Regulated expression of the Saccharomyces cerevisiae DNA repair gene RAD7 in response to DNA damage and during sporulation

Jeffery S. Jones, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The RAD7 gene of Saccharomyces cerevisiae affects the proficiency of excision repair of DNA damaged by UV light. Here, we report our studies on the regulation of the RAD7 gene in response to UV irradiation and during sporulation. RAD7 transcript levels increased 6-fold within 40 min of exposure of cells to 37 J/m2 of UV light. Higher UV doses also elicited rapid increases in the level of PAD7 mRNA. RAD7 mRNA levels increased in sporulating MATa/MATα diploid cells, but not in the asporogenous MATa/MATa strain exposed to sporulation conditions. The increase in RAD7 mRNA level in MATa/MATα cells was 15-fold after 6 h and 9-fold after 7 h in sporulation medium; thereafter, RAD7 mRNA levels declined. Periodic transcription of RAD7 during sporulation suggests a role for RAD7 in this process.

Original languageEnglish (US)
Pages (from-to)3281-3285
Number of pages5
JournalNucleic Acids Research
Volume18
Issue number11
StatePublished - Jun 11 1990
Externally publishedYes

Fingerprint

Saccharomyces Cerevisiae
DNA Repair
Messenger RNA
Yeast
DNA Damage
Repair
Saccharomyces cerevisiae
DNA
Damage
Genes
Gene
Fold
Ultraviolet Rays
Ultraviolet radiation
Cell
Transcription
Diploidy
Irradiation
Dose

ASJC Scopus subject areas

  • Genetics
  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)

Cite this

Regulated expression of the Saccharomyces cerevisiae DNA repair gene RAD7 in response to DNA damage and during sporulation. / Jones, Jeffery S.; Prakash, Louise; Prakash, Satya.

In: Nucleic Acids Research, Vol. 18, No. 11, 11.06.1990, p. 3281-3285.

Research output: Contribution to journalArticle

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