Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins

R. M. Kinney, J. J. Esposito, B. J.B. Johnson, J. T. Roehrig, J. H. Mathews, A. D.T. Barrett, D. W. Trent

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7-5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA.

Original languageEnglish (US)
Pages (from-to)3005-3013
Number of pages9
JournalJournal of General Virology
Volume69
Issue number12
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Virology

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    Kinney, R. M., Esposito, J. J., Johnson, B. J. B., Roehrig, J. T., Mathews, J. H., Barrett, A. D. T., & Trent, D. W. (1988). Recombinant Vaccinia/Venezuelan equine encephalitis (VEE) virus express VEE structural proteins. Journal of General Virology, 69(12), 3005-3013. https://doi.org/10.1099/0022-1317-69-12-3005