Regulation of progesterone receptor-mediated transcription by phosphorylation

Larry A. Denner, Nancy L. Weigel, Beth L. Maxwell, William T. Schrader, Bert W. O'Malley

Research output: Contribution to journalArticle

303 Scopus citations

Abstract

The progesterone receptor (PR) in the chicken oviduct is a phosphoprotein that regulates gene transcription in the presence of progesterone. Treatment with progesterone in vivo stimulates phosphorylation of the progesterone receptor. With transient transaction assays, the present work has tested whether phosphorylation participates in the regulation of PR-mediated transcription. Treatment with 8-bromo-cyclic adenosine monophosphate (8-Br cAMP), a stimulator of cAMP-dependent protein kinase [protein kinase A (PKA)], mimicked progesterone-dependent, receptor-mediated transcription in the absence of progesterone. Inhibition of PKA blocked hormone action. Treatment with okadaic acid, an inhibitor of protein phosphatases 1 and 2A, stimulated transcription in a manner similar to that of progesterone. These observations suggest that phosphorylation of the PR or other proteins in the transcription complex can modulate PR-mediated transcription in vivo.

Original languageEnglish (US)
Pages (from-to)1740-1743
Number of pages4
JournalScience
Volume250
Issue number4988
DOIs
StatePublished - Jan 1 1990
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Denner, L. A., Weigel, N. L., Maxwell, B. L., Schrader, W. T., & O'Malley, B. W. (1990). Regulation of progesterone receptor-mediated transcription by phosphorylation. Science, 250(4988), 1740-1743. https://doi.org/10.1126/science.2176746