Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice

Janna R. Jackson, Tyler J. Kirby, Christopher Fry, Robin L. Cooper, John J. McCarthy, Charlotte A. Peterson, Esther E. Dupont-Versteegden

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background: Satellite cells, or muscle stem cells, have been thought to be responsible for all muscle plasticity, but recent studies using genetically modified mouse models that allow for the conditional ablation of satellite cells have challenged this dogma. Results have confirmed the absolute requirement of satellite cells for muscle regeneration but surprisingly also showed that they are not required for adult muscle growth. While the function of satellite cells in muscle growth and regeneration is becoming better defined, their role in the response to aerobic activity remains largely unexplored. The purpose of the current study was to assess the involvement of satellite cells in response to aerobic exercise by evaluating the effect of satellite cell depletion on wheel running performance. Results: Four-month-old female Pax7/DTA mice (n = 8-12 per group) were satellite cell depleted via tamoxifen administration; at 6months of age, mice either remained sedentary or were provided with running wheels for 8weeks. Plantaris muscles were significantly depleted of Pax7+cells (≥90% depleted), and 8weeks of wheel running did not result in an increase in Pax7+ cells, or in myonuclear accretion. Interestingly, satellite cell-depleted animals ran ~27% less distance and were 23% slower than non-depleted animals. Wheel running was associated with elevated succinate dehydrogenase activity, muscle vascularization, lipid accumulation, and a significant shift toward more oxidative myosin heavy chain isoforms, as well as an increase in voltage dependent anion channel abundance, a marker of mitochondrial density. Importantly, these changes were independent of satellite cell content. Interestingly, depletion of Pax7+ cells from intra- as well as extrafusal muscle fibers resulted in atrophy of intrafusal fibers, thickening of muscle spindle-associated extracellular matrix, and a marked reduction of functional outcomes including grip strength, gait fluidity, and balance, which likely contributed to the impaired running performance. Conclusions: Depletion of Pax7-expressing cells in muscle resulted in reduced voluntary wheel running performance, without affecting markers of aerobic adaptation; however, their absence may perturb proprioception via disruption of muscle spindle fibers resulting in loss of gross motor coordination, indicating that satellite cells have a yet unexplored role in muscle function.

Original languageEnglish (US)
Article number41
JournalSkeletal Muscle
Volume5
Issue number1
DOIs
StatePublished - Nov 16 2015

Fingerprint

Running
Muscle Cells
Muscles
Muscle Spindles
Regeneration
Voltage-Dependent Anion Channels
Proprioception
Succinate Dehydrogenase
Myosin Heavy Chains
Hand Strength
Tamoxifen
Growth
Gait
Atrophy
Extracellular Matrix
Protein Isoforms
Skeletal Muscle
Stem Cells
Exercise
Lipids

Keywords

  • Aerobic capacity
  • Muscle spindles
  • Satellite cells
  • Wheel-running

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology
  • Orthopedics and Sports Medicine

Cite this

Jackson, J. R., Kirby, T. J., Fry, C., Cooper, R. L., McCarthy, J. J., Peterson, C. A., & Dupont-Versteegden, E. E. (2015). Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice. Skeletal Muscle, 5(1), [41]. https://doi.org/10.1186/s13395-015-0065-3

Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice. / Jackson, Janna R.; Kirby, Tyler J.; Fry, Christopher; Cooper, Robin L.; McCarthy, John J.; Peterson, Charlotte A.; Dupont-Versteegden, Esther E.

In: Skeletal Muscle, Vol. 5, No. 1, 41, 16.11.2015.

