Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat

Ping Wu, Yevgeniya I. Tarasenko, Yanping Gu, Li-Yen Huang, Richard E. Coggeshall, Yongjia Yu

Research output: Contribution to journalArticle

185 Citations (Scopus)

Abstract

Pluripotent or multipotent stem cells isolated from human embryos or adult central nervous system (CNS) may provide new neurons to ameliorate neural disorders. A major obstacle, however, is that the majority of such cells do not differentiate into neurons when grafted into non-neurogenic areas of the adult CNS. Here we report a new in vitro priming procedure that generates a nearly pure population of neurons from fetal human neural stem cells (hNSCs) transplanted into adult rat CNS. Furthermore, the grafted cells differentiated by acquiring a cholinergic phenotype in a region-specific manner. This technology may advance stem cell-based therapy to replace lost neurons in neural injury or neurodegenerative disorders.

Original languageEnglish (US)
Pages (from-to)1271-1278
Number of pages8
JournalNature Neuroscience
Volume5
Issue number12
DOIs
StatePublished - Dec 1 2002

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Cholinergic Neurons
Neural Stem Cells
Neurons
Central Nervous System
Multipotent Stem Cells
Pluripotent Stem Cells
Cell- and Tissue-Based Therapy
Neurodegenerative Diseases
Cholinergic Agents
Stem Cells
Embryonic Structures
Technology
Phenotype
Wounds and Injuries
Population

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Region-specific generation of cholinergic neurons from fetal human neural stem cells grafted in adult rat. / Wu, Ping; Tarasenko, Yevgeniya I.; Gu, Yanping; Huang, Li-Yen; Coggeshall, Richard E.; Yu, Yongjia.

In: Nature Neuroscience, Vol. 5, No. 12, 01.12.2002, p. 1271-1278.

Research output: Contribution to journalArticle

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