Reduction of 4-hydroxynonenal and 4-hydroxyhexenal by retinal aldose reductase

Qin He, Peeyush Khanna, Sanjay Srivastava, Frederik J.G.M. Van Kuijk, Naseem H. Ansari

Research output: Contribution to journalArticlepeer-review

31 Scopus citations

Abstract

Aldose reductase has been purified to homogeneity from bovine retina. It has an apparent molecular weight of 32,000 daltons and shares immunological and kinetic properties with the much studied aldose reductases purified from various sources. Retinal aldose reductase displays a K(m) of approximately 40 μM with 4-hydroxynonenal and 4-hydroxyhexenal, the oxidation end products of arachidonic and docosahexanoeic acids, respectively. It therefore appears that aldose reductase may constitute a major detoxification route of these toxic aldehydes in the retina.

Original languageEnglish (US)
Pages (from-to)719-722
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume247
Issue number3
DOIs
StatePublished - Jun 29 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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