Regulation of vascular tone, angiogenesis and cellular bioenergetics by the 3-mercaptopyruvate sulfurtransferase/H<inf>2</inf>S pathway: Functional impairment by hyperglycemia and restoration by dl-α-lipoic acid

Ciro Coletta, Katalin Modis, Bartosz Szczesny, Attila Brunyánszki, Gábor Oláh, Ester C S Rios, Kazunori Yanagi, Akbar Ahmad, Andreas Papapetropoulos, Csaba Szabo

Research output: Contribution to journalArticle

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Abstract

Hydrogen sulfide (H<inf>2</inf>S), as a reducing agent and an antioxidant molecule, exerts protective effects against hyperglycemic stress in the vascular endothelium. The mitochondrial enzyme 3-mercaptopyruvate sulfurtransferase (3-MST) is an important biological source of H<inf>2</inf>S. We have recently demonstrated that 3-MST activity is inhibited by oxidative stress in vitro and speculated that this may have an adverse effect on cellular homeostasis. In the current study, given the importance of H<inf>2</inf>S as a vasorelaxant, angiogenesis stimulator and cellular bioenergetic mediator, we first determined whether the 3-MST/H2S system plays a physiological regulatory role in endothelial cells. Next, we tested whether a dysfunction of this pathway develops during the development of hyperglycemia and diabetes-associated vascular complications. Intraperitoneal (IP) 3-MP (1 mg/kg) raised plasma H<inf>2</inf>S levels in rats. 3-MP (10 μmol/L to 1 mmol/L) promoted angiogenesis in vitro in bEnd3 microvascular endothelial cells and in vivo in a Matrigel assay in mice (0.3–1 mg/kg). In vitro studies with bEnd3 cell homogenates demonstrated that the 3-MP-induced increases in H<inf>2</inf>S production depended on enzymatic activity, although at higher concentrations (1–3 mmol/L) there was also evidence for an additional nonenzymatic H<inf>2</inf>S production by 3-MP. In vivo, 3-MP facilitated wound healing in rats, induced the relaxation of dermal microvessels and increased mitochondrial bioenergetic function. In vitro hyperglycemia or in vivo streptozotocin diabetes impaired angiogenesis, attenuated mitochondrial function and delayed wound healing; all of these responses were associated with an impairment of the proangiogenic and bioenergetic effects of 3-MP. The antioxidants DL-α-lipoic acid (LA) in vivo, or dihydrolipoic acid (DHLA) in vitro restored the ability of 3-MP to stimulate angiogenesis, cellular bioenergetics and wound healing in hyperglycemia and diabetes. We conclude that diabetes leads to an impairment of the 3-MST/H<inf>2</inf>S pathway, and speculate that this may contribute to the pathogenesis of hyperglycemic endothelial cell dysfunction. We also suggest that therapy with H<inf>2</inf>S donors, or treatment with the combination of 3-MP and lipoic acid may be beneficial in improving angiogenesis and bioenergetics in hyperglycemia.

Original languageEnglish (US)
Pages (from-to)1-14
Number of pages14
JournalMolecular Medicine
Volume21
DOIs
StatePublished - Feb 18 2015

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Thioctic Acid
Hyperglycemia
Energy Metabolism
Blood Vessels
Wound Healing
Endothelial Cells
Antioxidants
Hydrogen Sulfide
Experimental Diabetes Mellitus
Angiogenesis Inducing Agents
Reducing Agents
Vascular Endothelium
Microvessels
Vasodilator Agents
Oxidative Stress
Homeostasis
3-mercaptopyruvic acid
In Vitro Techniques
Skin
Enzymes

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Molecular Medicine
  • Genetics(clinical)

Cite this

Regulation of vascular tone, angiogenesis and cellular bioenergetics by the 3-mercaptopyruvate sulfurtransferase/H<inf>2</inf>S pathway : Functional impairment by hyperglycemia and restoration by dl-α-lipoic acid. / Coletta, Ciro; Modis, Katalin; Szczesny, Bartosz; Brunyánszki, Attila; Oláh, Gábor; Rios, Ester C S; Yanagi, Kazunori; Ahmad, Akbar; Papapetropoulos, Andreas; Szabo, Csaba.

In: Molecular Medicine, Vol. 21, 18.02.2015, p. 1-14.

Research output: Contribution to journalArticle

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