Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas

Aaron C. Brault, Stanley A. Langevin, Wanichaya N. Ramey, Ying Fang, David Beasley, Christopher M. Barker, Todd A. Sanders, William K. Reisen, Alan Barrett, Richard A. Bowen

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

Original languageEnglish (US)
Pages (from-to)758-767
Number of pages10
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume85
Issue number4
DOIs
StatePublished - Oct 2011

Fingerprint

West Nile virus
Viremia
Mexico
Crows
Virulence
Glycosylation
Sparrows
Latin America
Genotype
Phenotype

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas. / Brault, Aaron C.; Langevin, Stanley A.; Ramey, Wanichaya N.; Fang, Ying; Beasley, David; Barker, Christopher M.; Sanders, Todd A.; Reisen, William K.; Barrett, Alan; Bowen, Richard A.

In: American Journal of Tropical Medicine and Hygiene, Vol. 85, No. 4, 10.2011, p. 758-767.

Research output: Contribution to journalArticle

Brault, AC, Langevin, SA, Ramey, WN, Fang, Y, Beasley, D, Barker, CM, Sanders, TA, Reisen, WK, Barrett, A & Bowen, RA 2011, 'Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas', American Journal of Tropical Medicine and Hygiene, vol. 85, no. 4, pp. 758-767. https://doi.org/10.4269/ajtmh.2011.10-0439
Brault, Aaron C. ; Langevin, Stanley A. ; Ramey, Wanichaya N. ; Fang, Ying ; Beasley, David ; Barker, Christopher M. ; Sanders, Todd A. ; Reisen, William K. ; Barrett, Alan ; Bowen, Richard A. / Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas. In: American Journal of Tropical Medicine and Hygiene. 2011 ; Vol. 85, No. 4. pp. 758-767.
@article{daa4369ef5264f44866f29705b4cae4c,
title = "Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas",
abstract = "A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.",
author = "Brault, {Aaron C.} and Langevin, {Stanley A.} and Ramey, {Wanichaya N.} and Ying Fang and David Beasley and Barker, {Christopher M.} and Sanders, {Todd A.} and Reisen, {William K.} and Alan Barrett and Bowen, {Richard A.}",
year = "2011",
month = "10",
doi = "10.4269/ajtmh.2011.10-0439",
language = "English (US)",
volume = "85",
pages = "758--767",
journal = "American Journal of Tropical Medicine and Hygiene",
issn = "0002-9637",
publisher = "American Society of Tropical Medicine and Hygiene",
number = "4",

}

TY - JOUR

T1 - Reduced avian virulence and viremia of West Nile virus isolates from Mexico and Texas

AU - Brault, Aaron C.

AU - Langevin, Stanley A.

AU - Ramey, Wanichaya N.

AU - Fang, Ying

AU - Beasley, David

AU - Barker, Christopher M.

AU - Sanders, Todd A.

AU - Reisen, William K.

AU - Barrett, Alan

AU - Bowen, Richard A.

PY - 2011/10

Y1 - 2011/10

N2 - A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

AB - A West Nile virus (WNV) isolate from Mexico (TM171-03) and BIRD1153, a unique genotype from Texas, have exhibited reduced murine neuroinvasive phenotypes. To determine if murine neuroinvasive capacity equates to avian virulence potential, American crow ( Corvus brachyrhynchos ) and house sparrows ( Passer domesticus ) were experimentally inoculated with representative murine neuroinvasive/non-neuroinvasive strains. In both avian species, a plaque variant from Mexico that was E-glycosylation competent produced higher viremias than an E-glycosylation-incompetent variant, indicating the potential importance of E-glycosylation for avian replication. The murine non-neuroinvasive BIRD1153 strain was significantly attenuated in American crows but not house sparrows when compared with the murine neuroinvasive Texas strain. Despite the loss of murine neuroinvasive properties of nonglycosylated variants from Mexico, our data indicate avian replication potential of these strains and that unique WNV virulence characteristics exist between murine and avian models. The implications of reduced avian replication of variants from Mexico for restricted WNV transmission in Latin America is discussed.

UR - http://www.scopus.com/inward/record.url?scp=80053907544&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053907544&partnerID=8YFLogxK

U2 - 10.4269/ajtmh.2011.10-0439

DO - 10.4269/ajtmh.2011.10-0439

M3 - Article

VL - 85

SP - 758

EP - 767

JO - American Journal of Tropical Medicine and Hygiene

JF - American Journal of Tropical Medicine and Hygiene

SN - 0002-9637

IS - 4

ER -