Regional differences in L-selectin expression in murine intestinal lymphocytes

F. Seibold, B. Seibold-Schmid, Y. Cong, Yu Shu Feng Yu Shu, R. P. McCabe, C. Weaver, C. O. Elson

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Background and Aims: The expression of the lymphocyte homing receptor and activation marker L-selectin is different in colon and small intestinal intraepithelial lymphocytes (IELs). In this study, the mechanism of this difference in L-selectin expression was investigated. Methods: L-selectin expression on lymphocytes was measured by flow cytometry. L-selectin messenger RNA (mRNA) was detected by reverse-transcription polymerase chain reaction. L-Selectin expression on peripheral lymphocytes was analyzed after incubation with cytokines, food and bacterial antigens, and homogenates of small and large bowel. Results: L-selectin was expressed by none of the small intestinal IELs but by 30% of those in the colon and by 60% of splenocytes. mRNA for L-selectin was detectable in isolated lymphocytes of all three sites. L-Selectin was downregulated in colon IELs during colitis and up- regulated in small intestinal IELs after in vitro culture for 48 hours. Incubation of splenocytes with small intestinal homogenates led to a rapid down-regulation of L-selectin (1% vs. 60% untreated). Preincubation with a metalloproteinase inhibitor prevented L-selectin loss. Conclusions: The mechanism of the differential expression of L-selectin in mouse small intestine and colon appears to be an increased functional activity of a metalloproteinase (sheddase) in the small intestine compared with the colon.

Original languageEnglish (US)
Pages (from-to)965-974
Number of pages10
JournalGastroenterology
Volume114
Issue number5
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology

Fingerprint Dive into the research topics of 'Regional differences in L-selectin expression in murine intestinal lymphocytes'. Together they form a unique fingerprint.

  • Cite this

    Seibold, F., Seibold-Schmid, B., Cong, Y., Feng Yu Shu, Y. S., McCabe, R. P., Weaver, C., & Elson, C. O. (1998). Regional differences in L-selectin expression in murine intestinal lymphocytes. Gastroenterology, 114(5), 965-974. https://doi.org/10.1016/S0016-5085(98)70316-6