Relationship between extent of burn injury and magnitude of microbial translocation from the intestine

L. Gianotti, J. W. Alexander, Tonyia Eaves-Pyles, L. James, G. F. Babcock

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0%, 10%, 20%, 30%, or 50% total body surface area full-thickness burn and simultaneous gavage with 1 x 1010 14C-labeled Escherichia coli. Mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30%. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.

Original languageEnglish (US)
Pages (from-to)336-342
Number of pages7
JournalJournal of Burn Care and Rehabilitation
Volume14
Issue number3
StatePublished - 1993
Externally publishedYes

Fingerprint

Intestines
Wounds and Injuries
Lymph Nodes
Escherichia coli
Bacterial Translocation
Bacterial Load
Ascitic Fluid
Body Surface Area
Liver
Enterobacteriaceae
Radioactivity
Sepsis
Spleen
Hot Temperature
Bacteria
Skin

ASJC Scopus subject areas

  • Emergency Medicine
  • Rehabilitation
  • Surgery
  • Nursing(all)
  • Health Professions(all)

Cite this

Relationship between extent of burn injury and magnitude of microbial translocation from the intestine. / Gianotti, L.; Alexander, J. W.; Eaves-Pyles, Tonyia; James, L.; Babcock, G. F.

In: Journal of Burn Care and Rehabilitation, Vol. 14, No. 3, 1993, p. 336-342.

Research output: Contribution to journalArticle

@article{d62e0cfa8e294cfcad10a40e87c7ef9d,
title = "Relationship between extent of burn injury and magnitude of microbial translocation from the intestine",
abstract = "The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0{\%}, 10{\%}, 20{\%}, 30{\%}, or 50{\%} total body surface area full-thickness burn and simultaneous gavage with 1 x 1010 14C-labeled Escherichia coli. Mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30{\%}. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.",
author = "L. Gianotti and Alexander, {J. W.} and Tonyia Eaves-Pyles and L. James and Babcock, {G. F.}",
year = "1993",
language = "English (US)",
volume = "14",
pages = "336--342",
journal = "Journal of Burn Care and Research",
issn = "1559-047X",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Relationship between extent of burn injury and magnitude of microbial translocation from the intestine

AU - Gianotti, L.

AU - Alexander, J. W.

AU - Eaves-Pyles, Tonyia

AU - James, L.

AU - Babcock, G. F.

PY - 1993

Y1 - 1993

N2 - The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0%, 10%, 20%, 30%, or 50% total body surface area full-thickness burn and simultaneous gavage with 1 x 1010 14C-labeled Escherichia coli. Mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30%. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.

AB - The gut can be a source of sepsis after thermal injury. In the present study the relationship between the extent of burn injury and magnitude of bacterial translocation was investigated. Mice underwent 0%, 10%, 20%, 30%, or 50% total body surface area full-thickness burn and simultaneous gavage with 1 x 1010 14C-labeled Escherichia coli. Mesenteric lymph nodes, liver, spleen, peritoneal fluid, and burn wound were excised 4 hours after burn injury. Residual radioactivity and bacterial colony counts were measured, and percentages of viable organisms were calculated. Results showed that the rate of translocation of 14C E. coli increased proportionally with the burn size, reaching a maximum at 30%. The cutaneous eschar collected a remarkable amount of labeled bacteria, suggesting enteric microflora as a possible source of contamination of the burn wound via endogenous routes. The percentage of viable organisms in the tissues demonstrated that the ability of mesenteric lymph nodes, liver, and eschar to clear translocated bacteria was directly affected by the severity of the burn injury.

UR - http://www.scopus.com/inward/record.url?scp=0027318855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027318855&partnerID=8YFLogxK

M3 - Article

VL - 14

SP - 336

EP - 342

JO - Journal of Burn Care and Research

JF - Journal of Burn Care and Research

SN - 1559-047X

IS - 3

ER -