Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein

Yutaka Ito, Kazuhiko Yamasaki, Junji Iwahara, Tohru Terada, Akihide Kamiya, Mikako Shirouzu, Yutaka Muto, Gota Kawai, Shigeyuki Yokoyama, Ernest D. Laue, Markus Wälchli, Takehiko Shibata, Susumu Nishimura, Tatsuo Miyazawa

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

The backbone 1H, 13C, and 15N resonances of the c-Ha-Ras protein [a truncated version consisting of residues 1-171, Ras(1-171)] bound with GMPPNP (a slowly hydrolyzable analogue of GTP) were assigned and compared with those of the GDP-bound Ras(1-171). The backbone amide resonances of amino acid residues 10-13, 21, 31-39, 57-64, and 71 of Ras(1-171)·GMPPNP, but not those of Ras(1- 71)·GDP, were extremely broadened, whereas other residues of Ras(1-171)·GMPPNP exhibited amide resonances nearly as sharp as those of Ras(1-171)·GDP. The residues exhibiting the extreme broadening, except for residues 21 and 71, are localized in three functional loop regions [loops L1, L2 (switch), and L4 (switch II)], which are involved in hydrolysis of GTP and interactions with other proteins. From the temperature and magnetic field strength dependencies of the backbone amide resonance intensities, the extreme broadening was ascribed to the exchange at an intermediate rate on the NMR time scale. It was shown that the Ras(1-171) protein bound with GTP or GTPγS (another slowly hydrolyzable analogue of GTP) exhibits the same type of broadening. Therefore, it is a characteristic feature of the GTP- bound form of Ras that the L1, L2, and L4 loop regions, but not other regions, are in a rather slow interconversion between two or more stable conformers. This phenomenon, termed a 'regional polysterism', of these loop regions may be related with their multifunctionality: the GTP-dependent interactions with several downstream target groups such as the Raf and RalGDS families and also with the GTPase activating protein (GAP) family. In fact, the binding of Ras(1-171)·GMPPNP with the Ras-binding domain (residues 51- 131) of c-Raf-1 was shown to eliminate the regional polysterism nearly completely. It was indicated, therefore, that each target/regulator selects its appropriate conformer among those presented by the 'polysteric' binding interface of Ras. As the downstream target groups exhibit no apparent sequence homology to each other, it is possible that one target group prefers a conformer different from that preferred by another group. The involvement of loop L1 in the regional polysterism might suggest that the negative regulators, GAPs, bind to the polysteric binding interface (loops L2 and L4) of Ras and cooperatively select a conformer suitable for transition of the GTPase catalytic center, involving loops L1 and L4, into the highly active state.

Original languageEnglish (US)
Pages (from-to)9109-9119
Number of pages11
JournalBiochemistry
Volume36
Issue number30
DOIs
StatePublished - Jul 29 1997
Externally publishedYes

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Proto-Oncogene Proteins p21(ras)
ras Proteins
Guanosine Triphosphate
Amides
Switches
GTPase-Activating Proteins
GTP Phosphohydrolases
Magnetic Fields
Sequence Homology
Hydrolysis
Proteins
Temperature distribution
Nuclear magnetic resonance
Magnetic fields
Amino Acids
Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Ito, Y., Yamasaki, K., Iwahara, J., Terada, T., Kamiya, A., Shirouzu, M., ... Miyazawa, T. (1997). Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein. Biochemistry, 36(30), 9109-9119. https://doi.org/10.1021/bi970296u

Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein. / Ito, Yutaka; Yamasaki, Kazuhiko; Iwahara, Junji; Terada, Tohru; Kamiya, Akihide; Shirouzu, Mikako; Muto, Yutaka; Kawai, Gota; Yokoyama, Shigeyuki; Laue, Ernest D.; Wälchli, Markus; Shibata, Takehiko; Nishimura, Susumu; Miyazawa, Tatsuo.

In: Biochemistry, Vol. 36, No. 30, 29.07.1997, p. 9109-9119.

