Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses

Scott Weaver, Wenli Kang, Yukio Shirako, Tillman Rümenapf, Ellen G. Strauss, James H. Strauss

Research output: Contribution to journalArticle

123 Citations (Scopus)

Abstract

Western equine encephalomyelitis (WEE) virus (Togaviridae: Alphavirus) was shown previously to have arisen by recombination between eastern equine encephalomyelitis (EEE)- and Sindbis-like viruses (C. S. Hahn, S. Lustig, E.G. Strauss, and J. H. Strauss, Proc. Natl. Acad. Sci. USA 85:5097-6001, 1988). We have now examined the recombinational history and evolution of all viruses belonging to the WEE antigenic complex, including the Buggy Creek, Fort Morgan, Highlands J, Sindbis, Babanki, Ockelbo, Kyzylagach, Whataroa, and Aura viruses, using nucleotide sequences derived from representative strains. Two regions of the genome were examined: sequences of 477 nucleotides from the C terminus of the E1 envelope glycoprotein gene which in WEE virus was derived from the Sindbis-like virus parent, and 517 nucleotide sequences at the C terminus of the nsP4 gene which in WEE virus was derived from the EEE-like virus parent. Trees based on the E1 region indicated that all members of the WEE virus complex comprise a monophyletic group. Most closely related to WEE viruses are other New World members of the complex: the Highlands J, Buggy Creek, and Fort Morgan viruses. More distantly related WEE complex viruses included the Old World Sindbis, Babanki, Ockelbo, Kyzylagach, and Whataroa viruses, as well as the New World Aura virus. Detailed analyses of 38 strains of WEE virus revealed at least 4 major lineages; two were represented by isolates from Argentina, one was from Brazil, and a fourth contained isolates from many locations in South and North America as well as Cuba. Trees based on the nsP4 gene indicated that all New World WEE complex viruses except Aura virus are recombinants derived from EEE- and Sindbis-like virus ancestors. In contrast, the Old World members of the WEE complex, as well as Aura virus, did not appear to have recombinant genomes. Using an evolutionary rate estimate (2.8 x 10-4 substitutions per nucleotide per year) obtained from E1-3' sequences of WEE viruses, we estimated that the recombination event occurred in the New World 1,300 to 1,900 years ago. This suggests that the alphaviruses originated in the New World a few thousand years ago.

Original languageEnglish (US)
Pages (from-to)613-623
Number of pages11
JournalJournal of Virology
Volume71
Issue number1
StatePublished - 1997

Fingerprint

Western Equine Encephalomyelitis
Western equine encephalomyelitis
Western Equine Encephalitis Viruses
Sindbis virus
Alphavirus
Western equine encephalitis virus
Molecular Evolution
Aura virus
History
history
Sindbis Virus
Eastern equine encephalomyelitis
viruses
Whataroa virus
Viruses
Epilepsy
Eastern Equine Encephalomyelitis
nucleotide sequences
Highlands J virus
Fort Morgan virus

ASJC Scopus subject areas

  • Immunology

Cite this

Weaver, S., Kang, W., Shirako, Y., Rümenapf, T., Strauss, E. G., & Strauss, J. H. (1997). Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses. Journal of Virology, 71(1), 613-623.

Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses. / Weaver, Scott; Kang, Wenli; Shirako, Yukio; Rümenapf, Tillman; Strauss, Ellen G.; Strauss, James H.

In: Journal of Virology, Vol. 71, No. 1, 1997, p. 613-623.

Research output: Contribution to journalArticle

Weaver, S, Kang, W, Shirako, Y, Rümenapf, T, Strauss, EG & Strauss, JH 1997, 'Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses', Journal of Virology, vol. 71, no. 1, pp. 613-623.
Weaver, Scott ; Kang, Wenli ; Shirako, Yukio ; Rümenapf, Tillman ; Strauss, Ellen G. ; Strauss, James H. / Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses. In: Journal of Virology. 1997 ; Vol. 71, No. 1. pp. 613-623.
@article{adad214b30624863a349bb78ddc4f625,
title = "Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses",
abstract = "Western equine encephalomyelitis (WEE) virus (Togaviridae: Alphavirus) was shown previously to have arisen by recombination between eastern equine encephalomyelitis (EEE)- and Sindbis-like viruses (C. S. Hahn, S. Lustig, E.G. Strauss, and J. H. Strauss, Proc. Natl. Acad. Sci. USA 85:5097-6001, 1988). We have now examined the recombinational history and evolution of all viruses belonging to the WEE antigenic complex, including the Buggy Creek, Fort Morgan, Highlands J, Sindbis, Babanki, Ockelbo, Kyzylagach, Whataroa, and Aura viruses, using nucleotide sequences derived from representative strains. Two regions of the genome were examined: sequences of 477 nucleotides from the C terminus of the E1 envelope glycoprotein gene which in WEE virus was derived from the Sindbis-like virus parent, and 517 nucleotide sequences at the C terminus of the nsP4 gene which in WEE virus was derived from the EEE-like virus parent. Trees based on the E1 region indicated that all members of the WEE virus complex comprise a monophyletic group. Most closely related to WEE viruses are other New World members of the complex: the Highlands J, Buggy Creek, and Fort Morgan viruses. More distantly related WEE complex viruses included the Old World Sindbis, Babanki, Ockelbo, Kyzylagach, and Whataroa viruses, as well as the New World Aura virus. Detailed analyses of 38 strains of WEE virus revealed at least 4 major lineages; two were represented by isolates from Argentina, one was from Brazil, and a fourth contained isolates from many locations in South and North America as well as Cuba. Trees based on the nsP4 gene indicated that all New World WEE complex viruses except Aura virus are recombinants derived from EEE- and Sindbis-like virus ancestors. In contrast, the Old World members of the WEE complex, as well as Aura virus, did not appear to have recombinant genomes. Using an evolutionary rate estimate (2.8 x 10-4 substitutions per nucleotide per year) obtained from E1-3' sequences of WEE viruses, we estimated that the recombination event occurred in the New World 1,300 to 1,900 years ago. This suggests that the alphaviruses originated in the New World a few thousand years ago.",
author = "Scott Weaver and Wenli Kang and Yukio Shirako and Tillman R{\"u}menapf and Strauss, {Ellen G.} and Strauss, {James H.}",
year = "1997",
language = "English (US)",
volume = "71",
pages = "613--623",
journal = "Journal of Virology",
issn = "0022-538X",
publisher = "American Society for Microbiology",
number = "1",

