TY - JOUR
T1 - Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice
AU - Guerrero, Thomas
AU - Castillo, Richard
AU - Noyola-Martinez, Josue
AU - Torres, Mylin
AU - Zhou, Xinhui
AU - Guerra, Rudy
AU - Cody, Dianna
AU - Komaki, Ritsuko
AU - Travis, Elizabeth
PY - 2007/3/1
Y1 - 2007/3/1
N2 - Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice. Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue. Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (± 11) Hounsfield units (HU) to -378 (± 11) HU. For the control mice, the mean CT values ranged from -549 (± 11) HU to -633 (± 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [± 0.0038] vs. 0.106 [± 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures. Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury.
AB - Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice. Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue. Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (± 11) Hounsfield units (HU) to -378 (± 11) HU. For the control mice, the mean CT values ranged from -549 (± 11) HU to -633 (± 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [± 0.0038] vs. 0.106 [± 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures. Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury.
KW - Computed tomography
KW - Mouse model
KW - Pulmonary injury
KW - Thoracic radiation
UR - http://www.scopus.com/inward/record.url?scp=33846827350&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33846827350&partnerID=8YFLogxK
U2 - 10.1016/j.ijrobp.2006.10.037
DO - 10.1016/j.ijrobp.2006.10.037
M3 - Article
C2 - 17293238
AN - SCOPUS:33846827350
SN - 0360-3016
VL - 67
SP - 879
EP - 887
JO - International Journal of Radiation Oncology Biology Physics
JF - International Journal of Radiation Oncology Biology Physics
IS - 3
ER -