Research output: Contribution to journalArticle

Ito, Y, Yamasaki, K, Iwahara, J, Terada, T, Kamiya, A, Shirouzu, M, Muto, Y, Kawai, G, Yokoyama, S, Laue, ED, Wälchli, M, Shibata, T, Nishimura, S & Miyazawa, T 1997, 'Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein', Biochemistry, vol. 36, no. 30, pp. 9109-9119. https://doi.org/10.1021/bi970296u
Ito Y, Yamasaki K, Iwahara J, Terada T, Kamiya A, Shirouzu M et al. Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein. Biochemistry. 1997 Jul 29;36(30):9109-9119. https://doi.org/10.1021/bi970296u
Ito, Yutaka ; Yamasaki, Kazuhiko ; Iwahara, Junji ; Terada, Tohru ; Kamiya, Akihide ; Shirouzu, Mikako ; Muto, Yutaka ; Kawai, Gota ; Yokoyama, Shigeyuki ; Laue, Ernest D. ; Wälchli, Markus ; Shibata, Takehiko ; Nishimura, Susumu ; Miyazawa, Tatsuo. / Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein. In: Biochemistry. 1997 ; Vol. 36, No. 30. pp. 9109-9119.
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T1 - Regional polysterism in the GTP-bound form of the human c-Ha-Ras protein

AU - Ito, Yutaka

AU - Yamasaki, Kazuhiko

AU - Iwahara, Junji

AU - Terada, Tohru

AU - Kamiya, Akihide

AU - Shirouzu, Mikako

AU - Muto, Yutaka

AU - Kawai, Gota

AU - Yokoyama, Shigeyuki

AU - Laue, Ernest D.

AU - Wälchli, Markus

AU - Shibata, Takehiko

AU - Nishimura, Susumu

AU - Miyazawa, Tatsuo

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N2 - The backbone 1H, 13C, and 15N resonances of the c-Ha-Ras protein [a truncated version consisting of residues 1-171, Ras(1-171)] bound with GMPPNP (a slowly hydrolyzable analogue of GTP) were assigned and compared with those of the GDP-bound Ras(1-171). The backbone amide resonances of amino acid residues 10-13, 21, 31-39, 57-64, and 71 of Ras(1-171)·GMPPNP, but not those of Ras(1- 71)·GDP, were extremely broadened, whereas other residues of Ras(1-171)·GMPPNP exhibited amide resonances nearly as sharp as those of Ras(1-171)·GDP. The residues exhibiting the extreme broadening, except for residues 21 and 71, are localized in three functional loop regions [loops L1, L2 (switch), and L4 (switch II)], which are involved in hydrolysis of GTP and interactions with other proteins. From the temperature and magnetic field strength dependencies of the backbone amide resonance intensities, the extreme broadening was ascribed to the exchange at an intermediate rate on the NMR time scale. It was shown that the Ras(1-171) protein bound with GTP or GTPγS (another slowly hydrolyzable analogue of GTP) exhibits the same type of broadening. Therefore, it is a characteristic feature of the GTP- bound form of Ras that the L1, L2, and L4 loop regions, but not other regions, are in a rather slow interconversion between two or more stable conformers. This phenomenon, termed a 'regional polysterism', of these loop regions may be related with their multifunctionality: the GTP-dependent interactions with several downstream target groups such as the Raf and RalGDS families and also with the GTPase activating protein (GAP) family. In fact, the binding of Ras(1-171)·GMPPNP with the Ras-binding domain (residues 51- 131) of c-Raf-1 was shown to eliminate the regional polysterism nearly completely. It was indicated, therefore, that each target/regulator selects its appropriate conformer among those presented by the 'polysteric' binding interface of Ras. As the downstream target groups exhibit no apparent sequence homology to each other, it is possible that one target group prefers a conformer different from that preferred by another group. The involvement of loop L1 in the regional polysterism might suggest that the negative regulators, GAPs, bind to the polysteric binding interface (loops L2 and L4) of Ras and cooperatively select a conformer suitable for transition of the GTPase catalytic center, involving loops L1 and L4, into the highly active state.

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