Recovery of live virus after storage at ambient temperature using ViveST™

Kelli L. Barr, Ali M. Messenger, Benjamin D. Anderson, John A. Friary, Gary L. Heil, Kristy Reece, Gregory C. Gray

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


Background: A major impediment to performing virological field studies in developing nations is the lack of ultra-low freezers as well as the expense and difficulty of shipping frozen samples. A commercially available product, ViveST™, was developed to preserve nucleic acids at ambient temperature for use in specimen storage and transportation. However, its applications as a viral storage, transport and recovery device have not been evaluated. Objective: To examine the ability of ViveST to preserve live virus following storage at ambient temperature. Study design: A panel of six viruses was stored at ambient temperature (∼22. °C) in ViveST with fetal bovine serum (FBS), or ViveST with minimal essential media (MEM) and compared with virus stored in universal transport media (M4RT), MEM, and FBS alone. Stored viruses included: human adenovirus (14p), dengue virus 2 (16608), echovirus 3 (Morrisey), human rhinovirus 15 (1734), Coxsackie virus B5 (Faulkner), and herpes simplex virus 1 (HF). After 7 days storage at ambient temperature, virus recovery was measured via titration using viral plaque assays or focus-forming unit assays. Results: Viral titer studies indicate that ViveST with either FBS or M4RT preserved/recovered 5 different viruses for 1 week at ambient temperature. MEM preserved 4 viruses while FBS and ViveST with MEM preserved 3 viruses each. Statistical analyses indicate that M4RT and ViveST with FBS preserved significantly more virus than the other treatments. Conclusions: These data suggest that ViveST with either FBS or M4RT may be useful in field specimen collection scenarios where ultra-cold storage is not available.

Original languageEnglish (US)
Pages (from-to)57-61
Number of pages5
JournalJournal of Clinical Virology
Issue number1
StatePublished - Jan 2013
Externally publishedYes


  • Preservation
  • Recovery
  • Storage
  • Transport
  • Virus

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases


Dive into the research topics of 'Recovery of live virus after storage at ambient temperature using ViveST™'. Together they form a unique fingerprint.

Cite this