Regulation of plasma fatty acid oxidation during low- and high-intensity exercise

Labros S. Sidossis, Amalia Gastaldelli, Samuel Klein, Robert R. Wolfe

Research output: Contribution to journalArticle

119 Citations (Scopus)

Abstract

In the present study we examined the hypothesis that fatty acid oxidation is less during high-intensity exercise than during moderate- intensity exercise because of inhibition of longchain fatty acid entry into the mitochondria. Six volunteers exercised at 40% peak oxygen consumption (V̇O2(peak)) for 60 min and at 80% V̇O2(peak) for 30 min on two different occasions. [1-13C]oleate, a long-chain fatty acid, and [1-14C]octanoate, a medium-chain fatty acid, were infused for the duration of the studies. Lipids and heparin were infused during exercise at 80% V̇O2(peak) to prevent the expected decrease in plasma free fatty acid (FFA) concentration. Plasma oleate and total FFA availability were similar in the two experiments. Oleate oxidation decreased from 2.8 ± 0.6 (40% V̇O2(peak)) to 1.8 ± 0.2 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05), whereas octanoate oxidation increased from 1.0e-05 ± 1.0e-06 (40% V̇O2(peak)) to 1.3e-05 + 5.1e-06 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05). Furthermore, the percentage of oleate uptake oxidized decreased from 67.7 ± 2.8% (40% V̇O2(peak)) to 51.8 ± 4.6% (80% V̇O2(peak), P < 0.05), whereas the percentage of octanoate oxidized was similar during exercise at 40 and 80% V̇O2(peak) (84.8 ± 2.7 vs. 89.3 ± 2.7%, respectively). Our data suggest that, in addition to suboptimal FFA availability, fatty acid oxidation is likely limited during high-intensity exercise because of direct inhibition of long-chain fatty acid entry into mitochondria.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume272
Issue number6 35-6
StatePublished - Jun 1997

Fingerprint

Fatty Acids
Exercise
Oleic Acid
Plasmas
Oxidation
Nonesterified Fatty Acids
Mitochondria
Availability
Oxygen Consumption
Heparin
Volunteers
Oxygen
Lipids
octanoic acid
Experiments

Keywords

  • Carnitine palmitoyltransferase
  • Malonyl-coenzyme A
  • Medium- chain fatty acids
  • Mitochondria

ASJC Scopus subject areas

  • Physiology
  • Endocrinology
  • Biochemistry
  • Physiology (medical)

Cite this

Regulation of plasma fatty acid oxidation during low- and high-intensity exercise. / Sidossis, Labros S.; Gastaldelli, Amalia; Klein, Samuel; Wolfe, Robert R.

In: American Journal of Physiology - Endocrinology and Metabolism, Vol. 272, No. 6 35-6, 06.1997.

Research output: Contribution to journalArticle

Sidossis, Labros S. ; Gastaldelli, Amalia ; Klein, Samuel ; Wolfe, Robert R. / Regulation of plasma fatty acid oxidation during low- and high-intensity exercise. In: American Journal of Physiology - Endocrinology and Metabolism. 1997 ; Vol. 272, No. 6 35-6.
@article{d5a1124be7fd4416ad9f3b6c1de4a355,
title = "Regulation of plasma fatty acid oxidation during low- and high-intensity exercise",
abstract = "In the present study we examined the hypothesis that fatty acid oxidation is less during high-intensity exercise than during moderate- intensity exercise because of inhibition of longchain fatty acid entry into the mitochondria. Six volunteers exercised at 40{\%} peak oxygen consumption (V̇O2(peak)) for 60 min and at 80{\%} V̇O2(peak) for 30 min on two different occasions. [1-13C]oleate, a long-chain fatty acid, and [1-14C]octanoate, a medium-chain fatty acid, were infused for the duration of the studies. Lipids and heparin were infused during exercise at 80{\%} V̇O2(peak) to prevent the expected decrease in plasma free fatty acid (FFA) concentration. Plasma oleate and total FFA availability were similar in the two experiments. Oleate oxidation decreased from 2.8 ± 0.6 (40{\%} V̇O2(peak)) to 1.8 ± 0.2 μmol · kg-1 · min-1 (80{\%} V̇O2(peak), P < 0.05), whereas octanoate oxidation increased from 1.0e-05 ± 1.0e-06 (40{\%} V̇O2(peak)) to 1.3e-05 + 5.1e-06 μmol · kg-1 · min-1 (80{\%} V̇O2(peak), P < 0.05). Furthermore, the percentage of oleate uptake oxidized decreased from 67.7 ± 2.8{\%} (40{\%} V̇O2(peak)) to 51.8 ± 4.6{\%} (80{\%} V̇O2(peak), P < 0.05), whereas the percentage of octanoate oxidized was similar during exercise at 40 and 80{\%} V̇O2(peak) (84.8 ± 2.7 vs. 89.3 ± 2.7{\%}, respectively). Our data suggest that, in addition to suboptimal FFA availability, fatty acid oxidation is likely limited during high-intensity exercise because of direct inhibition of long-chain fatty acid entry into mitochondria.",
keywords = "Carnitine palmitoyltransferase, Malonyl-coenzyme A, Medium- chain fatty acids, Mitochondria",
author = "Sidossis, {Labros S.} and Amalia Gastaldelli and Samuel Klein and Wolfe, {Robert R.}",
year = "1997",
month = "6",
language = "English (US)",
volume = "272",
journal = "American Journal of Physiology - Endocrinology and Metabolism",
issn = "0193-1849",
publisher = "American Physiological Society",
number = "6 35-6",

