Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

R. M. Kinney; J. J. Esposito; B. J.B. Johnson; J. T. Roehrig; J. H. Mathews; A. D.T. Barrett; D. W. Trent

doi:10.1099/0022-1317-69-12-3005

Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

R. M. Kinney, J. J. Esposito, B. J.B. Johnson, J. T. Roehrig, J. H. Mathews, A. D.T. Barrett, D. W. Trent

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22 Scopus citations

Abstract

cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.

Original language	English (US)
Pages (from-to)	3005-3013
Number of pages	9
Journal	Journal of General Virology
Volume	69
Issue number	12
DOIs	https://doi.org/10.1099/0022-1317-69-12-3005
State	Published - 1988
Externally published	Yes

ASJC Scopus subject areas

Virology

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title = "Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins",

abstract = "cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.",

author = "Kinney, {R. M.} and Esposito, {J. J.} and Johnson, {B. J.B.} and Roehrig, {J. T.} and Mathews, {J. H.} and Barrett, {A. D.T.} and Trent, {D. W.}",

year = "1988",

doi = "10.1099/0022-1317-69-12-3005",

language = "English (US)",

volume = "69",

pages = "3005--3013",

journal = "Journal of General Virology",

issn = "0022-1317",

publisher = "Society for General Microbiology",

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T1 - Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

AU - Kinney, R. M.

AU - Esposito, J. J.

AU - Johnson, B. J.B.

AU - Roehrig, J. T.

AU - Mathews, J. H.

AU - Barrett, A. D.T.

AU - Trent, D. W.

PY - 1988

Y1 - 1988

N2 - cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.

AB - cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.

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Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

Abstract

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