Reduced virulence of an extensively drug-resistant outbreak strain of mycobacterium tuberculosis in a murine model

Kristen L.Jurcic Smith, Divey Saini, Svetoslav Bardarov, Michelle Larsen, Richard Frothingham, Neel R. Gandhi, William R. Jacobs, A. Willem Sturm, Sunhee Lee

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Bacterial drug resistance is often associated with a fitness cost. Large outbreaks of multidrug-resistant (MDR) and extensively drug-resistant (XDR) TB have been described that predominately affect persons with HIV infection. We obtained four closely-related Mycobacterium tuberculosis strains (genotype F15/LAM4/KZN) from an outbreak in KwaZulu-Natal (KZN), South Africa, including drug-sensitive, MDR, and XDR clinical isolates. We compared the virulence of these strains in a murine model of aerosol M. tuberculosis infection for four phenotypes: (1) competitive in vivo growth in lung and spleen, (2) non-competitive in vivo growth in lung and spleen, (3) murine survival time, and (4) lung pathology. When mixtures of sensitive, MDR, and XDR KZN strains were aerosolized (competitive model), lung CFUs were similar at 60 days after infection, and spleen CFUs were ordered as follows: sensitive > MDR > XDR. When individual strains were aerosolized (noncompetitive model), modest differences in lung and spleen CFUs were observed with the same ordering. C57BL/6, C3H/FeJ, and SCID mice all survived longer after infection with MDR as compared to sensitive strains. SCID mice infected with an XDR strain survived longer than those infected with MDR or sensitive strains. Lung pathology was reduced after XDR TB infection compared to sensitive or MDR TB infection. In summary, increasing degrees of drug resistance were associated with decreasing murine virulence in this collection of KZN strains as measured by all four virulence phenotypes. The predominance of HIV-infected patients in MDR and XDR TB outbreaks may be explained by decreased virulence of these strains in humans.

Original languageEnglish (US)
Article numbere94953
JournalPloS one
Volume9
Issue number4
DOIs
StatePublished - Apr 14 2014
Externally publishedYes

Fingerprint

Mycobacterium tuberculosis
Disease Outbreaks
Virulence
virulence
animal models
Extensively Drug-Resistant Tuberculosis
drugs
Lung
Spleen
lungs
Pharmaceutical Preparations
South Africa
SCID Mice
spleen
Infection
infection
mice
drug resistance
Bacterial Drug Resistance
Pathology

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Reduced virulence of an extensively drug-resistant outbreak strain of mycobacterium tuberculosis in a murine model. / Smith, Kristen L.Jurcic; Saini, Divey; Bardarov, Svetoslav; Larsen, Michelle; Frothingham, Richard; Gandhi, Neel R.; Jacobs, William R.; Sturm, A. Willem; Lee, Sunhee.

In: PloS one, Vol. 9, No. 4, e94953, 14.04.2014.

Research output: Contribution to journalArticle

Smith, KLJ, Saini, D, Bardarov, S, Larsen, M, Frothingham, R, Gandhi, NR, Jacobs, WR, Sturm, AW & Lee, S 2014, 'Reduced virulence of an extensively drug-resistant outbreak strain of mycobacterium tuberculosis in a murine model', PloS one, vol. 9, no. 4, e94953. https://doi.org/10.1371/journal.pone.0094953
Smith, Kristen L.Jurcic ; Saini, Divey ; Bardarov, Svetoslav ; Larsen, Michelle ; Frothingham, Richard ; Gandhi, Neel R. ; Jacobs, William R. ; Sturm, A. Willem ; Lee, Sunhee. / Reduced virulence of an extensively drug-resistant outbreak strain of mycobacterium tuberculosis in a murine model. In: PloS one. 2014 ; Vol. 9, No. 4.
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