Reinfection in American Cutaneous Leishmaniasis

Evaluation of Clinical Outcomes in the Hamster Model

Y. Osorio, S. J. Gonzalez, V. L. Gama, B. L. Travi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. panamensis) species of leishmaniae were conducted in the hamster model. The different protocols for primary infections prior to the challenge with L. panamensis were as follows: (a) L. major, single promastigote injection, (b) L. major, three booster infections, (c) L. panamensis, followed by antimonial treatment to achieve subclinical infection, (d) L. panamensis, with active lesions, (e) sham infected, naive controls. Although all reinfected hamsters developed lesions upon challenge, animals with active primary lesions due to L. panamensis, and receiving booster infections of L. major had the most benign secondary lesions (58-91% and 69-76% smaller than controls, respectively, P<0.05). Subclinically infected animals had intermediate lesions (40-64% smaller than controls, P<0.05), while hamsters which received a single dose of L. major had no significant improvement over controls. Our results suggested that L. major could elicit a cross protective response to L. panamensis, and that the presence and number of amastigotes persisting after a primary infection may influence the clinical outcome of reinfections.

Original languageEnglish
Pages (from-to)353-356
Number of pages4
JournalMemorias do Instituto Oswaldo Cruz
Volume93
Issue number3
StatePublished - May 1998
Externally publishedYes

Fingerprint

Cutaneous Leishmaniasis
Cricetinae
Infection
Animals
Asymptomatic Infections
Leishmania
Injections
Experiments
Therapeutics

Keywords

  • Hamster
  • Immunoprophylaxis
  • Leishmania major
  • Leishmania panamensis
  • Reinfection

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Immunology
  • Virology
  • Infectious Diseases
  • Microbiology (medical)

Cite this

Reinfection in American Cutaneous Leishmaniasis : Evaluation of Clinical Outcomes in the Hamster Model. / Osorio, Y.; Gonzalez, S. J.; Gama, V. L.; Travi, B. L.

In: Memorias do Instituto Oswaldo Cruz, Vol. 93, No. 3, 05.1998, p. 353-356.

Research output: Contribution to journalArticle

@article{0646344c52ab434dbcf460e8f57835a8,
title = "Reinfection in American Cutaneous Leishmaniasis: Evaluation of Clinical Outcomes in the Hamster Model",
abstract = "There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. panamensis) species of leishmaniae were conducted in the hamster model. The different protocols for primary infections prior to the challenge with L. panamensis were as follows: (a) L. major, single promastigote injection, (b) L. major, three booster infections, (c) L. panamensis, followed by antimonial treatment to achieve subclinical infection, (d) L. panamensis, with active lesions, (e) sham infected, naive controls. Although all reinfected hamsters developed lesions upon challenge, animals with active primary lesions due to L. panamensis, and receiving booster infections of L. major had the most benign secondary lesions (58-91{\%} and 69-76{\%} smaller than controls, respectively, P<0.05). Subclinically infected animals had intermediate lesions (40-64{\%} smaller than controls, P<0.05), while hamsters which received a single dose of L. major had no significant improvement over controls. Our results suggested that L. major could elicit a cross protective response to L. panamensis, and that the presence and number of amastigotes persisting after a primary infection may influence the clinical outcome of reinfections.",
keywords = "Hamster, Immunoprophylaxis, Leishmania major, Leishmania panamensis, Reinfection",
author = "Y. Osorio and Gonzalez, {S. J.} and Gama, {V. L.} and Travi, {B. L.}",
year = "1998",
month = "5",
language = "English",
volume = "93",
pages = "353--356",
journal = "Memorias do Instituto Oswaldo Cruz",
issn = "0074-0276",
publisher = "Fundacao Oswaldo Cruz",
number = "3",

}

TY - JOUR

T1 - Reinfection in American Cutaneous Leishmaniasis

T2 - Evaluation of Clinical Outcomes in the Hamster Model

AU - Osorio, Y.

AU - Gonzalez, S. J.

AU - Gama, V. L.

AU - Travi, B. L.

PY - 1998/5

Y1 - 1998/5

N2 - There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. panamensis) species of leishmaniae were conducted in the hamster model. The different protocols for primary infections prior to the challenge with L. panamensis were as follows: (a) L. major, single promastigote injection, (b) L. major, three booster infections, (c) L. panamensis, followed by antimonial treatment to achieve subclinical infection, (d) L. panamensis, with active lesions, (e) sham infected, naive controls. Although all reinfected hamsters developed lesions upon challenge, animals with active primary lesions due to L. panamensis, and receiving booster infections of L. major had the most benign secondary lesions (58-91% and 69-76% smaller than controls, respectively, P<0.05). Subclinically infected animals had intermediate lesions (40-64% smaller than controls, P<0.05), while hamsters which received a single dose of L. major had no significant improvement over controls. Our results suggested that L. major could elicit a cross protective response to L. panamensis, and that the presence and number of amastigotes persisting after a primary infection may influence the clinical outcome of reinfections.

AB - There is no clear understanding of the outcome of reinfection in New World cutaneous leishmaniasis, and its role in the relationship to the development of protection or secondary disease. For this reason, reinfection experiments with homologous (Leishmania panamensis-L. panamensis) and heterologous (L. major-L. panamensis) species of leishmaniae were conducted in the hamster model. The different protocols for primary infections prior to the challenge with L. panamensis were as follows: (a) L. major, single promastigote injection, (b) L. major, three booster infections, (c) L. panamensis, followed by antimonial treatment to achieve subclinical infection, (d) L. panamensis, with active lesions, (e) sham infected, naive controls. Although all reinfected hamsters developed lesions upon challenge, animals with active primary lesions due to L. panamensis, and receiving booster infections of L. major had the most benign secondary lesions (58-91% and 69-76% smaller than controls, respectively, P<0.05). Subclinically infected animals had intermediate lesions (40-64% smaller than controls, P<0.05), while hamsters which received a single dose of L. major had no significant improvement over controls. Our results suggested that L. major could elicit a cross protective response to L. panamensis, and that the presence and number of amastigotes persisting after a primary infection may influence the clinical outcome of reinfections.

KW - Hamster

KW - Immunoprophylaxis

KW - Leishmania major

KW - Leishmania panamensis

KW - Reinfection

UR - http://www.scopus.com/inward/record.url?scp=0032065254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032065254&partnerID=8YFLogxK

M3 - Article

VL - 93

SP - 353

EP - 356

JO - Memorias do Instituto Oswaldo Cruz

JF - Memorias do Instituto Oswaldo Cruz

SN - 0074-0276

IS - 3

ER -