Regulation of IL-1β-induced GM-CSF production in human airway smooth muscle cells by carbon monoxide

Ruiping Song, Wen Ning, Fang Liu, Bill Ameredes, William Calhoun, Leo E. Otterbein, Augustine M K Choi

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Asthma, a chronic inflammatory disease of the airways, involves the increased expression of inflammatory mediators, including granulocyte-monocyte colony-stimulating factor (GM-CSF). Heme oxygenase-1 (HO-1), a stress-response protein, confers protection against oxidative stress. We hypothesized that carbon monoxide (CO), a byproduct of HO-1-dependent heme catabolism, regulates GM-CSF synthesis in human airway smooth muscle cells (HASMC). IL-1β treatment induced a time-dependent induction of GM-CSF in HASMC. Furthermore, IL-1β stimulated the major MAPK pathways, including ERK1/ERK2, JNK, and p38 MAPK. Exposure of HASMC to CO at low concentration (250 ppm) markedly inhibited IL-1β-induced GM-CSF synthesis (>90%) compared with air-treated controls. CO treatment inhibited IL-1β-induced ERK1/2 activation but did not inhibit JNK and p38 MAPK. Furthermore, CO increased cGMP levels in HASMC. Inhibition of guanylate cyclase by IH-[1,2,4] oxadiazolo[4,3-alquinoxalin-1-1 (ODQ) abolished the inhibitory effects of CO on GM-CSF synthesis and ERK1/2 activation. Collectively, these data demonstrate that the inhibitory effect of CO on GM-CSF synthesis depends on ERK1/2 MAPK and guanylate cyclase/cGMP-dependent pathways.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume284
Issue number1 28-1
StatePublished - Jan 1 2003
Externally publishedYes

Fingerprint

Granulocyte Colony-Stimulating Factor
Carbon Monoxide
Interleukin-1
Smooth Muscle Myocytes
Monocytes
Heme Oxygenase-1
p38 Mitogen-Activated Protein Kinases
MAP Kinase Signaling System
Guanylate Cyclase
Heat-Shock Proteins
Heme
Oxidative Stress
Chronic Disease
Asthma
Air

Keywords

  • Cyclic guanosine monophosphate
  • Granulocyte-monocyte colony-stimulating factor
  • Mitogen activated protein kinase interleukin-1β

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine
  • Cell Biology
  • Physiology

Cite this

Regulation of IL-1β-induced GM-CSF production in human airway smooth muscle cells by carbon monoxide. / Song, Ruiping; Ning, Wen; Liu, Fang; Ameredes, Bill; Calhoun, William; Otterbein, Leo E.; Choi, Augustine M K.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 284, No. 1 28-1, 01.01.2003.

Research output: Contribution to journalArticle

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