}

TY - JOUR

T1 - Regulation of plasma fatty acid oxidation during low- and high-intensity exercise

AU - Sidossis, Labros S.

AU - Gastaldelli, Amalia

AU - Klein, Samuel

AU - Wolfe, Robert R.

PY - 1997/6

Y1 - 1997/6

N2 - In the present study we examined the hypothesis that fatty acid oxidation is less during high-intensity exercise than during moderate- intensity exercise because of inhibition of longchain fatty acid entry into the mitochondria. Six volunteers exercised at 40% peak oxygen consumption (V̇O2(peak)) for 60 min and at 80% V̇O2(peak) for 30 min on two different occasions. [1-13C]oleate, a long-chain fatty acid, and [1-14C]octanoate, a medium-chain fatty acid, were infused for the duration of the studies. Lipids and heparin were infused during exercise at 80% V̇O2(peak) to prevent the expected decrease in plasma free fatty acid (FFA) concentration. Plasma oleate and total FFA availability were similar in the two experiments. Oleate oxidation decreased from 2.8 ± 0.6 (40% V̇O2(peak)) to 1.8 ± 0.2 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05), whereas octanoate oxidation increased from 1.0e-05 ± 1.0e-06 (40% V̇O2(peak)) to 1.3e-05 + 5.1e-06 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05). Furthermore, the percentage of oleate uptake oxidized decreased from 67.7 ± 2.8% (40% V̇O2(peak)) to 51.8 ± 4.6% (80% V̇O2(peak), P < 0.05), whereas the percentage of octanoate oxidized was similar during exercise at 40 and 80% V̇O2(peak) (84.8 ± 2.7 vs. 89.3 ± 2.7%, respectively). Our data suggest that, in addition to suboptimal FFA availability, fatty acid oxidation is likely limited during high-intensity exercise because of direct inhibition of long-chain fatty acid entry into mitochondria.

AB - In the present study we examined the hypothesis that fatty acid oxidation is less during high-intensity exercise than during moderate- intensity exercise because of inhibition of longchain fatty acid entry into the mitochondria. Six volunteers exercised at 40% peak oxygen consumption (V̇O2(peak)) for 60 min and at 80% V̇O2(peak) for 30 min on two different occasions. [1-13C]oleate, a long-chain fatty acid, and [1-14C]octanoate, a medium-chain fatty acid, were infused for the duration of the studies. Lipids and heparin were infused during exercise at 80% V̇O2(peak) to prevent the expected decrease in plasma free fatty acid (FFA) concentration. Plasma oleate and total FFA availability were similar in the two experiments. Oleate oxidation decreased from 2.8 ± 0.6 (40% V̇O2(peak)) to 1.8 ± 0.2 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05), whereas octanoate oxidation increased from 1.0e-05 ± 1.0e-06 (40% V̇O2(peak)) to 1.3e-05 + 5.1e-06 μmol · kg-1 · min-1 (80% V̇O2(peak), P < 0.05). Furthermore, the percentage of oleate uptake oxidized decreased from 67.7 ± 2.8% (40% V̇O2(peak)) to 51.8 ± 4.6% (80% V̇O2(peak), P < 0.05), whereas the percentage of octanoate oxidized was similar during exercise at 40 and 80% V̇O2(peak) (84.8 ± 2.7 vs. 89.3 ± 2.7%, respectively). Our data suggest that, in addition to suboptimal FFA availability, fatty acid oxidation is likely limited during high-intensity exercise because of direct inhibition of long-chain fatty acid entry into mitochondria.

KW - Carnitine palmitoyltransferase

KW - Malonyl-coenzyme A

KW - Medium- chain fatty acids

KW - Mitochondria

UR - http://www.scopus.com/inward/record.url?scp=0030741642&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030741642&partnerID=8YFLogxK

M3 - Article

C2 - 9227453

AN - SCOPUS:0030741642

VL - 272

JO - American Journal of Physiology - Endocrinology and Metabolism

JF - American Journal of Physiology - Endocrinology and Metabolism

SN - 0193-1849

IS - 6 35-6

ER -