}

TY - JOUR

T1 - Recombinational history and molecular evolution of western equine encephalomyelitis complex alphaviruses

AU - Weaver, Scott

AU - Kang, Wenli

AU - Shirako, Yukio

AU - Rümenapf, Tillman

AU - Strauss, Ellen G.

AU - Strauss, James H.

PY - 1997

Y1 - 1997

N2 - Western equine encephalomyelitis (WEE) virus (Togaviridae: Alphavirus) was shown previously to have arisen by recombination between eastern equine encephalomyelitis (EEE)- and Sindbis-like viruses (C. S. Hahn, S. Lustig, E.G. Strauss, and J. H. Strauss, Proc. Natl. Acad. Sci. USA 85:5097-6001, 1988). We have now examined the recombinational history and evolution of all viruses belonging to the WEE antigenic complex, including the Buggy Creek, Fort Morgan, Highlands J, Sindbis, Babanki, Ockelbo, Kyzylagach, Whataroa, and Aura viruses, using nucleotide sequences derived from representative strains. Two regions of the genome were examined: sequences of 477 nucleotides from the C terminus of the E1 envelope glycoprotein gene which in WEE virus was derived from the Sindbis-like virus parent, and 517 nucleotide sequences at the C terminus of the nsP4 gene which in WEE virus was derived from the EEE-like virus parent. Trees based on the E1 region indicated that all members of the WEE virus complex comprise a monophyletic group. Most closely related to WEE viruses are other New World members of the complex: the Highlands J, Buggy Creek, and Fort Morgan viruses. More distantly related WEE complex viruses included the Old World Sindbis, Babanki, Ockelbo, Kyzylagach, and Whataroa viruses, as well as the New World Aura virus. Detailed analyses of 38 strains of WEE virus revealed at least 4 major lineages; two were represented by isolates from Argentina, one was from Brazil, and a fourth contained isolates from many locations in South and North America as well as Cuba. Trees based on the nsP4 gene indicated that all New World WEE complex viruses except Aura virus are recombinants derived from EEE- and Sindbis-like virus ancestors. In contrast, the Old World members of the WEE complex, as well as Aura virus, did not appear to have recombinant genomes. Using an evolutionary rate estimate (2.8 x 10-4 substitutions per nucleotide per year) obtained from E1-3' sequences of WEE viruses, we estimated that the recombination event occurred in the New World 1,300 to 1,900 years ago. This suggests that the alphaviruses originated in the New World a few thousand years ago.

AB - Western equine encephalomyelitis (WEE) virus (Togaviridae: Alphavirus) was shown previously to have arisen by recombination between eastern equine encephalomyelitis (EEE)- and Sindbis-like viruses (C. S. Hahn, S. Lustig, E.G. Strauss, and J. H. Strauss, Proc. Natl. Acad. Sci. USA 85:5097-6001, 1988). We have now examined the recombinational history and evolution of all viruses belonging to the WEE antigenic complex, including the Buggy Creek, Fort Morgan, Highlands J, Sindbis, Babanki, Ockelbo, Kyzylagach, Whataroa, and Aura viruses, using nucleotide sequences derived from representative strains. Two regions of the genome were examined: sequences of 477 nucleotides from the C terminus of the E1 envelope glycoprotein gene which in WEE virus was derived from the Sindbis-like virus parent, and 517 nucleotide sequences at the C terminus of the nsP4 gene which in WEE virus was derived from the EEE-like virus parent. Trees based on the E1 region indicated that all members of the WEE virus complex comprise a monophyletic group. Most closely related to WEE viruses are other New World members of the complex: the Highlands J, Buggy Creek, and Fort Morgan viruses. More distantly related WEE complex viruses included the Old World Sindbis, Babanki, Ockelbo, Kyzylagach, and Whataroa viruses, as well as the New World Aura virus. Detailed analyses of 38 strains of WEE virus revealed at least 4 major lineages; two were represented by isolates from Argentina, one was from Brazil, and a fourth contained isolates from many locations in South and North America as well as Cuba. Trees based on the nsP4 gene indicated that all New World WEE complex viruses except Aura virus are recombinants derived from EEE- and Sindbis-like virus ancestors. In contrast, the Old World members of the WEE complex, as well as Aura virus, did not appear to have recombinant genomes. Using an evolutionary rate estimate (2.8 x 10-4 substitutions per nucleotide per year) obtained from E1-3' sequences of WEE viruses, we estimated that the recombination event occurred in the New World 1,300 to 1,900 years ago. This suggests that the alphaviruses originated in the New World a few thousand years ago.

UR - http://www.scopus.com/inward/record.url?scp=0031060163&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031060163&partnerID=8YFLogxK

M3 - Article

VL - 71

SP - 613

EP - 623

JO - Journal of Virology

JF - Journal of Virology

SN - 0022-538X

IS - 1

ER -