Reduction of 4-hydroxynonenal and 4-hydroxyhexenal by retinal aldose reductase

Qin He, Peeyush Khanna, Sanjay Srivastava, Frederik J G M Van Kuijk, Naseem Ansari

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Aldose reductase has been purified to homogeneity from bovine retina. It has an apparent molecular weight of 32,000 daltons and shares immunological and kinetic properties with the much studied aldose reductases purified from various sources. Retinal aldose reductase displays a K(m) of approximately 40 μM with 4-hydroxynonenal and 4-hydroxyhexenal, the oxidation end products of arachidonic and docosahexanoeic acids, respectively. It therefore appears that aldose reductase may constitute a major detoxification route of these toxic aldehydes in the retina.

Original languageEnglish (US)
Pages (from-to)719-722
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number3
DOIs
StatePublished - Jun 29 1998

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Aldehyde Reductase
Retina
Arachidonic Acids
Detoxification
Poisons
Aldehydes
Molecular Weight
Molecular weight
Oxidation
Kinetics
4-hydroxyhexenal
4-hydroxy-2-nonenal
alcohol dehydrogenase (NAD(P)+)

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Reduction of 4-hydroxynonenal and 4-hydroxyhexenal by retinal aldose reductase. / He, Qin; Khanna, Peeyush; Srivastava, Sanjay; Van Kuijk, Frederik J G M; Ansari, Naseem.

In: Biochemical and Biophysical Research Communications, Vol. 247, No. 3, 29.06.1998, p. 719-722.

Research output: Contribution to journalArticle

He, Qin ; Khanna, Peeyush ; Srivastava, Sanjay ; Van Kuijk, Frederik J G M ; Ansari, Naseem. / Reduction of 4-hydroxynonenal and 4-hydroxyhexenal by retinal aldose reductase. In: Biochemical and Biophysical Research Communications. 1998 ; Vol. 247, No. 3. pp. 719-722.
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