Regional substrate metabolism during prolonged hyperglycemia in humans

L. S. Sidossis, B. Mittendorfer, Eric Walser, D. L. Chinkes, R. R. Wolfe

Research output: Contribution to journalArticle

Abstract

The effect of prolonged (15 hours) hyperglycemia (glucose ∼160 mg/dl) on splanchnic and leg substrate metabolism was investigated. Catheters were inserted in a peripheral vein for tracer infusion and in a femoral artery, femoral vein and a hepatic vein for blood sampling. [U-13C] fatty acids (0.03 μmol/kg.min) were infused for 15h. Plasma FFA enrichment and concentration were maintained constant by infusion of lipids and heparin. Splanchnic and leg blood flow was measured by constant infusion of Indocyanin Green. Results: Neither splanchnic nor leg blood flow changed significantly during the clamp. Splanchnic Bed: Net glucose balance switched from net release during basal (9±1 mg/dl) to net uptake during the clamp (36±12 mg/dl, p<0.05). Fatty acid uptake by the splanchnic bed did not change with hyperglycemia. However, fatty acid oxidation decreased by 57.7% (p<0.05) during the clamp. Leg: Glucose uptake by the leg increased from 1.4±0.8 mg/dl during basal to 24±13 mg/dl during the clamp (p<0.05). Fatty acid uptake decreased from 0.62±0.18 to 0.45±0.16 μmol/kg.min during the clamp (p<0.05), whereas fatty acid oxidation dropped by 63% (p<0.05)). Conclusion: Prolonged hyperglycemia increases glucose but attenuates fatty acid oxidation across both the splanchnic region and the leg in human volunteers. The decrease in splanchnic fatty acid oxidation during the clamp was not accompanied by a drop in FFA uptake, suggesting channeling of FFA into triacylglycerols. This could explain the increased triacylglycerol concentration observed in high carbohydrate feeding.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997

Fingerprint

Viscera
hyperglycemia
Metabolism
Hyperglycemia
Clamping devices
legs
beta oxidation
Fatty Acids
Leg
metabolism
uptake mechanisms
Substrates
glucose
Glucose
Oxidation
thighs
Blood
fatty acids
blood flow
bed nets

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Sidossis, L. S., Mittendorfer, B., Walser, E., Chinkes, D. L., & Wolfe, R. R. (1997). Regional substrate metabolism during prolonged hyperglycemia in humans. FASEB Journal, 11(3).

Regional substrate metabolism during prolonged hyperglycemia in humans. / Sidossis, L. S.; Mittendorfer, B.; Walser, Eric; Chinkes, D. L.; Wolfe, R. R.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Sidossis, LS, Mittendorfer, B, Walser, E, Chinkes, DL & Wolfe, RR 1997, 'Regional substrate metabolism during prolonged hyperglycemia in humans', FASEB Journal, vol. 11, no. 3.
Sidossis LS, Mittendorfer B, Walser E, Chinkes DL, Wolfe RR. Regional substrate metabolism during prolonged hyperglycemia in humans. FASEB Journal. 1997;11(3).
Sidossis, L. S. ; Mittendorfer, B. ; Walser, Eric ; Chinkes, D. L. ; Wolfe, R. R. / Regional substrate metabolism during prolonged hyperglycemia in humans. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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N2 - The effect of prolonged (15 hours) hyperglycemia (glucose ∼160 mg/dl) on splanchnic and leg substrate metabolism was investigated. Catheters were inserted in a peripheral vein for tracer infusion and in a femoral artery, femoral vein and a hepatic vein for blood sampling. [U-13C] fatty acids (0.03 μmol/kg.min) were infused for 15h. Plasma FFA enrichment and concentration were maintained constant by infusion of lipids and heparin. Splanchnic and leg blood flow was measured by constant infusion of Indocyanin Green. Results: Neither splanchnic nor leg blood flow changed significantly during the clamp. Splanchnic Bed: Net glucose balance switched from net release during basal (9±1 mg/dl) to net uptake during the clamp (36±12 mg/dl, p<0.05). Fatty acid uptake by the splanchnic bed did not change with hyperglycemia. However, fatty acid oxidation decreased by 57.7% (p<0.05) during the clamp. Leg: Glucose uptake by the leg increased from 1.4±0.8 mg/dl during basal to 24±13 mg/dl during the clamp (p<0.05). Fatty acid uptake decreased from 0.62±0.18 to 0.45±0.16 μmol/kg.min during the clamp (p<0.05), whereas fatty acid oxidation dropped by 63% (p<0.05)). Conclusion: Prolonged hyperglycemia increases glucose but attenuates fatty acid oxidation across both the splanchnic region and the leg in human volunteers. The decrease in splanchnic fatty acid oxidation during the clamp was not accompanied by a drop in FFA uptake, suggesting channeling of FFA into triacylglycerols. This could explain the increased triacylglycerol concentration observed in high carbohydrate feeding.

AB - The effect of prolonged (15 hours) hyperglycemia (glucose ∼160 mg/dl) on splanchnic and leg substrate metabolism was investigated. Catheters were inserted in a peripheral vein for tracer infusion and in a femoral artery, femoral vein and a hepatic vein for blood sampling. [U-13C] fatty acids (0.03 μmol/kg.min) were infused for 15h. Plasma FFA enrichment and concentration were maintained constant by infusion of lipids and heparin. Splanchnic and leg blood flow was measured by constant infusion of Indocyanin Green. Results: Neither splanchnic nor leg blood flow changed significantly during the clamp. Splanchnic Bed: Net glucose balance switched from net release during basal (9±1 mg/dl) to net uptake during the clamp (36±12 mg/dl, p<0.05). Fatty acid uptake by the splanchnic bed did not change with hyperglycemia. However, fatty acid oxidation decreased by 57.7% (p<0.05) during the clamp. Leg: Glucose uptake by the leg increased from 1.4±0.8 mg/dl during basal to 24±13 mg/dl during the clamp (p<0.05). Fatty acid uptake decreased from 0.62±0.18 to 0.45±0.16 μmol/kg.min during the clamp (p<0.05), whereas fatty acid oxidation dropped by 63% (p<0.05)). Conclusion: Prolonged hyperglycemia increases glucose but attenuates fatty acid oxidation across both the splanchnic region and the leg in human volunteers. The decrease in splanchnic fatty acid oxidation during the clamp was not accompanied by a drop in FFA uptake, suggesting channeling of FFA into triacylglycerols. This could explain the increased triacylglycerol concentration observed in high carbohydrate feeding.

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