Regional disposal of intravenously infused glucose during prolonged hyperglycemia-hyperinsulinemia

Labros S. Sidossis, Bettina Mittendorfer, Eric Walser, Robert R. Wolfe

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We measured splanchnic and leg glucose uptake during prolonged (i.e., 15 hours), moderate hyperglycemia-hyperinsulinemia (clamp). Plasma free fatty acid (FFA) concentration was maintained at basal concentration during the clamp via infusion of exogenous lipids and heparin in healthy volunteers to create a metabolic profile similar to glucose intolerance (i.e., hyperglycemia-hyperinsulinemia with elevated FFA concentration). During the clamp, glucose was infused at an average rate of 49 ± 4 μmol/kg/min, which resulted in a plasma glucose concentration of 8.8 ± 0.5 mmol/L compared with a concentration of 4.4 ± 0.2 mmol/L in the basal state (P < 0.05). Insulin concentration increased from 5.5 ± 1.1 μU/mL (basal) to 31.3 ± 12.7 μU/mL (clamp; P < 0.05), whereas plasma FFA concentration was similar in the two conditions (3.9 ± 0.5 mmol/L and 4.1 ± 0.5 mmol/L, basal and clamp, respectively). Glucose balance across the splanchnic region switched from net release (-5.8 ± 0.7 μmol/kg/min) in the basal state to net uptake in the clamp (19.8 ± 3.7 μmol/kg/min; P < 0.05) and accounted for approximately 40% of the infused glucose. Glucose uptake across the leg was 0.7 ± 0.2 μmol/kg/min (basal) and 5.5 ± 2.2 μmol/kg/min (clamp; P < 0.05). In summary, tissues in the splanchnic region (i.e., liver) are important for disposal of intravenously infused glucose during prolonged, moderate hyperglycemia-hyperinsulinemia. Accelerated hepatic glucose uptake may disrupt normal liver metabolism, with potentially dangerous consequences for the patient. Measures to control systemic glucose concentration may be necessary to prevent excessive glucose disposal in the liver. Copyright (C) 1999 Elsevier Science Inc.

Original languageEnglish (US)
Pages (from-to)547-554
Number of pages8
JournalJournal of Nutritional Biochemistry
Volume10
Issue number9
DOIs
StatePublished - Sep 1999

Fingerprint

Hyperinsulinism
Hyperglycemia
Clamping devices
Glucose
Viscera
Nonesterified Fatty Acids
Liver
Plasmas
Leg
Glucose Intolerance
Metabolome
Metabolism
Heparin
Healthy Volunteers
Insulin
Lipids
Tissue

Keywords

  • Hepatic vein
  • Insulin resistance
  • Liver
  • Parenteral nutrition
  • Splanchnic balance

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Regional disposal of intravenously infused glucose during prolonged hyperglycemia-hyperinsulinemia. / Sidossis, Labros S.; Mittendorfer, Bettina; Walser, Eric; Wolfe, Robert R.

In: Journal of Nutritional Biochemistry, Vol. 10, No. 9, 09.1999, p. 547-554.

Research output: Contribution to journalArticle

Sidossis, Labros S. ; Mittendorfer, Bettina ; Walser, Eric ; Wolfe, Robert R. / Regional disposal of intravenously infused glucose during prolonged hyperglycemia-hyperinsulinemia. In: Journal of Nutritional Biochemistry. 1999 ; Vol. 10, No. 9. pp. 547-554.
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AB - We measured splanchnic and leg glucose uptake during prolonged (i.e., 15 hours), moderate hyperglycemia-hyperinsulinemia (clamp). Plasma free fatty acid (FFA) concentration was maintained at basal concentration during the clamp via infusion of exogenous lipids and heparin in healthy volunteers to create a metabolic profile similar to glucose intolerance (i.e., hyperglycemia-hyperinsulinemia with elevated FFA concentration). During the clamp, glucose was infused at an average rate of 49 ± 4 μmol/kg/min, which resulted in a plasma glucose concentration of 8.8 ± 0.5 mmol/L compared with a concentration of 4.4 ± 0.2 mmol/L in the basal state (P < 0.05). Insulin concentration increased from 5.5 ± 1.1 μU/mL (basal) to 31.3 ± 12.7 μU/mL (clamp; P < 0.05), whereas plasma FFA concentration was similar in the two conditions (3.9 ± 0.5 mmol/L and 4.1 ± 0.5 mmol/L, basal and clamp, respectively). Glucose balance across the splanchnic region switched from net release (-5.8 ± 0.7 μmol/kg/min) in the basal state to net uptake in the clamp (19.8 ± 3.7 μmol/kg/min; P < 0.05) and accounted for approximately 40% of the infused glucose. Glucose uptake across the leg was 0.7 ± 0.2 μmol/kg/min (basal) and 5.5 ± 2.2 μmol/kg/min (clamp; P < 0.05). In summary, tissues in the splanchnic region (i.e., liver) are important for disposal of intravenously infused glucose during prolonged, moderate hyperglycemia-hyperinsulinemia. Accelerated hepatic glucose uptake may disrupt normal liver metabolism, with potentially dangerous consequences for the patient. Measures to control systemic glucose concentration may be necessary to prevent excessive glucose disposal in the liver. Copyright (C) 1999 Elsevier Science Inc.

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