Redox properties of the A-domain of the HMGB1 protein

Debashish Sahu, Priyanka Debnath, Yuki Takayama, Junji Iwahara

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The High Mobility Group B1 (HMGB1) protein plays important roles in both intracellular (reductive) and extracellular (oxidative) environments. We have carried out quantitative investigations of the redox chemistry involving Cys22 and Cys44 of the HMGB1 A-domain, which form an intramolecular disulfide bond. Using NMR spectroscopy, we analyzed the real-time kinetics of the redox reactions for the A-domain in glutathione and thioredoxin systems, and also determined the standard redox potential. Thermodynamic experiments showed that the Cys22-Cys44 disulfide bond stabilizes the folded state of the protein. These data suggest that the oxidized HMGB1 may accumulate even in cells under oxidative stress. Structured summary: MINT-6795963:txn (uniprotkb:P10599) and HMGB1 (uniprotkb:P09429) bind (MI:0408) by nuclear magnetic resonance (MI:0077).

Original languageEnglish (US)
Pages (from-to)3973-3978
Number of pages6
JournalFEBS Letters
Volume582
Issue number29
DOIs
StatePublished - Dec 10 2008

Fingerprint

High Mobility Group Proteins
Disulfides
Oxidation-Reduction
Thioredoxins
Oxidative stress
Redox reactions
Magnetic Resonance Spectroscopy
Nuclear magnetic resonance spectroscopy
Glutathione
Proteins
Nuclear magnetic resonance
Thermodynamics
Kinetics
Oxidative Stress
Experiments

Keywords

  • Disulfide bond
  • HMGB1
  • Kinetics
  • NMR spectroscopy
  • Redox
  • Thermodynamics

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Cell Biology
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Redox properties of the A-domain of the HMGB1 protein. / Sahu, Debashish; Debnath, Priyanka; Takayama, Yuki; Iwahara, Junji.

In: FEBS Letters, Vol. 582, No. 29, 10.12.2008, p. 3973-3978.

Research output: Contribution to journalArticle

Sahu, Debashish ; Debnath, Priyanka ; Takayama, Yuki ; Iwahara, Junji. / Redox properties of the A-domain of the HMGB1 protein. In: FEBS Letters. 2008 ; Vol. 582, No. 29. pp. 3973-3978.
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