Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

R. M. Kinney, J. J. Esposito, B. J B Johnson, J. T. Roehrig, J. H. Mathews, Alan Barrett, D. W. Trent

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18 Scopus citations

Abstract

cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.

Original languageEnglish (US)
Pages (from-to)3005-3013
Number of pages9
JournalJournal of General Virology
Volume69
Issue number12
StatePublished - 1988
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Kinney, R. M., Esposito, J. J., Johnson, B. J. B., Roehrig, J. T., Mathews, J. H., Barrett, A., & Trent, D. W. (1988). Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins. Journal of General Virology, 69(12), 3005-3013.