Research output: Contribution to journalArticle

Jackson, Janna R. ; Kirby, Tyler J. ; Fry, Christopher ; Cooper, Robin L. ; McCarthy, John J. ; Peterson, Charlotte A. ; Dupont-Versteegden, Esther E. / Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice. In: Skeletal Muscle. 2015 ; Vol. 5, No. 1.
@article{f3c95835f3e545e89736be029cdaa40c,
title = "Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice",
abstract = "Background: Satellite cells, or muscle stem cells, have been thought to be responsible for all muscle plasticity, but recent studies using genetically modified mouse models that allow for the conditional ablation of satellite cells have challenged this dogma. Results have confirmed the absolute requirement of satellite cells for muscle regeneration but surprisingly also showed that they are not required for adult muscle growth. While the function of satellite cells in muscle growth and regeneration is becoming better defined, their role in the response to aerobic activity remains largely unexplored. The purpose of the current study was to assess the involvement of satellite cells in response to aerobic exercise by evaluating the effect of satellite cell depletion on wheel running performance. Results: Four-month-old female Pax7/DTA mice (n = 8-12 per group) were satellite cell depleted via tamoxifen administration; at 6months of age, mice either remained sedentary or were provided with running wheels for 8weeks. Plantaris muscles were significantly depleted of Pax7+cells (≥90{\%} depleted), and 8weeks of wheel running did not result in an increase in Pax7+ cells, or in myonuclear accretion. Interestingly, satellite cell-depleted animals ran ~27{\%} less distance and were 23{\%} slower than non-depleted animals. Wheel running was associated with elevated succinate dehydrogenase activity, muscle vascularization, lipid accumulation, and a significant shift toward more oxidative myosin heavy chain isoforms, as well as an increase in voltage dependent anion channel abundance, a marker of mitochondrial density. Importantly, these changes were independent of satellite cell content. Interestingly, depletion of Pax7+ cells from intra- as well as extrafusal muscle fibers resulted in atrophy of intrafusal fibers, thickening of muscle spindle-associated extracellular matrix, and a marked reduction of functional outcomes including grip strength, gait fluidity, and balance, which likely contributed to the impaired running performance. Conclusions: Depletion of Pax7-expressing cells in muscle resulted in reduced voluntary wheel running performance, without affecting markers of aerobic adaptation; however, their absence may perturb proprioception via disruption of muscle spindle fibers resulting in loss of gross motor coordination, indicating that satellite cells have a yet unexplored role in muscle function.",
keywords = "Aerobic capacity, Muscle spindles, Satellite cells, Wheel-running",
author = "Jackson, {Janna R.} and Kirby, {Tyler J.} and Christopher Fry and Cooper, {Robin L.} and McCarthy, {John J.} and Peterson, {Charlotte A.} and Dupont-Versteegden, {Esther E.}",
year = "2015",
month = "11",
day = "16",
doi = "10.1186/s13395-015-0065-3",
language = "English (US)",
volume = "5",
journal = "Skeletal Muscle",
issn = "2044-5040",
publisher = "BioMed Central",
number = "1",

}

TY - JOUR

T1 - Reduced voluntary running performance is associated with impaired coordination as a result of muscle satellite cell depletion in adult mice

AU - Jackson, Janna R.

AU - Kirby, Tyler J.

AU - Fry, Christopher

AU - Cooper, Robin L.

AU - McCarthy, John J.

AU - Peterson, Charlotte A.

AU - Dupont-Versteegden, Esther E.

