Recombinase polymerase amplification-based assay to diagnose giardia in stool samples

Zachary Austin Crannell, Miguel Cabada, Alejandro Castellanos, Ayesha Irani, A. Clinton White, Rebecca Richards-Kortum

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Giardia duodenalis is one of the most commonly identified parasites in stool samples. Although relatively easy to treat, giardiasis can be difficult to detect as it presents similar to other diarrheal diseases. Here, we present a recombinase polymerase amplification-based Giardia (RPAG) assay to detect the presence of Giardia in stool samples. The RPAG assay was characterized on the bench top using stool samples spiked with Giardia cysts where it showed a limit-of-detection nearly as low as the gold standard polymerase chain reaction assay. The RPAG assay was then tested in the highlands of Peru on 104 stool samples collected from the surrounding communities where it showed 73% sensitivity and 95% specificity against a polymerase chain reaction and microscopy composite gold standard. Further improvements in clinical sensitivity will be needed for the RPAG assay to have clinical relevance.

Original languageEnglish (US)
Pages (from-to)583-587
Number of pages5
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume92
Issue number3
DOIs
StatePublished - Mar 1 2015

Fingerprint

Giardia
Recombinases
Giardiasis
Giardia lamblia
Polymerase Chain Reaction
Peru
Limit of Detection
Cysts
Microscopy
Parasites
Sensitivity and Specificity

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Recombinase polymerase amplification-based assay to diagnose giardia in stool samples. / Crannell, Zachary Austin; Cabada, Miguel; Castellanos, Alejandro; Irani, Ayesha; White, A. Clinton; Richards-Kortum, Rebecca.

In: American Journal of Tropical Medicine and Hygiene, Vol. 92, No. 3, 01.03.2015, p. 583-587.

Research output: Contribution to journalArticle

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