Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells

Ting Feng, Yingzi Cong, Katie Alexander, Charles O. Elson

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Toll-like receptor (TLR) 5 has been shown to maintain intestinal homeostasis and regulate host defense against enterobacterial infection. However, how TLR5 expression is regulated and its function in the intestine have not been fully elucidated. Here we demonstrate that mucosal dendritic cells (DCs), but not splenic DCs, express high levels of TLR5 protein. Alternatively spliced Tlr5 transcripts were identified but it did not explain the selective expression of TLR5 on mucosal DCs. Treatment with various bacterial ligands downregulated BMDC TLR5 expression, while retinoic acid and host stromal cell-derived signals promoted TLR5 expression in a TGF-β-independent mechanism. Signaling through TLR5 restrained regulatory T (Treg) cell generation, and accordingly, TLR5 -/- mice displayed increased frequencies of Foxp3 + Treg cells in the intestinal lamina propria. Our data indicate that bacterial and host factors differentially regulate DC TLR5 expression. TLR5 signaling regulates immune responses towards the microbiota via modulation of the Treg/effector T cell balance.

Original languageEnglish (US)
Article numbere35918
JournalPLoS One
Volume7
Issue number4
DOIs
StatePublished - Apr 24 2012

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Toll-Like Receptor 5
dendritic cells
Gene expression
Dendritic Cells
Regulatory T-Lymphocytes
Gene Expression
gene expression
Enterobacteriaceae Infections
T-lymphocytes
T-cells
stromal cells
retinoic acid
Microbiota
alternative splicing
Stromal Cells
Tretinoin
Intestines
homeostasis
Mucous Membrane
intestines

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. / Feng, Ting; Cong, Yingzi; Alexander, Katie; Elson, Charles O.

In: PLoS One, Vol. 7, No. 4, e35918, 24.04.2012.

Research output: Contribution to journalArticle

Feng, Ting ; Cong, Yingzi ; Alexander, Katie ; Elson, Charles O. / Regulation of Toll-like receptor 5 gene expression and function on mucosal dendritic cells. In: PLoS One. 2012 ; Vol. 7, No. 4.
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