Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage

Elena Titova; Robert P. Ostrowski; Christopher G. Kevil; Wenni Tong; Hugo Rojas; Lawrence C. Sowers; John H. Zhang; Jiping Tang

doi:10.1002/jnr.21762

Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage

Elena Titova, Robert P. Ostrowski, Christopher G. Kevil, Wenni Tong, Hugo Rojas, Lawrence C. Sowers, John H. Zhang, Jiping Tang

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18^+/+ mice and CD18^-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

Original language	English (US)
Pages (from-to)	3240-3245
Number of pages	6
Journal	Journal of Neuroscience Research
Volume	86
Issue number	14
DOIs	https://doi.org/10.1002/jnr.21762
State	Published - 2008
Externally published	Yes

Keywords

Brain edema
CD18 mice
Collagenase
Inflammation
Intracerebral hemorrhage

ASJC Scopus subject areas

Cellular and Molecular Neuroscience

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10.1002/jnr.21762

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title = "Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage",

abstract = "Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitr{\o}tyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.",

keywords = "Brain edema, CD18 mice, Collagenase, Inflammation, Intracerebral hemorrhage",

author = "Elena Titova and Ostrowski, {Robert P.} and Kevil, {Christopher G.} and Wenni Tong and Hugo Rojas and Sowers, {Lawrence C.} and Zhang, {John H.} and Jiping Tang",

year = "2008",

doi = "10.1002/jnr.21762",

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AU - Titova, Elena

AU - Ostrowski, Robert P.

AU - Kevil, Christopher G.

AU - Tong, Wenni

AU - Rojas, Hugo

AU - Sowers, Lawrence C.

AU - Zhang, John H.

AU - Tang, Jiping

PY - 2008

Y1 - 2008

N2 - Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

AB - Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

KW - Brain edema

KW - CD18 mice

KW - Collagenase

KW - Inflammation

KW - Intracerebral hemorrhage

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JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

IS - 14

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Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage

Abstract

Keywords

ASJC Scopus subject areas

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