Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice

Kasra A. Rezaei, Hassanain S. Toma, Jiyang Cai, John S. Penn, Paul Sternberg, Stephen J. Kim

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Purpose. To assess the degree of laser-induced choroidal neo-vascular membrane formation in wild-type (WT) and COX-2 null mice and to measure vascular endothelial growth factor (VEGF), interleukin (IL)-1, and tumor necrosis factor (TNF)-α levels in the retina and choroid. Methods. Four laser burns were placed in each eye of WT and COX-2 null mice to induce choroidal neovascularization. Fluorescein angiography (FA) was performed at 14 days, and retinal pigment epithelium-choroid-sclera (choroidal) flat mounts were prepared. The retina and choroid were isolated from WT and COX-2 null mice at 24, 72, and 168 hours after laser photocoagulation and from unlasered eyes and were tested for VEGF, IL-1β, and TNF-α. Results. COX-2 null mice demonstrated 58% (P = 0.001) and 48% (P = 0.001) reductions in CNV formation on FA and choroidal flat mounts, respectively, compared with WT mice. For unlasered mice, mean VEGF concentrations in the retina and choroid were 1.2 ± 0.42 pg/mg protein for WT but only 0.42 ±0.2 pg/mg protein for COX-2 null mice (P < 0.05). After laser photocoagulation, WT mice showed significantly greater VEGF and IL-β expression in the retina and choroid by 168 hours (P < 0.05) and 72 hours (P < 0.05), respectively, compared with COX-2 null mice. Conclusions. COX-2 null mice exhibited significantly less choroidal neovascular membrane formation associated with reduced expression of VEGF. The results of this study suggest that COX-2 modulates VEGF expression in CNV and implicates a potential therapeutic role for nonsteroidal anti-inflammatory drugs.

Original languageEnglish (US)
Pages (from-to)701-707
Number of pages7
JournalInvestigative Ophthalmology and Visual Science
Volume52
Issue number2
DOIs
StatePublished - Feb 2011
Externally publishedYes

Fingerprint

Choroid
Vascular Endothelial Growth Factor A
Membranes
Retina
Lasers
Fluorescein Angiography
Light Coagulation
Interleukin-1
Tumor Necrosis Factor-alpha
Mouse Ptgs2 protein
Choroidal Neovascularization
Sclera
Retinal Pigment Epithelium
Interleukins
Burns
Blood Vessels
Anti-Inflammatory Agents
Pharmaceutical Preparations
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience
  • Medicine(all)

Cite this

Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice. / Rezaei, Kasra A.; Toma, Hassanain S.; Cai, Jiyang; Penn, John S.; Sternberg, Paul; Kim, Stephen J.

In: Investigative Ophthalmology and Visual Science, Vol. 52, No. 2, 02.2011, p. 701-707.

Research output: Contribution to journalArticle

Rezaei, Kasra A. ; Toma, Hassanain S. ; Cai, Jiyang ; Penn, John S. ; Sternberg, Paul ; Kim, Stephen J. / Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice. In: Investigative Ophthalmology and Visual Science. 2011 ; Vol. 52, No. 2. pp. 701-707.
@article{d55782a9ba7a4337ab18fd899a0f178f,
title = "Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice",
abstract = "Purpose. To assess the degree of laser-induced choroidal neo-vascular membrane formation in wild-type (WT) and COX-2 null mice and to measure vascular endothelial growth factor (VEGF), interleukin (IL)-1, and tumor necrosis factor (TNF)-α levels in the retina and choroid. Methods. Four laser burns were placed in each eye of WT and COX-2 null mice to induce choroidal neovascularization. Fluorescein angiography (FA) was performed at 14 days, and retinal pigment epithelium-choroid-sclera (choroidal) flat mounts were prepared. The retina and choroid were isolated from WT and COX-2 null mice at 24, 72, and 168 hours after laser photocoagulation and from unlasered eyes and were tested for VEGF, IL-1β, and TNF-α. Results. COX-2 null mice demonstrated 58{\%} (P = 0.001) and 48{\%} (P = 0.001) reductions in CNV formation on FA and choroidal flat mounts, respectively, compared with WT mice. For unlasered mice, mean VEGF concentrations in the retina and choroid were 1.2 ± 0.42 pg/mg protein for WT but only 0.42 ±0.2 pg/mg protein for COX-2 null mice (P < 0.05). After laser photocoagulation, WT mice showed significantly greater VEGF and IL-β expression in the retina and choroid by 168 hours (P < 0.05) and 72 hours (P < 0.05), respectively, compared with COX-2 null mice. Conclusions. COX-2 null mice exhibited significantly less choroidal neovascular membrane formation associated with reduced expression of VEGF. The results of this study suggest that COX-2 modulates VEGF expression in CNV and implicates a potential therapeutic role for nonsteroidal anti-inflammatory drugs.",
author = "Rezaei, {Kasra A.} and Toma, {Hassanain S.} and Jiyang Cai and Penn, {John S.} and Paul Sternberg and Kim, {Stephen J.}",
year = "2011",
month = "2",
doi = "10.1167/iovs.10-6319",
language = "English (US)",
volume = "52",
pages = "701--707",
journal = "Investigative Ophthalmology and Visual Science",
issn = "0146-0404",
publisher = "Association for Research in Vision and Ophthalmology Inc.",
number = "2",

