Reduced CD1d expression in colonic epithelium in microscopic colitis

Yimin Ge, Bill A. Rampy, Hanlin L. Wang, Shu Yuan Xiao

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

CD1d is a member of a major histocompatibility complex class I-like molecule family. Its function may involve presenting microbial nonpeptide or lipidic antigens to T lymphocytes, therefore to serve as an important factor in normal mucosal immunity of the gastrointestinal tract. In this study, the expression level of CD1d in microscopic colitis (ie, collagenous and lymphocytic colitis) was examined, and compared with that in normal colonic mucosa. Formalin-fixed, paraffin-embedded colon biopsies with diagnosis of lymphocytic colitis (19 cases), collagenous colitis (6 cases), and no pathologic change (20 cases) were studied immunohistochemically using monoclonal antibodies against human CD1d, CD3, CD4, and CD8. CD1d staining in the epithelium and lamina propria was graded along a scale of 0 to 4. Intraepithelial CD3-positive lymphocytes were counted in an area of 300 epithelial cells for each specimen. The results show that CD1d was expressed in normal colonic epithelial cells, primarily on the basolateral membranes with a concentrated intracellular pool in the subnuclear region. The expression level was markedly reduced in both lymphocytic colitis (P<0.001) and collagenous colitis (P<0.001), along with a significant increase in the number of intraepithelial CD3/CD8 lymphocytes (P<0.001). These findings suggest that microscopic colitis is associated with decreased epithelial expression of CD1d, an important immunoregulatory molecule in the gastrointestinal tract.

Original languageEnglish (US)
Pages (from-to)309-313
Number of pages5
JournalApplied Immunohistochemistry and Molecular Morphology
Volume14
Issue number3
DOIs
StatePublished - Sep 2006

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Lymphocytic Colitis
Microscopic Colitis
Collagenous Colitis
Epithelium
Gastrointestinal Tract
Mucous Membrane
Epithelial Cells
Lymphocytes
Mucosal Immunity
Major Histocompatibility Complex
Paraffin
Formaldehyde
Colon
Monoclonal Antibodies
Staining and Labeling
T-Lymphocytes
Biopsy
Antigens
Membranes

Keywords

  • CD1d
  • Chronic colitis
  • Collagenous colitis
  • Inflammatory bowel disease
  • Lymphocytic colitis
  • Microscopic colitis
  • Mucosal immunity

ASJC Scopus subject areas

  • Anatomy
  • Medical Laboratory Technology

Cite this

Reduced CD1d expression in colonic epithelium in microscopic colitis. / Ge, Yimin; Rampy, Bill A.; Wang, Hanlin L.; Xiao, Shu Yuan.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 14, No. 3, 09.2006, p. 309-313.

Research output: Contribution to journalArticle

Ge, Yimin ; Rampy, Bill A. ; Wang, Hanlin L. ; Xiao, Shu Yuan. / Reduced CD1d expression in colonic epithelium in microscopic colitis. In: Applied Immunohistochemistry and Molecular Morphology. 2006 ; Vol. 14, No. 3. pp. 309-313.
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