Regional differences in L-selectin expression in murine intestinal lymphocytes

F. Seibold, B. Seibold-Schmid, Yingzi Cong, Yu Shu Feng Yu Shu, R. P. McCabe, C. Weaver, C. O. Elson

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Background and Aims: The expression of the lymphocyte homing receptor and activation marker L-selectin is different in colon and small intestinal intraepithelial lymphocytes (IELs). In this study, the mechanism of this difference in L-selectin expression was investigated. Methods: L-selectin expression on lymphocytes was measured by flow cytometry. L-selectin messenger RNA (mRNA) was detected by reverse-transcription polymerase chain reaction. L-Selectin expression on peripheral lymphocytes was analyzed after incubation with cytokines, food and bacterial antigens, and homogenates of small and large bowel. Results: L-selectin was expressed by none of the small intestinal IELs but by 30% of those in the colon and by 60% of splenocytes. mRNA for L-selectin was detectable in isolated lymphocytes of all three sites. L-Selectin was downregulated in colon IELs during colitis and up- regulated in small intestinal IELs after in vitro culture for 48 hours. Incubation of splenocytes with small intestinal homogenates led to a rapid down-regulation of L-selectin (1% vs. 60% untreated). Preincubation with a metalloproteinase inhibitor prevented L-selectin loss. Conclusions: The mechanism of the differential expression of L-selectin in mouse small intestine and colon appears to be an increased functional activity of a metalloproteinase (sheddase) in the small intestine compared with the colon.

Original languageEnglish (US)
Pages (from-to)965-974
Number of pages10
JournalGastroenterology
Volume114
Issue number5
DOIs
StatePublished - 1998
Externally publishedYes

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L-Selectin
Lymphocytes
Colon
Metalloproteases
Small Intestine
Lymphocyte Homing Receptors
Down-Regulation
Bacterial Antigens
Messenger RNA
Colitis
Reverse Transcription
Flow Cytometry

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Seibold, F., Seibold-Schmid, B., Cong, Y., Feng Yu Shu, Y. S., McCabe, R. P., Weaver, C., & Elson, C. O. (1998). Regional differences in L-selectin expression in murine intestinal lymphocytes. Gastroenterology, 114(5), 965-974. https://doi.org/10.1016/S0016-5085(98)70316-6

Regional differences in L-selectin expression in murine intestinal lymphocytes. / Seibold, F.; Seibold-Schmid, B.; Cong, Yingzi; Feng Yu Shu, Yu Shu; McCabe, R. P.; Weaver, C.; Elson, C. O.

In: Gastroenterology, Vol. 114, No. 5, 1998, p. 965-974.

Research output: Contribution to journalArticle

Seibold, F, Seibold-Schmid, B, Cong, Y, Feng Yu Shu, YS, McCabe, RP, Weaver, C & Elson, CO 1998, 'Regional differences in L-selectin expression in murine intestinal lymphocytes', Gastroenterology, vol. 114, no. 5, pp. 965-974. https://doi.org/10.1016/S0016-5085(98)70316-6
Seibold, F. ; Seibold-Schmid, B. ; Cong, Yingzi ; Feng Yu Shu, Yu Shu ; McCabe, R. P. ; Weaver, C. ; Elson, C. O. / Regional differences in L-selectin expression in murine intestinal lymphocytes. In: Gastroenterology. 1998 ; Vol. 114, No. 5. pp. 965-974.
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AU - McCabe, R. P.

AU - Weaver, C.

AU - Elson, C. O.

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N2 - Background and Aims: The expression of the lymphocyte homing receptor and activation marker L-selectin is different in colon and small intestinal intraepithelial lymphocytes (IELs). In this study, the mechanism of this difference in L-selectin expression was investigated. Methods: L-selectin expression on lymphocytes was measured by flow cytometry. L-selectin messenger RNA (mRNA) was detected by reverse-transcription polymerase chain reaction. L-Selectin expression on peripheral lymphocytes was analyzed after incubation with cytokines, food and bacterial antigens, and homogenates of small and large bowel. Results: L-selectin was expressed by none of the small intestinal IELs but by 30% of those in the colon and by 60% of splenocytes. mRNA for L-selectin was detectable in isolated lymphocytes of all three sites. L-Selectin was downregulated in colon IELs during colitis and up- regulated in small intestinal IELs after in vitro culture for 48 hours. Incubation of splenocytes with small intestinal homogenates led to a rapid down-regulation of L-selectin (1% vs. 60% untreated). Preincubation with a metalloproteinase inhibitor prevented L-selectin loss. Conclusions: The mechanism of the differential expression of L-selectin in mouse small intestine and colon appears to be an increased functional activity of a metalloproteinase (sheddase) in the small intestine compared with the colon.

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