU - Comer, James A.

AU - Reynolds, Mary

AU - Uyeki, Timothy M.

AU - Bausch, Daniel

AU - Ksiazek, Thomas

AU - Thomas, William

AU - Alterson, Harold

AU - Smith, Jonathan

AU - Ambrosino, Donna M.

AU - Anderson, Larry J.

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Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Abstract

ASJC Scopus subject areas

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