Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice

Thomas Guerrero, Richard Castillo, Josue Noyola-Martinez, Mylin Torres, Xinhui Zhou, Rudy Guerra, Dianna Cody, Ritsuko Komaki, Elizabeth Travis

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice. Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue. Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (± 11) Hounsfield units (HU) to -378 (± 11) HU. For the control mice, the mean CT values ranged from -549 (± 11) HU to -633 (± 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [± 0.0038] vs. 0.106 [± 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures. Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury.

Original languageEnglish (US)
Pages (from-to)879-887
Number of pages9
JournalInternational Journal of Radiation Oncology Biology Physics
Volume67
Issue number3
DOIs
StatePublished - Mar 1 2007
Externally publishedYes

Fingerprint

Lung Compliance
mice
tomography
Tomography
lungs
high resolution
Compliance
Pressure
Lung
animals
Air
radiation injuries
Radiation Injuries
irradiation
air
Lung Injury
confidence
Thorax
Confidence Intervals
intervals

Keywords

  • Computed tomography
  • Mouse model
  • Pulmonary injury
  • Thoracic radiation

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Radiation

Cite this

Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice. / Guerrero, Thomas; Castillo, Richard; Noyola-Martinez, Josue; Torres, Mylin; Zhou, Xinhui; Guerra, Rudy; Cody, Dianna; Komaki, Ritsuko; Travis, Elizabeth.

In: International Journal of Radiation Oncology Biology Physics, Vol. 67, No. 3, 01.03.2007, p. 879-887.

Research output: Contribution to journalArticle

Guerrero, T, Castillo, R, Noyola-Martinez, J, Torres, M, Zhou, X, Guerra, R, Cody, D, Komaki, R & Travis, E 2007, 'Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice', International Journal of Radiation Oncology Biology Physics, vol. 67, no. 3, pp. 879-887. https://doi.org/10.1016/j.ijrobp.2006.10.037
Guerrero, Thomas ; Castillo, Richard ; Noyola-Martinez, Josue ; Torres, Mylin ; Zhou, Xinhui ; Guerra, Rudy ; Cody, Dianna ; Komaki, Ritsuko ; Travis, Elizabeth. / Reduction of pulmonary compliance found with high-resolution computed tomography in irradiated mice. In: International Journal of Radiation Oncology Biology Physics. 2007 ; Vol. 67, No. 3. pp. 879-887.
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AU - Guerrero, Thomas

AU - Castillo, Richard

AU - Noyola-Martinez, Josue

AU - Torres, Mylin

AU - Zhou, Xinhui

AU - Guerra, Rudy

AU - Cody, Dianna

AU - Komaki, Ritsuko

AU - Travis, Elizabeth

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N2 - Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice. Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue. Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (± 11) Hounsfield units (HU) to -378 (± 11) HU. For the control mice, the mean CT values ranged from -549 (± 11) HU to -633 (± 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [± 0.0038] vs. 0.106 [± 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures. Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury.

AB - Purpose: To demonstrate that high-resolution computed tomography (CT) can be used to quantify loss of pulmonary compliance in irradiated mice. Methods and Materials: Computed tomography images of three nonirradiated (controls) and three irradiated mice were obtained 200 days after a single dose of 16-Gy Co (60) thoracic irradiation. While intubated, each animal was imaged at static breath-hold pressures of 2, 10, and 18 cm H2O. A deformable image registration algorithm was used to calculate changes in air volume between adjacent-pressure CT image pairs (e.g., 2 and 10 cm H2O), and functional images of pulmonary compliance were generated. The mass-specific compliance was calculated as the change in volume divided by the pressure difference between the 2 image sets and the mass of lung tissue. Results: For the irradiated mice, the lung parenchyma mean CT values ranged from -314 (± 11) Hounsfield units (HU) to -378 (± 11) HU. For the control mice, the mean CT values ranged from -549 (± 11) HU to -633 (± 11) HU. Irradiated mice had a 60% (45, 74%; 95% confidence interval) lower mass-specific compliance than did the controls (0.039 [± 0.0038] vs. 0.106 [± 0.0038] mL air per cm H2O per g lung) from the 2-cm to 10-cm H2O CT image pair. The difference in compliance between groups was less pronounced at the higher distending pressures. Conclusion: High-resolution CT was used to quantify a reduction in mass-specific compliance following whole lung irradiation in mice. This small animal radiation injury model and assay may be useful in the study of lung injury.

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KW - Mouse model

KW - Pulmonary injury

KW - Thoracic radiation

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