Reductant and energy level of bacteroids of Rhizobium leguminosarum

Nisha Garg; H. S. Nainawatee

doi:10.1016/0031-9422(89)80252-3

Reductant and energy level of bacteroids of Rhizobium leguminosarum

Nisha Garg, H. S. Nainawatee

Research output: Contribution to journal › Article › peer-review

Abstract

Low redox potential electron carriers such as benzyl viologen and NAD⁺ were reduced by bacteroids prepared from nodules of pea inoculated with hup^{+ R. leguminosarum} strain Vp1, whereas, the bacteroids of plasmid cured hup^- derivative Vm1 did not exhibit the reducing activity. The bacteroids prepared from nodules of hup⁺ strain Vp1 at 46d stage, where nitrogenase activity was maximum, exhibited 1.5-fold higher levels of NADH/NADPH, two-fold higher levels of ATP while the level of ADP and AMP were lower as compared to the bacteroids of hup^- strain Vm1. Also the level of adenylate energy charge in ^hup+ bacteroids was 29% higher than the hup^- bacteroids.

Original language	English (US)
Pages (from-to)	2913-2915
Number of pages	3
Journal	Phytochemistry
Volume	28
Issue number	11
DOIs	https://doi.org/10.1016/0031-9422(89)80252-3
State	Published - 1989
Externally published	Yes

Keywords

Cyanophyceae
Rhizobium leguminosarum
adenylate charge
bacteroids
hydrogen uptake.

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Plant Science
Horticulture

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10.1016/0031-9422(89)80252-3

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title = "Reductant and energy level of bacteroids of Rhizobium leguminosarum",

abstract = "Low redox potential electron carriers such as benzyl viologen and NAD+ were reduced by bacteroids prepared from nodules of pea inoculated with hup+ R. leguminosarum strain Vp1, whereas, the bacteroids of plasmid cured hup- derivative Vm1 did not exhibit the reducing activity. The bacteroids prepared from nodules of hup+ strain Vp1 at 46d stage, where nitrogenase activity was maximum, exhibited 1.5-fold higher levels of NADH/NADPH, two-fold higher levels of ATP while the level of ADP and AMP were lower as compared to the bacteroids of hup- strain Vm1. Also the level of adenylate energy charge in hup+ bacteroids was 29% higher than the hup- bacteroids.",

keywords = "Cyanophyceae, Rhizobium leguminosarum, adenylate charge, bacteroids, hydrogen uptake.",

author = "Nisha Garg and Nainawatee, {H. S.}",

note = "Funding Information: Acknowledgements-NG acknowledges with thanks support from The Council of Scientific and Industrial Research, New Delhi for the award of a Senior Research Fellowship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1989",

doi = "10.1016/0031-9422(89)80252-3",

language = "English (US)",

volume = "28",

pages = "2913--2915",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Limited",

number = "11",

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TY - JOUR

T1 - Reductant and energy level of bacteroids of Rhizobium leguminosarum

AU - Garg, Nisha

AU - Nainawatee, H. S.

N1 - Funding Information: Acknowledgements-NG acknowledges with thanks support from The Council of Scientific and Industrial Research, New Delhi for the award of a Senior Research Fellowship. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1989

Y1 - 1989

N2 - Low redox potential electron carriers such as benzyl viologen and NAD+ were reduced by bacteroids prepared from nodules of pea inoculated with hup+ R. leguminosarum strain Vp1, whereas, the bacteroids of plasmid cured hup- derivative Vm1 did not exhibit the reducing activity. The bacteroids prepared from nodules of hup+ strain Vp1 at 46d stage, where nitrogenase activity was maximum, exhibited 1.5-fold higher levels of NADH/NADPH, two-fold higher levels of ATP while the level of ADP and AMP were lower as compared to the bacteroids of hup- strain Vm1. Also the level of adenylate energy charge in hup+ bacteroids was 29% higher than the hup- bacteroids.

AB - Low redox potential electron carriers such as benzyl viologen and NAD+ were reduced by bacteroids prepared from nodules of pea inoculated with hup+ R. leguminosarum strain Vp1, whereas, the bacteroids of plasmid cured hup- derivative Vm1 did not exhibit the reducing activity. The bacteroids prepared from nodules of hup+ strain Vp1 at 46d stage, where nitrogenase activity was maximum, exhibited 1.5-fold higher levels of NADH/NADPH, two-fold higher levels of ATP while the level of ADP and AMP were lower as compared to the bacteroids of hup- strain Vm1. Also the level of adenylate energy charge in hup+ bacteroids was 29% higher than the hup- bacteroids.

KW - Cyanophyceae

KW - Rhizobium leguminosarum

KW - adenylate charge

KW - bacteroids

KW - hydrogen uptake.

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U2 - 10.1016/0031-9422(89)80252-3

DO - 10.1016/0031-9422(89)80252-3

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SN - 0031-9422

VL - 28

SP - 2913

EP - 2915

JO - Phytochemistry

JF - Phytochemistry

IS - 11

ER -

Reductant and energy level of bacteroids of Rhizobium leguminosarum

Abstract

Keywords

ASJC Scopus subject areas

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