Relaxation kinetics of rat aorta during pregnancy

Venu Jain, Yuri P. Vedernikov, George Saade, Kristof Chwalisz, Robert E. Garfield

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE: To examine the hypothesis that kinetics of vasorelaxation are altered during pregnancy. METHODS: Rings of aorta from rats at different stages of pregnancy (early, late, and term) and from nonpregnant female rats were precontracted with high-K+ and then allowed to relax in normal-K+ physiologic saline solution. The time to reach 50% and 80% relaxation were determined in the absence or presence of a cyclooxygenase inhibitor (indomethacin) and a nitric oxide synthase inhibitor (N(Ω)-nitro-L-arginine methyl ester) or after removal of the endothelium. RESULTS: The aortic relaxation was progressively faster in later stages of gestation. N(Ω)- nitro-L-arginine methyl ester and indomethacin had no significant effect whereas removal of the endothelium caused a slowing of relaxation in all the groups. Even in the presence of N(Ω)-nitro-L-arginine methyl ester and indomethacin or after de-endothelization, the relaxation remained faster at term as compared with the other groups. CONCLUSION: Aortic relaxation is faster in the presence of endothelium. The effect of endothelium on relaxation is independent of nitric oxide synthase or cyclooxygenase systems. Progression of gestation is associated with acceleration of aortic relaxation, which cannot be totally ascribed to an endothelial factor and may involve a change intrinsic to the vascular smooth muscle. Faster relaxation kinetics of the vasculature during pregnancy may be a mechanism to decrease peripheral vascular resistance.

Original languageEnglish
Pages (from-to)81-86
Number of pages6
JournalJournal of the Society for Gynecologic Investigation
Volume5
Issue number2
DOIs
StatePublished - Mar 1998
Externally publishedYes

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Aorta
Endothelium
Pregnancy
Indomethacin
Nitric Oxide Synthase
Vascular Resistance
Cyclooxygenase Inhibitors
Prostaglandin-Endoperoxide Synthases
Vascular Smooth Muscle
Vasodilation
Sodium Chloride
arginine methyl ester

Keywords

  • Endothelium
  • Pregnancy
  • Rat aorta
  • Relaxation kinetics
  • Vascular smooth muscle

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Relaxation kinetics of rat aorta during pregnancy. / Jain, Venu; Vedernikov, Yuri P.; Saade, George; Chwalisz, Kristof; Garfield, Robert E.

In: Journal of the Society for Gynecologic Investigation, Vol. 5, No. 2, 03.1998, p. 81-86.

Research output: Contribution to journalArticle

Jain, Venu ; Vedernikov, Yuri P. ; Saade, George ; Chwalisz, Kristof ; Garfield, Robert E. / Relaxation kinetics of rat aorta during pregnancy. In: Journal of the Society for Gynecologic Investigation. 1998 ; Vol. 5, No. 2. pp. 81-86.
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