Regulation of γ-glutamylcysteine synthetase activity by nerve growth factor

Zhaohui Pan, Regino Perez-Polo

    Research output: Contribution to journalArticle

    12 Citations (Scopus)

    Abstract

    Nerve growth factor is a neurotrophic factor that regulates neuronal cell development, maintenance, and injury responses in the peripheral and central nervous system. Nerve growth factor reduces injury owing to oxidative stress in rat pheochromocytoma (PC12) cells by increasing intracellular glutathione, in part owing to its stimulation of the activity of γ-glutamylcysteine synthetase, which is the rate-limiting enzyme in the synthesis of glutathione. Here we show that nerve growth factor did not increase the activity of γ-glutamylcysteine synthetase in PC12 cells at the transcriptional level. Rather, nerve growth factor enhanced the stability of γ-glutamylcysteine synthetase mRNA in PC12 cells. These results suggest that, during oxidative stress, nerve growth factor extended the half-life of γ-glutamylcysteine synthetase mRNA, thus increasing γ-glutamylcysteine synthetase mRNA levels compared to nerve growth factor-deprived PC12 cells.

    Original languageEnglish (US)
    Pages (from-to)559-566
    Number of pages8
    JournalInternational Journal of Developmental Neuroscience
    Volume14
    Issue number5
    DOIs
    StatePublished - Aug 1996

    Fingerprint

    Glutamate-Cysteine Ligase
    Nerve Growth Factor
    PC12 Cells
    Messenger RNA
    Glutathione
    Oxidative Stress
    Peripheral Nervous System
    Nerve Growth Factors
    Wounds and Injuries
    Pheochromocytoma
    Half-Life
    Central Nervous System
    Maintenance
    Enzymes

    Keywords

    • γ-glutamylcysteine synthetase
    • Glutathione
    • Nerve growth factor
    • PC12

    ASJC Scopus subject areas

    • Developmental Biology
    • Developmental Neuroscience

    Cite this

    Regulation of γ-glutamylcysteine synthetase activity by nerve growth factor. / Pan, Zhaohui; Perez-Polo, Regino.

    In: International Journal of Developmental Neuroscience, Vol. 14, No. 5, 08.1996, p. 559-566.

    Research output: Contribution to journalArticle

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