Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes; Congrong Miao; Jennifer L. Harcourt; Joel M. Montgomery; Quynh Le Mai; Sergey A. Dryga; Kurt I. Kamrud; Bryan Rivers; Gregory J. Babcock; Jennifer Betts Oliver; James A. Comer; Mary Reynolds; Timothy M. Uyeki; Daniel Bausch; Thomas Ksiazek; William Thomas; Harold Alterson; Jonathan Smith; Donna M. Ambrosino; Larry J. Anderson

doi:10.1128/CVI.00351-06

Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Lia M. Haynes, Congrong Miao, Jennifer L. Harcourt, Joel M. Montgomery, Quynh Le Mai, Sergey A. Dryga, Kurt I. Kamrud, Bryan Rivers, Gregory J. Babcock, Jennifer Betts Oliver, James A. Comer, Mary Reynolds, Timothy M. Uyeki, Daniel Bausch, Thomas Ksiazek, William Thomas, Harold Alterson, Jonathan Smith, Donna M. Ambrosino, Larry J. Anderson

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.

Original language	English (US)
Pages (from-to)	331-333
Number of pages	3
Journal	Clinical and Vaccine Immunology
Volume	14
Issue number	3
DOIs	https://doi.org/10.1128/CVI.00351-06
State	Published - Mar 2007
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Clinical Biochemistry
Microbiology (medical)

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Haynes, L. M., Miao, C., Harcourt, J. L., Montgomery, J. M., Mai, Q. L., Dryga, S. A., Kamrud, K. I., Rivers, B., Babcock, G. J., Oliver, J. B., Comer, J. A., Reynolds, M., Uyeki, T. M., Bausch, D., Ksiazek, T., Thomas, W., Alterson, H., Smith, J., Ambrosino, D. M., & Anderson, L. J. (2007). Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins. Clinical and Vaccine Immunology, 14(3), 331-333. https://doi.org/10.1128/CVI.00351-06

Haynes, LM, Miao, C, Harcourt, JL, Montgomery, JM, Mai, QL, Dryga, SA, Kamrud, KI, Rivers, B, Babcock, GJ, Oliver, JB, Comer, JA, Reynolds, M, Uyeki, TM, Bausch, D, Ksiazek, T, Thomas, W, Alterson, H, Smith, J, Ambrosino, DM & Anderson, LJ 2007, 'Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins', Clinical and Vaccine Immunology, vol. 14, no. 3, pp. 331-333. https://doi.org/10.1128/CVI.00351-06

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abstract = "Recombinant severe acute respiratory syndrome (SARS) nucleocapsid and spike protein-based immunoglobulin G immunoassays were developed and evaluated. Our assays demonstrated high sensitivity and specificity to the SARS coronavirus in sera collected from patients as late as 2 years postonset of symptoms. These assays will be useful not only for routine SARS coronavirus diagnostics but also for epidemiological and antibody kinetic studies.",

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year = "2007",

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doi = "10.1128/CVI.00351-06",

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AU - Miao, Congrong

AU - Harcourt, Jennifer L.

AU - Montgomery, Joel M.

AU - Mai, Quynh Le

AU - Dryga, Sergey A.

AU - Kamrud, Kurt I.

AU - Rivers, Bryan

AU - Babcock, Gregory J.

AU - Oliver, Jennifer Betts

AU - Comer, James A.

AU - Reynolds, Mary

AU - Uyeki, Timothy M.

AU - Bausch, Daniel

AU - Ksiazek, Thomas

AU - Thomas, William

AU - Alterson, Harold

AU - Smith, Jonathan

AU - Ambrosino, Donna M.

AU - Anderson, Larry J.

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Recombinant protein-based assays for detection of antibodies to severe acute respiratory syndrome coronavirus spike and nucleocapsid proteins

Abstract

ASJC Scopus subject areas

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