Recombination and mutagenesis in rad6 mutants of Saccharomyces cerevisiae

Evidence for multiple functions of the RAD6 gene

Beth A. Montelone, Satya Prakash, Louise Prakash

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

The rad6-1 and rad6-3 mutants are highly UV sensitive and show an increase in spontaneous and UV induced mitotic heteroallelic recombination in diploids. Both rad6 mutants are proficient in spontaneous and UV induced unequal sister chromatid recombination in the reiterated ribosomal DNA sequence and are deficient in UV induced mutagenesis. In contrast to the above effects where both mutants appear similar, rad6-1 mutants are deficient in sporulation and meiotic recombination whereas rad6-3 mutants are proficient. The differential effects of these mutations indicate that the RAD6 gene is multifunctional. The possible role of the RAD6 gene in error prone excision repair of UV damage during the G1 phase of the cell cycle in addition to its role in postreplication repair is discussed.

Original languageEnglish
Pages (from-to)410-415
Number of pages6
JournalMGG Molecular & General Genetics
Volume184
Issue number3
DOIs
StatePublished - Dec 1981
Externally publishedYes

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Mutagenesis
Genetic Recombination
Saccharomyces cerevisiae
Genes
Chromatids
G1 Phase
Ribosomal DNA
Diploidy
DNA Repair
Cell Cycle
Mutation

ASJC Scopus subject areas

  • Genetics

Cite this

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AB - The rad6-1 and rad6-3 mutants are highly UV sensitive and show an increase in spontaneous and UV induced mitotic heteroallelic recombination in diploids. Both rad6 mutants are proficient in spontaneous and UV induced unequal sister chromatid recombination in the reiterated ribosomal DNA sequence and are deficient in UV induced mutagenesis. In contrast to the above effects where both mutants appear similar, rad6-1 mutants are deficient in sporulation and meiotic recombination whereas rad6-3 mutants are proficient. The differential effects of these mutations indicate that the RAD6 gene is multifunctional. The possible role of the RAD6 gene in error prone excision repair of UV damage during the G1 phase of the cell cycle in addition to its role in postreplication repair is discussed.

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