Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence

Hiroshi Saito, John Papaconstantinou, Hiroyuki Sato, Samuel Goldstein

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

We report here a novel cDNA clone with a predicted protein sequence similar to lysyl oxidase. This full-length cDNA clone of 3432 base pairs (WS9-14) was isolated from human fibroblasts on the basis of its overexpression in senescent cells. It encodes an 87-kDa polypeptide, whose protein is a member of the scavenger receptor cysteine-rich family, because it contains four scavenger receptor cysteine-rich domains that are found in several secreted or cell surface proteins. The WS9-14 protein has a 48% identity with both lysyl oxidase and lysyl oxidase-like protein at a region corresponding to exons 2-6, implying the existence of a lysyl oxidase gene family. The pattern of WS9-14 gene expression by fibroblasts parallels pro- collagen I-α1 expression. Its mRNA level is induced by transforming growth factor β-1 and indomethacin and inhibited by phorbol ester and retinoic acid. WS9-14 is abundantly expressed in all tumor cell lines examined that attach to culture dishes but not in cell lines that grow in suspension and is also up-regulated in senescent fibroblasts. These results suggest that WS9- 14 gene encodes an extracellular protein that may be specifically involved in cell adhesion and senescence.

Original languageEnglish (US)
Pages (from-to)8157-8160
Number of pages4
JournalJournal of Biological Chemistry
Volume272
Issue number13
DOIs
StatePublished - Mar 28 1997

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Protein-Lysine 6-Oxidase
Gene encoding
Cell Aging
Adhesion
Fibroblasts
Scavenger Receptors
Genes
Proteins
Cysteine
Complementary DNA
Clone Cells
Cells
Cell adhesion
Transforming Growth Factors
Phorbol Esters
Tretinoin
Tumor Cell Line
Cell culture
Gene expression
Cell Adhesion

ASJC Scopus subject areas

  • Biochemistry

Cite this

Regulation of a novel gene encoding a lysyl oxidase-related protein in cellular adhesion and senescence. / Saito, Hiroshi; Papaconstantinou, John; Sato, Hiroyuki; Goldstein, Samuel.

In: Journal of Biological Chemistry, Vol. 272, No. 13, 28.03.1997, p. 8157-8160.

Research output: Contribution to journalArticle

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