Regulation of Wnt signaling by nociceptive input in animal models

Yuqiang Shi, Subo Yuan, Bei Li, Jigong Wang, Susan M. Carlton, Kyungsoon Chung, Jin Chung, Shao-Jun Tang

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Central sensitization-associated synaptic plasticity in the spinal cord dorsal horn (SCDH) critically contributes to the development of chronic pain, but understanding of the underlying molecular pathways is still incomplete. Emerging evidence suggests that Wnt signaling plays a crucial role in regulation of synaptic plasticity. Little is known about the potential function of the Wnt signaling cascades in chronic pain development.Results: Fluorescent immunostaining results indicate that β-catenin, an essential protein in the canonical Wnt signaling pathway, is expressed in the superficial layers of the mouse SCDH with enrichment at synapses in lamina II. In addition, Wnt3a, a prototypic Wnt ligand that activates the canonical pathway, is also enriched in the superficial layers. Immunoblotting analysis indicates that both Wnt3a a β-catenin are up-regulated in the SCDH of various mouse pain models created by hind-paw injection of capsaicin, intrathecal (i.t.) injection of HIV-gp120 protein or spinal nerve ligation (SNL). Furthermore, Wnt5a, a prototypic Wnt ligand for non-canonical pathways, and its receptor Ror2 are also up-regulated in the SCDH of these models.Conclusion: Our results suggest that Wnt signaling pathways are regulated by nociceptive input. The activation of Wnt signaling may regulate the expression of spinal central sensitization during the development of acute and chronic pain.

Original languageEnglish (US)
Article number47
JournalMolecular Pain
Volume8
DOIs
StatePublished - Jun 19 2012

Fingerprint

Wnt Signaling Pathway
Animal Models
Chronic Pain
Central Nervous System Sensitization
Catenins
Neuronal Plasticity
Substantia Gelatinosa
Ligands
Human Immunodeficiency Virus Proteins
Spinal Injections
Spinal Nerves
Capsaicin
Acute Pain
Immunoblotting
Synapses
Ligation
Pain
Injections
Spinal Cord Dorsal Horn

Keywords

  • β-catenin
  • Nociception
  • Pain
  • Spinal dorsal horn
  • Synapse
  • Wnt

ASJC Scopus subject areas

  • Anesthesiology and Pain Medicine
  • Molecular Medicine
  • Cellular and Molecular Neuroscience

Cite this

Regulation of Wnt signaling by nociceptive input in animal models. / Shi, Yuqiang; Yuan, Subo; Li, Bei; Wang, Jigong; Carlton, Susan M.; Chung, Kyungsoon; Chung, Jin; Tang, Shao-Jun.

In: Molecular Pain, Vol. 8, 47, 19.06.2012.

Research output: Contribution to journalArticle

Shi Y, Yuan S, Li B, Wang J, Carlton SM, Chung K et al. Regulation of Wnt signaling by nociceptive input in animal models. Molecular Pain. 2012 Jun 19;8. 47. https://doi.org/10.1186/1744-8069-8-47
Shi, Yuqiang ; Yuan, Subo ; Li, Bei ; Wang, Jigong ; Carlton, Susan M. ; Chung, Kyungsoon ; Chung, Jin ; Tang, Shao-Jun. / Regulation of Wnt signaling by nociceptive input in animal models. In: Molecular Pain. 2012 ; Vol. 8.
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