Recombinase polymerase amplification compared to real-time polymerase chain reaction test for the detection of fasciola hepatica in human stool

Miguel Cabada, Jose L. Malaga, Alejandro Castellanos, Kelli A. Bagwell, Patrick A. Naeger, Hayley K. Rogers, Safa Maharsi, Maryann Mbaka, A. Clinton White

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Fasciola hepatica is the most widely distributed trematode infection in the world. Control efforts may be hindered by the lack of diagnostic capacity especially in remote endemic areas. Polymerase chain reaction (PCR)-based methods offer high sensitivity and specificity but require expensive technology. However, the recombinase polymerase amplification (RPA) is an efficient isothermal method that eliminates the need for a thermal cycler and has a high deployment potential to resource-limited settings. We report on the characterization of RPA and PCR tests to detect Fasciola infection in clinical stool samples with low egg burdens. The sensitivity of the RPA and PCR were 87% and 66%, respectively. Both tests were 100% specific showing no cross-reactivity with trematode, cestode, or nematode parasites. In addition, RPA and PCR were able to detect 47% and 26% of infections not detected by microscopy, respectively. The RPA adapted to a lateral flow platform was more sensitive than gel-based detection of the reaction products. In conclusion, the Fasciola RPA is a highly sensitive and specific test to diagnose chronic infection using stool samples. The Fasciola RPA lateral flow has the potential for deployment to endemic areas after further characterization.

Original languageEnglish (US)
Pages (from-to)341-346
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume96
Issue number2
DOIs
StatePublished - 2017

Fingerprint

Fasciola hepatica
Recombinases
Real-Time Polymerase Chain Reaction
Fasciola
Polymerase Chain Reaction
Trematode Infections
Infection
Cestoda
Ovum
Microscopy
Parasites
Hot Temperature
Gels
Technology
Sensitivity and Specificity

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Recombinase polymerase amplification compared to real-time polymerase chain reaction test for the detection of fasciola hepatica in human stool. / Cabada, Miguel; Malaga, Jose L.; Castellanos, Alejandro; Bagwell, Kelli A.; Naeger, Patrick A.; Rogers, Hayley K.; Maharsi, Safa; Mbaka, Maryann; White, A. Clinton.

In: American Journal of Tropical Medicine and Hygiene, Vol. 96, No. 2, 2017, p. 341-346.

Research output: Contribution to journalArticle

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