Abstract
The effects of luminal bile salts on plasma levels of cholecystokinin (CCK) and growth of the pancreas in mice were studied. Nonfasting levels of plasma CCK in control mice were 8.1 ± 1.5 pM. Feeding mice a 0.5% (wt/wt) sodium taurocholate-supplemented diet for 1 wk significantly lowered nonfasting levels of plasma CCK to 4.1 ± 0.5 pM and decreased the total contents of pancreatic DNA by 22%, RNA by 25%, and protein by 24%. All of the inhibitory effects of taurocholate on pancreatic growth were comletely reversed by the simultaneous administration of CCK-8 (3 μg/kg, 3 times daily). In contrast, intraluminal neutralization of endogenous bile salts by feeding a 4% (wt/wt) cholestyramine-supplemented diet for 1 wk significantly elevated nonfasting levels of plasma CCK to 14.7 ± 1.5 pM and increased the total contents of pancreatic DNA by 34%, RNA by 40%, and protein by 35%. All of the stimulatory actions of cholestyramine on pancreatic growth were completely abolished by the administration of the highly potent and specific CCK-receptor antagonist L364,718 (1 mg/kg, twice daily). These findings, therefore, indicate that bile salts appear to play a physiological role in pancreatic growth by regulation of plasma levels of CCK.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Gastrointestinal and Liver Physiology |
| Volume | 259 |
| Issue number | 1 22-1 |
| State | Published - 1990 |
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Keywords
- cholestyramine
- L365,718
- sodium taurocholate
ASJC Scopus subject areas
- Physiology
- Gastroenterology
Cite this
Reduced cholecystokinin mediates the inhibition of pancreatic growth induced by bile salts. / Gomez, Guillermo; Townsend, Courtney; Green, D. W.; Rajaraman, S.; Greeley, G. H.; Thompson, J. C.
In: American Journal of Physiology - Gastrointestinal and Liver Physiology, Vol. 259, No. 1 22-1, 1990.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduced cholecystokinin mediates the inhibition of pancreatic growth induced by bile salts
AU - Gomez, Guillermo
AU - Townsend, Courtney
AU - Green, D. W.
AU - Rajaraman, S.
AU - Greeley, G. H.
AU - Thompson, J. C.
PY - 1990
Y1 - 1990
N2 - The effects of luminal bile salts on plasma levels of cholecystokinin (CCK) and growth of the pancreas in mice were studied. Nonfasting levels of plasma CCK in control mice were 8.1 ± 1.5 pM. Feeding mice a 0.5% (wt/wt) sodium taurocholate-supplemented diet for 1 wk significantly lowered nonfasting levels of plasma CCK to 4.1 ± 0.5 pM and decreased the total contents of pancreatic DNA by 22%, RNA by 25%, and protein by 24%. All of the inhibitory effects of taurocholate on pancreatic growth were comletely reversed by the simultaneous administration of CCK-8 (3 μg/kg, 3 times daily). In contrast, intraluminal neutralization of endogenous bile salts by feeding a 4% (wt/wt) cholestyramine-supplemented diet for 1 wk significantly elevated nonfasting levels of plasma CCK to 14.7 ± 1.5 pM and increased the total contents of pancreatic DNA by 34%, RNA by 40%, and protein by 35%. All of the stimulatory actions of cholestyramine on pancreatic growth were completely abolished by the administration of the highly potent and specific CCK-receptor antagonist L364,718 (1 mg/kg, twice daily). These findings, therefore, indicate that bile salts appear to play a physiological role in pancreatic growth by regulation of plasma levels of CCK.
AB - The effects of luminal bile salts on plasma levels of cholecystokinin (CCK) and growth of the pancreas in mice were studied. Nonfasting levels of plasma CCK in control mice were 8.1 ± 1.5 pM. Feeding mice a 0.5% (wt/wt) sodium taurocholate-supplemented diet for 1 wk significantly lowered nonfasting levels of plasma CCK to 4.1 ± 0.5 pM and decreased the total contents of pancreatic DNA by 22%, RNA by 25%, and protein by 24%. All of the inhibitory effects of taurocholate on pancreatic growth were comletely reversed by the simultaneous administration of CCK-8 (3 μg/kg, 3 times daily). In contrast, intraluminal neutralization of endogenous bile salts by feeding a 4% (wt/wt) cholestyramine-supplemented diet for 1 wk significantly elevated nonfasting levels of plasma CCK to 14.7 ± 1.5 pM and increased the total contents of pancreatic DNA by 34%, RNA by 40%, and protein by 35%. All of the stimulatory actions of cholestyramine on pancreatic growth were completely abolished by the administration of the highly potent and specific CCK-receptor antagonist L364,718 (1 mg/kg, twice daily). These findings, therefore, indicate that bile salts appear to play a physiological role in pancreatic growth by regulation of plasma levels of CCK.
KW - cholestyramine
KW - L365,718
KW - sodium taurocholate
UR - http://www.scopus.com/inward/record.url?scp=0025363358&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025363358&partnerID=8YFLogxK
M3 - Article
C2 - 1695489
AN - SCOPUS:0025363358
VL - 259
JO - American Journal of Physiology - Endocrinology and Metabolism
JF - American Journal of Physiology - Endocrinology and Metabolism
SN - 0193-1849
IS - 1 22-1
ER -