Refined atomic model of the four-layer aggregate of the tobacco mosaic virus coat protein at 2.4-Å resolution

Balaji Bhyravbhatla, Stanley Watowich, Donald L D Caspar

Research output: Contribution to journalArticle

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Abstract

Previous x-ray studies (2.8-Å resolution) on crystals of tobacco mosaic virus coat protein grown from solutions containing high salt have characterized the structure of the protein aggregate as a dimer of a bilayered cylindrical disk formed by 34 chemically identical subunits. We have determined the crystal structure of the disk aggregate at 2.4-Å resolution using x-ray diffraction from crystals maintained at cryogenic temperatures. Two regions of interest have been extensively refined. First, residues of the low-radius loop region, which were not modeled previously, have been traced completely in our electron density maps. Similar to the structure observed in the virus, the right radial helix in each protomer ends around residue 87, after which the protein chain forms an extended chain that extends to the left radial helix. The left radial helix appears as a long α- helix with high temperature factors for the main-chain atoms in the inner portion. The side-chain atoms in this region (residues 90-110) are not visible in the electron density maps and are assumed to be disordered. Second, interactions between subunits in the symmetry-related central A pair have been determined. No direct protein-protein interactions are observed in the major overlap region between these subunits; all interactions are mediated by two layers of ordered solvent molecules. The current structure emphasizes the importance of water in biological macromolecular assemblies.

Original languageEnglish (US)
Pages (from-to)604-615
Number of pages12
JournalBiophysical Journal
Volume74
Issue number1
StatePublished - Jan 1998

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Tobacco Mosaic Virus
Capsid Proteins
X-Rays
Electrons
Proteins
Temperature
Protein Subunits
Salts
Viruses
Water

ASJC Scopus subject areas

  • Biophysics

Cite this

Refined atomic model of the four-layer aggregate of the tobacco mosaic virus coat protein at 2.4-Å resolution. / Bhyravbhatla, Balaji; Watowich, Stanley; Caspar, Donald L D.

In: Biophysical Journal, Vol. 74, No. 1, 01.1998, p. 604-615.

Research output: Contribution to journalArticle

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