Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy

Christopher Fry, Jonah D. Lee, Janna R. Jackson, Tyler J. Kirby, Shawn A. Stasko, Honglu Liu, Esther E. Dupont-Versteegden, John J. McCarthy, Charlotte A. Peterson

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Our aim in the current study was to determine the necessity of satellite cells for long-term muscle growth and maintenance. We utilized a transgenic Pax7-DTA mouse model, allowing for the conditional depletion of > 90% of satellite cells with tamoxifen treatment. Synergist ablation surgery, where removal of synergist muscles places functional overload on the plantaris, was used to stimulate robust hypertrophy. Following 8 wk of overload, satellite celldepleted muscle demonstrated an accumulation of extracellular matrix (ECM) and fibroblast expansion that resulted in reduced specific force of the plantaris. Although the early growth response was normal, an attenuation of hypertrophy measured by both muscle wet weight and fiber cross-sectional area occurred in satellite cell-depleted muscle. Isolated primary myogenic progenitor cells (MPCs) negatively regulated fibroblast ECM mRNA expression in vitro, suggesting a novel role for activated satellite cells/MPCs in muscle adaptation. These results provide evidence that satellite cells regulate the muscle environment during growth.

Original languageEnglish (US)
Pages (from-to)1654-1665
Number of pages12
JournalFASEB Journal
Volume28
Issue number4
DOIs
StatePublished - 2014
Externally publishedYes

Fingerprint

Hypertrophy
Muscle
Satellites
Muscles
Fibers
Muscle Cells
Extracellular Matrix
Stem Cells
Skeletal Muscle Satellite Cells
Growth
Fibroblasts
Tamoxifen
Maintenance
Ablation
Differential thermal analysis
Surgery
Weights and Measures
Messenger RNA

Keywords

  • Extracellular matrix
  • Fibroblast
  • Myogenic progenitor cells
  • Pax7
  • Tcf4

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Fry, C., Lee, J. D., Jackson, J. R., Kirby, T. J., Stasko, S. A., Liu, H., ... Peterson, C. A. (2014). Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. FASEB Journal, 28(4), 1654-1665. https://doi.org/10.1096/fj.13-239426

Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. / Fry, Christopher; Lee, Jonah D.; Jackson, Janna R.; Kirby, Tyler J.; Stasko, Shawn A.; Liu, Honglu; Dupont-Versteegden, Esther E.; McCarthy, John J.; Peterson, Charlotte A.

In: FASEB Journal, Vol. 28, No. 4, 2014, p. 1654-1665.

Research output: Contribution to journalArticle

Fry, C, Lee, JD, Jackson, JR, Kirby, TJ, Stasko, SA, Liu, H, Dupont-Versteegden, EE, McCarthy, JJ & Peterson, CA 2014, 'Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy', FASEB Journal, vol. 28, no. 4, pp. 1654-1665. https://doi.org/10.1096/fj.13-239426
Fry, Christopher ; Lee, Jonah D. ; Jackson, Janna R. ; Kirby, Tyler J. ; Stasko, Shawn A. ; Liu, Honglu ; Dupont-Versteegden, Esther E. ; McCarthy, John J. ; Peterson, Charlotte A. / Regulation of the muscle fiber microenvironment by activated satellite cells during hypertrophy. In: FASEB Journal. 2014 ; Vol. 28, No. 4. pp. 1654-1665.
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