Regulation of 4-hydroxynonenal mediated signaling by glutathione S-transferases

Yogesh C. Awasthi, Ghulam Ansari, Sanjay Awasthi

Research output: Contribution to journalArticle

89 Citations (Scopus)

Abstract

4-Hydroxy-trans-2-nonenal (HNE) was initially considered to be merely a toxic end product of lipid peroxidation that contributed to oxidative stress-related pathogenesis. However, in recent years its physiological role as an important "signaling molecule" has been established. HNE can modulate various signaling pathways in a concentration-dependent manner. Glutathione S-transferases (GSTs) are major determinants of the intracellular concentration of HNE, because these enzymes account for the metabolism of most cellular HNE through its conjugation to glutathione. Evidence is emerging that GSTs are involved in the regulation of the HNE-mediated signaling processes. Against the backdrop of our current understanding on the formation, metabolism, and role of HNE in signaling processes, the physiological role of GSTs in regulation of HNE-mediated signaling processes is critically evaluated in this chapter. Available evidence strongly suggests that besides their well-established pharmacological role of detoxifying xenobiotics, GSTs also play an important physiological role in the regulation of cellular signaling processes.

Original languageEnglish (US)
Article number24
Pages (from-to)379-407
Number of pages29
JournalMethods in Enzymology
Volume401
DOIs
StatePublished - 2005

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Glutathione Transferase
Metabolism
Physiological Phenomena
Cell signaling
Oxidative stress
Poisons
Xenobiotics
2-nonenal
4-hydroxy-2-nonenal
Lipid Peroxidation
Glutathione
Oxidative Stress
Pharmacology
Lipids
Molecules
Enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of 4-hydroxynonenal mediated signaling by glutathione S-transferases. / Awasthi, Yogesh C.; Ansari, Ghulam; Awasthi, Sanjay.

In: Methods in Enzymology, Vol. 401, 24, 2005, p. 379-407.

Research output: Contribution to journalArticle

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