Regulation of progesterone receptor-mediated transcription by phosphorylation

Larry Denner, Nancy L. Weigel, Beth L. Maxwell, William T. Schrader, Bert W. O'Malley

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299 Scopus citations

Abstract

The progesterone receptor (PR) in the chicken oviduct is a phosphoprotein that regulates gene transcription in the presence of progesterone. Treatment with progesterone in vivo stimulates phosphorylation of the progesterone receptor. With transient transfection assays, the present work has tested whether phosphorylation participates in the regulation of PR-mediated transcription. Treatment with 8-bromo-cyclic adenosine monophosphate (8-Br cAMP), a stimulator of cAMP-depcndent protein kinase [protein kinase A (PKA)], mimicked progesterone-dependent, receptor-mediated transcription in the absence of progesterone. Inhibition of PKA blocked hormone action. Treatment with okadaic acid, an inhibitor of protein phosphatascs 1 and 2A, stimulated transcription in a manner similar to that of progesterone. These observations suggest that phosphorylation of the PR or other proteins in the transcription complex can modulate PR-mediated transcription in vivo.

Original languageEnglish (US)
Pages (from-to)1740-1743
Number of pages4
JournalScience
Volume250
Issue number4988
StatePublished - Dec 21 1990
Externally publishedYes

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Cite this

Denner, L., Weigel, N. L., Maxwell, B. L., Schrader, W. T., & O'Malley, B. W. (1990). Regulation of progesterone receptor-mediated transcription by phosphorylation. Science, 250(4988), 1740-1743.