Regulation of vesicle transport in CV-1 cells and extracts

S. F. Hamm-Alvarez, P. Y. Kim, Michael Sheetz

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

To investigate the regulation of microtubule-dependent vesicle motility, we have studied the effects of pharmacological agents on the frequency and velocity of small vesicle movements in intact CV-1 cells. Nocodazole, but not cytochalasin B or D, abolished vesicle movements, indicating that these movements were microtubule and not actin-dependent. The frequency of vesicle movements was stimulated maximally sixfold by okadaic acid from a resting value of 1.6 movements/min per μm2 in serum-starved CV-1 cells. Other activators in decreasing order of effectiveness are fetal calf serum, dibutyryl cAMP, cholera toxin, genistein, A23187, and trental. On the other side, taxol inhibited vesicle movements by a factor of four. The activators, okadaic acid, fetal calf serum, and dibutyryl cAMP, also increased vesicle velocity and run length, while taxol decreased vesicle velocity. Although modulation of the frequency of vesicle movements over a > 20-fold range was observed, under all conditions the fraction of vesicles moving inward versus outward did not significantly change. Only in the case of taxol was the distribution of microtubules altered within this same time period. Both inward and outward microtubule-dependent vesicle movements therefore appear to be coordinately regulated. The enhanced vesicle motility elicited by fetal calf serum in intact cells correlated with in vitro measurements of vesicle motility and velocity on purified microtubules using microtubule affinity-purified motors and carbonate-washed vesicles from cells treated with fetal calf serum. This suggests that the amount of vesicular intracellular membrane traffic is coordinately regulated with microtubule-dependent motor activity.

Original languageEnglish (US)
Pages (from-to)955-966
Number of pages12
JournalJournal of Cell Science
Volume106
Issue number3
StatePublished - Dec 10 1993
Externally publishedYes

Fingerprint

Transport Vesicles
Cell Extracts
Microtubules
Paclitaxel
Okadaic Acid
Serum
Nocodazole
Cytochalasin D
Pentoxifylline
Intracellular Membranes
Cytochalasin B
Genistein
Cholera Toxin
Carbonates
Calcimycin
1,2-diamino-1,2-N,N'-carbonyl-1,2-dideoxyglucose hydrate
Actins
Motor Activity
Pharmacology

Keywords

  • Membrane traffic cAMP
  • Microtubule
  • Okadaic acid
  • Serum
  • Vesicle motility

ASJC Scopus subject areas

  • Cell Biology

Cite this

Regulation of vesicle transport in CV-1 cells and extracts. / Hamm-Alvarez, S. F.; Kim, P. Y.; Sheetz, Michael.

In: Journal of Cell Science, Vol. 106, No. 3, 10.12.1993, p. 955-966.

Research output: Contribution to journalArticle

Hamm-Alvarez, SF, Kim, PY & Sheetz, M 1993, 'Regulation of vesicle transport in CV-1 cells and extracts', Journal of Cell Science, vol. 106, no. 3, pp. 955-966.
Hamm-Alvarez, S. F. ; Kim, P. Y. ; Sheetz, Michael. / Regulation of vesicle transport in CV-1 cells and extracts. In: Journal of Cell Science. 1993 ; Vol. 106, No. 3. pp. 955-966.
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