PY - 2015/11/16

Y1 - 2015/11/16

N2 - Background: Satellite cells, or muscle stem cells, have been thought to be responsible for all muscle plasticity, but recent studies using genetically modified mouse models that allow for the conditional ablation of satellite cells have challenged this dogma. Results have confirmed the absolute requirement of satellite cells for muscle regeneration but surprisingly also showed that they are not required for adult muscle growth. While the function of satellite cells in muscle growth and regeneration is becoming better defined, their role in the response to aerobic activity remains largely unexplored. The purpose of the current study was to assess the involvement of satellite cells in response to aerobic exercise by evaluating the effect of satellite cell depletion on wheel running performance. Results: Four-month-old female Pax7/DTA mice (n = 8-12 per group) were satellite cell depleted via tamoxifen administration; at 6months of age, mice either remained sedentary or were provided with running wheels for 8weeks. Plantaris muscles were significantly depleted of Pax7+cells (≥90% depleted), and 8weeks of wheel running did not result in an increase in Pax7+ cells, or in myonuclear accretion. Interestingly, satellite cell-depleted animals ran ~27% less distance and were 23% slower than non-depleted animals. Wheel running was associated with elevated succinate dehydrogenase activity, muscle vascularization, lipid accumulation, and a significant shift toward more oxidative myosin heavy chain isoforms, as well as an increase in voltage dependent anion channel abundance, a marker of mitochondrial density. Importantly, these changes were independent of satellite cell content. Interestingly, depletion of Pax7+ cells from intra- as well as extrafusal muscle fibers resulted in atrophy of intrafusal fibers, thickening of muscle spindle-associated extracellular matrix, and a marked reduction of functional outcomes including grip strength, gait fluidity, and balance, which likely contributed to the impaired running performance. Conclusions: Depletion of Pax7-expressing cells in muscle resulted in reduced voluntary wheel running performance, without affecting markers of aerobic adaptation; however, their absence may perturb proprioception via disruption of muscle spindle fibers resulting in loss of gross motor coordination, indicating that satellite cells have a yet unexplored role in muscle function.

AB - Background: Satellite cells, or muscle stem cells, have been thought to be responsible for all muscle plasticity, but recent studies using genetically modified mouse models that allow for the conditional ablation of satellite cells have challenged this dogma. Results have confirmed the absolute requirement of satellite cells for muscle regeneration but surprisingly also showed that they are not required for adult muscle growth. While the function of satellite cells in muscle growth and regeneration is becoming better defined, their role in the response to aerobic activity remains largely unexplored. The purpose of the current study was to assess the involvement of satellite cells in response to aerobic exercise by evaluating the effect of satellite cell depletion on wheel running performance. Results: Four-month-old female Pax7/DTA mice (n = 8-12 per group) were satellite cell depleted via tamoxifen administration; at 6months of age, mice either remained sedentary or were provided with running wheels for 8weeks. Plantaris muscles were significantly depleted of Pax7+cells (≥90% depleted), and 8weeks of wheel running did not result in an increase in Pax7+ cells, or in myonuclear accretion. Interestingly, satellite cell-depleted animals ran ~27% less distance and were 23% slower than non-depleted animals. Wheel running was associated with elevated succinate dehydrogenase activity, muscle vascularization, lipid accumulation, and a significant shift toward more oxidative myosin heavy chain isoforms, as well as an increase in voltage dependent anion channel abundance, a marker of mitochondrial density. Importantly, these changes were independent of satellite cell content. Interestingly, depletion of Pax7+ cells from intra- as well as extrafusal muscle fibers resulted in atrophy of intrafusal fibers, thickening of muscle spindle-associated extracellular matrix, and a marked reduction of functional outcomes including grip strength, gait fluidity, and balance, which likely contributed to the impaired running performance. Conclusions: Depletion of Pax7-expressing cells in muscle resulted in reduced voluntary wheel running performance, without affecting markers of aerobic adaptation; however, their absence may perturb proprioception via disruption of muscle spindle fibers resulting in loss of gross motor coordination, indicating that satellite cells have a yet unexplored role in muscle function.

KW - Aerobic capacity

KW - Muscle spindles

KW - Satellite cells

KW - Wheel-running

UR - http://www.scopus.com/inward/record.url?scp=84960125449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84960125449&partnerID=8YFLogxK

U2 - 10.1186/s13395-015-0065-3

DO - 10.1186/s13395-015-0065-3

M3 - Article

AN - SCOPUS:84960125449

VL - 5

JO - Skeletal Muscle

JF - Skeletal Muscle

SN - 2044-5040

IS - 1

M1 - 41

ER -