Regional acetate kinetics and oxidation in human volunteers

B. Mittendorfer, L. S. Sidossis, Eric Walser, D. L. Chinkes, R. R. Wolfe

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

We have used a 3-h primed continuous infusion of [1,2-13C]acetate in five fasted (24 h) volunteers to quantify splanchnic and leg acetate metabolism (protocol 1). Fractional extraction of acetate by both tissues was high (~70%), and simultaneous uptake and release of acetate were observed. Labeled carbon recovery in CO2 was 37.9 ± 2.3% at the whole body level, 37.7 ± 1.5% across the splanchnic bed, and 37.3 ± 2.9% across the leg. Furthermore, we calculated whole body labeled carbon recovery during 15 h of [1,2-13C]acetate infusion in three volunteers (protocol 2). Whole body acetate carbon recovery in CO2 was significantly higher (66.7 ± 4.5%) after 15 h of tracer infusion than after 3 h. We conclude that acetate is rapidly taken up by the leg and splanchnic tissues and that the percent recovery of CO2 from the oxidation of acetate is heavily dependent on the length of acetate tracer infusion. In the postabsorptive state, labeled carbon recovery from acetate across the leg and the splanchnic region is similar to the whole body CO2 recovery.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume274
Issue number6 37-6
StatePublished - Jun 1998

Fingerprint

Volunteers
Acetates
Oxidation
Kinetics
Viscera
Recovery
Leg
Carbon
Tissue
Metabolism

Keywords

  • Acetate correction factor
  • Fatty acids
  • Liver
  • Muscle

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry

Cite this

Mittendorfer, B., Sidossis, L. S., Walser, E., Chinkes, D. L., & Wolfe, R. R. (1998). Regional acetate kinetics and oxidation in human volunteers. American Journal of Physiology - Endocrinology and Metabolism, 274(6 37-6).

Regional acetate kinetics and oxidation in human volunteers. / Mittendorfer, B.; Sidossis, L. S.; Walser, Eric; Chinkes, D. L.; Wolfe, R. R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 274, No. 6 37-6, 06.1998.

Research output: Contribution to journalArticle

Mittendorfer, B, Sidossis, LS, Walser, E, Chinkes, DL & Wolfe, RR 1998, 'Regional acetate kinetics and oxidation in human volunteers', American Journal of Physiology - Endocrinology and Metabolism, vol. 274, no. 6 37-6.
Mittendorfer, B. ; Sidossis, L. S. ; Walser, Eric ; Chinkes, D. L. ; Wolfe, R. R. / Regional acetate kinetics and oxidation in human volunteers. In: American Journal of Physiology - Endocrinology and Metabolism. 1998 ; Vol. 274, No. 6 37-6.
@article{56f01da3161446eea70b1cf320192d9d,
title = "Regional acetate kinetics and oxidation in human volunteers",
abstract = "We have used a 3-h primed continuous infusion of [1,2-13C]acetate in five fasted (24 h) volunteers to quantify splanchnic and leg acetate metabolism (protocol 1). Fractional extraction of acetate by both tissues was high (~70{\%}), and simultaneous uptake and release of acetate were observed. Labeled carbon recovery in CO2 was 37.9 ± 2.3{\%} at the whole body level, 37.7 ± 1.5{\%} across the splanchnic bed, and 37.3 ± 2.9{\%} across the leg. Furthermore, we calculated whole body labeled carbon recovery during 15 h of [1,2-13C]acetate infusion in three volunteers (protocol 2). Whole body acetate carbon recovery in CO2 was significantly higher (66.7 ± 4.5{\%}) after 15 h of tracer infusion than after 3 h. We conclude that acetate is rapidly taken up by the leg and splanchnic tissues and that the percent recovery of CO2 from the oxidation of acetate is heavily dependent on the length of acetate tracer infusion. In the postabsorptive state, labeled carbon recovery from acetate across the leg and the splanchnic region is similar to the whole body CO2 recovery.",
keywords = "Acetate correction factor, Fatty acids, Liver, Muscle",
author = "B. Mittendorfer and Sidossis, {L. S.} and Eric Walser and Chinkes, {D. L.} and Wolfe, {R. R.}",
year = "1998",
month = "6",
language = "English (US)",
volume = "274",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "6 37-6",

}

TY - JOUR

T1 - Regional acetate kinetics and oxidation in human volunteers

AU - Mittendorfer, B.

AU - Sidossis, L. S.

AU - Walser, Eric

AU - Chinkes, D. L.

AU - Wolfe, R. R.

PY - 1998/6

Y1 - 1998/6

N2 - We have used a 3-h primed continuous infusion of [1,2-13C]acetate in five fasted (24 h) volunteers to quantify splanchnic and leg acetate metabolism (protocol 1). Fractional extraction of acetate by both tissues was high (~70%), and simultaneous uptake and release of acetate were observed. Labeled carbon recovery in CO2 was 37.9 ± 2.3% at the whole body level, 37.7 ± 1.5% across the splanchnic bed, and 37.3 ± 2.9% across the leg. Furthermore, we calculated whole body labeled carbon recovery during 15 h of [1,2-13C]acetate infusion in three volunteers (protocol 2). Whole body acetate carbon recovery in CO2 was significantly higher (66.7 ± 4.5%) after 15 h of tracer infusion than after 3 h. We conclude that acetate is rapidly taken up by the leg and splanchnic tissues and that the percent recovery of CO2 from the oxidation of acetate is heavily dependent on the length of acetate tracer infusion. In the postabsorptive state, labeled carbon recovery from acetate across the leg and the splanchnic region is similar to the whole body CO2 recovery.

AB - We have used a 3-h primed continuous infusion of [1,2-13C]acetate in five fasted (24 h) volunteers to quantify splanchnic and leg acetate metabolism (protocol 1). Fractional extraction of acetate by both tissues was high (~70%), and simultaneous uptake and release of acetate were observed. Labeled carbon recovery in CO2 was 37.9 ± 2.3% at the whole body level, 37.7 ± 1.5% across the splanchnic bed, and 37.3 ± 2.9% across the leg. Furthermore, we calculated whole body labeled carbon recovery during 15 h of [1,2-13C]acetate infusion in three volunteers (protocol 2). Whole body acetate carbon recovery in CO2 was significantly higher (66.7 ± 4.5%) after 15 h of tracer infusion than after 3 h. We conclude that acetate is rapidly taken up by the leg and splanchnic tissues and that the percent recovery of CO2 from the oxidation of acetate is heavily dependent on the length of acetate tracer infusion. In the postabsorptive state, labeled carbon recovery from acetate across the leg and the splanchnic region is similar to the whole body CO2 recovery.

KW - Acetate correction factor

KW - Fatty acids

KW - Liver

KW - Muscle

UR - http://www.scopus.com/inward/record.url?scp=0031859472&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031859472&partnerID=8YFLogxK

M3 - Article

C2 - 9611145

AN - SCOPUS:0031859472

VL - 274

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 6 37-6

ER -