Relationship between non-enzymatic glycosylation and changes in serum insulin like growth factor-1 (IGF-1) and IGF binding protein-3 levels in patients with type 2 diabetes mellitus

A. M. Cortizo, Phillip Lee, N. V. Cédola, H. Jasper, J. J. Gagliardino

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18 Citations (Scopus)

Abstract

The possible occurrence of increased non-enzymatic glycosylation of serum insulin-like growth factor binding protein-3 (IGFBP-3) in vivo and the changes that would simultaneously occur in serum levels of IGFBP-3 and insulin-like growth factor-1 (IGF-I) were investigated. We measured levels of IGF-I and IGFBP-3 and the degree of glycation of total serum protein and IGFBP-3, in serum samples obtained from patients with poorly controlled noninsulin-dependent diabetes (type 2) and from age-matched non-diabetic controls. Type 2 diabetic patients had significantly higher glycated serum protein (GlyP) levels. GlyP significantly correlated with age in the control (r = 0.315, P < 0.05) but not in the type 2 diabetes group. Control and diabetic subjects had comparable serum IGF-I levels and in both groups IGF-I levels tended to decrease with age (r = -0.567, P < 0.001 and r = -0.465, P < 0.05 for control and type 2 diabetic subjects, respectively). In the type 2 diabetes group, IGF-I levels showed a negative correlation with serum GlyP values (r = -0.476, P < 0.05). Type 2 diabetic and control patients had comparable serum IGFBP-3 levels, which were significantly higher in diabetic patients in the older age subgroups. A negative correlation was found between IGFBP-3 levels and age in the control (r = -0.705, P < 0.001) and in the type 2 diabetes groups (r = -0.463, P < 0.05. A significant negative correlation was found between IGFBP-3 levels and GlyP in control (r = -0.449, P < 0.002) but not in type 2 diabetic subjects. The mean glycated IGFBP-3 (GlyIGFBP-3) levels were higher in the oldest type 2 diabetic patients. In these patients, GlyIGFBP-3 was negatively associated with IGF-I levels (r = -0.447, P < 0.05). The IGF-I/IGFBP-3 molar ratio was significantly reduced in the 46-60-year-old type 2 diabetic group, whereas the IGF-I/IGFBP-3 ratio was positively and significantly correlated with GlyP levels only in the control group (r = 0.489, P < 0.01). Our results show that: a) increased non-enzymatic glycosylation of IGFBP-3 occurs in vivo; and b) this effect is accompanied by an increase in IGFBP-3 levels. These results suggest that the IGF-I/IGFBP-3 system is another target for the metabolic derangements of type 2 diabetes. Its alterations might play a role in diabetic complications.

Original languageEnglish (US)
Pages (from-to)85-90
Number of pages6
JournalActa Diabetologica
Volume35
Issue number2
DOIs
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

Insulin-Like Growth Factor Binding Protein 3
Somatomedins
Glycosylation
Type 2 Diabetes Mellitus
Insulin-Like Growth Factor I
Serum
Blood Proteins
Diabetes Complications

Keywords

  • Binding proteins
  • Chronic complications
  • Diabetes mellitus
  • Insulin-like growth factor
  • Non-enzymatic glycosylation

ASJC Scopus subject areas

  • Endocrinology
  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism

Cite this

Relationship between non-enzymatic glycosylation and changes in serum insulin like growth factor-1 (IGF-1) and IGF binding protein-3 levels in patients with type 2 diabetes mellitus. / Cortizo, A. M.; Lee, Phillip; Cédola, N. V.; Jasper, H.; Gagliardino, J. J.

In: Acta Diabetologica, Vol. 35, No. 2, 07.1998, p. 85-90.

Research output: Contribution to journalArticle

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AU - Gagliardino, J. J.

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KW - Chronic complications

KW - Diabetes mellitus

KW - Insulin-like growth factor

KW - Non-enzymatic glycosylation

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