Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage

Elena Titova, Robert P. Ostrowski, Christopher G. Kevil, Wenni Tong, Hugo Rojas, Lawrence Sowers, John H. Zhang, Jiping Tang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

Original languageEnglish
Pages (from-to)3240-3245
Number of pages6
JournalJournal of Neuroscience Research
Volume86
Issue number14
DOIs
StatePublished - 2008
Externally publishedYes

Fingerprint

Cerebral Hemorrhage
Brain Injuries
Peroxidase
Brain Edema
Knockout Mice
Leukocytes
Mortality
Collagenases
Reperfusion Injury
Neutrophils
Injections
Brain

Keywords

  • Brain edema
  • CD18 mice
  • Collagenase
  • Inflammation
  • Intracerebral hemorrhage

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience

Cite this

Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage. / Titova, Elena; Ostrowski, Robert P.; Kevil, Christopher G.; Tong, Wenni; Rojas, Hugo; Sowers, Lawrence; Zhang, John H.; Tang, Jiping.

In: Journal of Neuroscience Research, Vol. 86, No. 14, 2008, p. 3240-3245.

Research output: Contribution to journalArticle

Titova, E, Ostrowski, RP, Kevil, CG, Tong, W, Rojas, H, Sowers, L, Zhang, JH & Tang, J 2008, 'Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage', Journal of Neuroscience Research, vol. 86, no. 14, pp. 3240-3245. https://doi.org/10.1002/jnr.21762
Titova, Elena ; Ostrowski, Robert P. ; Kevil, Christopher G. ; Tong, Wenni ; Rojas, Hugo ; Sowers, Lawrence ; Zhang, John H. ; Tang, Jiping. / Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage. In: Journal of Neuroscience Research. 2008 ; Vol. 86, No. 14. pp. 3240-3245.
@article{e5424ecc4d974634b7f77f38c81744b7,
title = "Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage",
abstract = "Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitr{\o}tyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.",
keywords = "Brain edema, CD18 mice, Collagenase, Inflammation, Intracerebral hemorrhage",
author = "Elena Titova and Ostrowski, {Robert P.} and Kevil, {Christopher G.} and Wenni Tong and Hugo Rojas and Lawrence Sowers and Zhang, {John H.} and Jiping Tang",
year = "2008",
doi = "10.1002/jnr.21762",
language = "English",
volume = "86",
pages = "3240--3245",
journal = "Journal of Neuroscience Research",
issn = "0360-4012",
publisher = "Wiley-Liss Inc.",
number = "14",

}

TY - JOUR

T1 - Reduced brain injury in CD18-deficient mice after experimental intracerebral hemorrhage

AU - Titova, Elena

AU - Ostrowski, Robert P.

AU - Kevil, Christopher G.

AU - Tong, Wenni

AU - Rojas, Hugo

AU - Sowers, Lawrence

AU - Zhang, John H.

AU - Tang, Jiping

PY - 2008

Y1 - 2008

N2 - Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

AB - Many studies have indicated leukocytes are a major contributor to brain injuries caused by intracerebral hemorrhage (ICH). Leukocyte-expressed CD18 is important for neutrophil-endothelial interactions in the vasculature, and CD18 deficiency protects against ischemia-reperfusion injury. We investigated whether CD18 deficiency provides protection against ICH-induced brain injury. Male wild-type (WT) CD18+/+ mice and CD18-/- -knockout mice were used in this study. ICH was induced by a collagenase injection. Mortality, neurological function, brain edema, and myeloperoxidase (MPO) activity as well as tissue expression of nitrotyrosine and MPO were evaluated 24 hr after ICH. We discovered significantly reduced brain edema and diminished mortality with a concomitant decrease in MPO and nitrøtyrosine immunoreactivity in brains of CD18-knockout mice. _cd 2008 Wiley-Liss, Inc.

KW - Brain edema

KW - CD18 mice

KW - Collagenase

KW - Inflammation

KW - Intracerebral hemorrhage

UR - http://www.scopus.com/inward/record.url?scp=57049188820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=57049188820&partnerID=8YFLogxK

U2 - 10.1002/jnr.21762

DO - 10.1002/jnr.21762

M3 - Article

C2 - 18615643

AN - SCOPUS:57049188820

VL - 86

SP - 3240

EP - 3245

JO - Journal of Neuroscience Research

JF - Journal of Neuroscience Research

SN - 0360-4012

IS - 14

ER -