Abstract
Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1279-1283 |
| Number of pages | 5 |
| Journal | Microbiology |
| Volume | 158 |
| Issue number | 5 |
| DOIs | |
| State | Published - May 2012 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Microbiology
Cite this
Reduction of the monounsaturated fatty acid content of Escherichia coli results in increased resistance to oxidative damage. / Pradenas, Gonzalo A.; Paillavil, Braulio A.; Reyes-Cerpa, Sebastián; Pérez-Donoso, José M.; Vásquez, Claudio C.
In: Microbiology, Vol. 158, No. 5, 05.2012, p. 1279-1283.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Reduction of the monounsaturated fatty acid content of Escherichia coli results in increased resistance to oxidative damage
AU - Pradenas, Gonzalo A.
AU - Paillavil, Braulio A.
AU - Reyes-Cerpa, Sebastián
AU - Pérez-Donoso, José M.
AU - Vásquez, Claudio C.
PY - 2012/5
Y1 - 2012/5
N2 - Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.
AB - Reactive oxygen species (ROSs) affect several macromolecules and cellular components in eukaryotic and prokaryotic cells. In this work, the effect of various ROS-generating compounds on the Escherichia coli membrane was studied. Membrane fatty acid profiles, oxidative damage levels and bacterial resistance to these toxicants were determined. Studies included wild-type cells as well as a strain exhibiting a modified monounsaturated fatty acid (MUFA) profile (accomplished by overexpressing the β-hydroxyacyl acyl carrier protein dehydratase-encoding gene, fabA). Levels of membrane MUFAs and oxidative damage markers decreased slightly upon toxicant exposure with a concomitant increase in cell resistance to these ROS-generating compounds. A direct relationship between MUFAs and lipid peroxidation was observed. The lower the MUFA the lower the peroxide levels, suggesting that MUFAs are targets for membrane lipid oxidation.
UR - http://www.scopus.com/inward/record.url?scp=84860538768&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84860538768&partnerID=8YFLogxK
U2 - 10.1099/mic.0.056903-0
DO - 10.1099/mic.0.056903-0
M3 - Article
C2 - 22343353
AN - SCOPUS:84860538768
VL - 158
SP - 1279
EP - 1283
JO - Microbiology (United Kingdom)
JF - Microbiology (United Kingdom)
SN - 1350-0872
IS - 5
ER -