}

TY - JOUR

T1 - Reduced choroidal neovascular membrane formation in cyclooxygenase-2 null mice

AU - Rezaei, Kasra A.

AU - Toma, Hassanain S.

AU - Cai, Jiyang

AU - Penn, John S.

AU - Sternberg, Paul

AU - Kim, Stephen J.

PY - 2011/2

Y1 - 2011/2

N2 - Purpose. To assess the degree of laser-induced choroidal neo-vascular membrane formation in wild-type (WT) and COX-2 null mice and to measure vascular endothelial growth factor (VEGF), interleukin (IL)-1, and tumor necrosis factor (TNF)-α levels in the retina and choroid. Methods. Four laser burns were placed in each eye of WT and COX-2 null mice to induce choroidal neovascularization. Fluorescein angiography (FA) was performed at 14 days, and retinal pigment epithelium-choroid-sclera (choroidal) flat mounts were prepared. The retina and choroid were isolated from WT and COX-2 null mice at 24, 72, and 168 hours after laser photocoagulation and from unlasered eyes and were tested for VEGF, IL-1β, and TNF-α. Results. COX-2 null mice demonstrated 58% (P = 0.001) and 48% (P = 0.001) reductions in CNV formation on FA and choroidal flat mounts, respectively, compared with WT mice. For unlasered mice, mean VEGF concentrations in the retina and choroid were 1.2 ± 0.42 pg/mg protein for WT but only 0.42 ±0.2 pg/mg protein for COX-2 null mice (P < 0.05). After laser photocoagulation, WT mice showed significantly greater VEGF and IL-β expression in the retina and choroid by 168 hours (P < 0.05) and 72 hours (P < 0.05), respectively, compared with COX-2 null mice. Conclusions. COX-2 null mice exhibited significantly less choroidal neovascular membrane formation associated with reduced expression of VEGF. The results of this study suggest that COX-2 modulates VEGF expression in CNV and implicates a potential therapeutic role for nonsteroidal anti-inflammatory drugs.

AB - Purpose. To assess the degree of laser-induced choroidal neo-vascular membrane formation in wild-type (WT) and COX-2 null mice and to measure vascular endothelial growth factor (VEGF), interleukin (IL)-1, and tumor necrosis factor (TNF)-α levels in the retina and choroid. Methods. Four laser burns were placed in each eye of WT and COX-2 null mice to induce choroidal neovascularization. Fluorescein angiography (FA) was performed at 14 days, and retinal pigment epithelium-choroid-sclera (choroidal) flat mounts were prepared. The retina and choroid were isolated from WT and COX-2 null mice at 24, 72, and 168 hours after laser photocoagulation and from unlasered eyes and were tested for VEGF, IL-1β, and TNF-α. Results. COX-2 null mice demonstrated 58% (P = 0.001) and 48% (P = 0.001) reductions in CNV formation on FA and choroidal flat mounts, respectively, compared with WT mice. For unlasered mice, mean VEGF concentrations in the retina and choroid were 1.2 ± 0.42 pg/mg protein for WT but only 0.42 ±0.2 pg/mg protein for COX-2 null mice (P < 0.05). After laser photocoagulation, WT mice showed significantly greater VEGF and IL-β expression in the retina and choroid by 168 hours (P < 0.05) and 72 hours (P < 0.05), respectively, compared with COX-2 null mice. Conclusions. COX-2 null mice exhibited significantly less choroidal neovascular membrane formation associated with reduced expression of VEGF. The results of this study suggest that COX-2 modulates VEGF expression in CNV and implicates a potential therapeutic role for nonsteroidal anti-inflammatory drugs.

UR - http://www.scopus.com/inward/record.url?scp=79953275698&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79953275698&partnerID=8YFLogxK

U2 - 10.1167/iovs.10-6319

DO - 10.1167/iovs.10-6319

M3 - Article

C2 - 20881304

AN - SCOPUS:79953275698

VL - 52

SP - 701

EP - 707

JO - Investigative Ophthalmology and Visual Science

JF - Investigative Ophthalmology and Visual Science

SN - 0146-0404

IS - 2

ER -