Release of AMP and adenosine from rat heart mitochondria

Gregory K. Asimakis, Douglas E. Wilson, Vincent Conti

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extramitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90% and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.

Original languageEnglish (US)
Pages (from-to)2373-2380
Number of pages8
JournalLife Sciences
Volume37
Issue number25
DOIs
StatePublished - Dec 23 1985

Fingerprint

Heart Mitochondria
Mitochondria
Adenosine Monophosphate
Adenosine
Rats
Phosphates
Rotenone
Adenine Nucleotides
Glutamic Acid
Substrates

ASJC Scopus subject areas

  • Pharmacology

Cite this

Release of AMP and adenosine from rat heart mitochondria. / Asimakis, Gregory K.; Wilson, Douglas E.; Conti, Vincent.

In: Life Sciences, Vol. 37, No. 25, 23.12.1985, p. 2373-2380.

Research output: Contribution to journalArticle

Asimakis, Gregory K. ; Wilson, Douglas E. ; Conti, Vincent. / Release of AMP and adenosine from rat heart mitochondria. In: Life Sciences. 1985 ; Vol. 37, No. 25. pp. 2373-2380.
@article{24e5e9cecd404fdca475b606539fdd7f,
title = "Release of AMP and adenosine from rat heart mitochondria",
abstract = "Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extramitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90{\%} and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.",
author = "Asimakis, {Gregory K.} and Wilson, {Douglas E.} and Vincent Conti",
year = "1985",
month = "12",
day = "23",
doi = "10.1016/0024-3205(85)90104-3",
language = "English (US)",
volume = "37",
pages = "2373--2380",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "25",

}

TY - JOUR

T1 - Release of AMP and adenosine from rat heart mitochondria

AU - Asimakis, Gregory K.

AU - Wilson, Douglas E.

AU - Conti, Vincent

PY - 1985/12/23

Y1 - 1985/12/23

N2 - Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extramitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90% and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.

AB - Release of AMP and adenosine from rat heart mitochondria was studied. The rate of appearance of extramitochondrial adenosine was independent of the extramitochondrial phosphate concentration between 5 and 20 mM. In the absence of exogenous, respiratory substrates or in the presence of glutamate/malate plus rotenone, the rate of appearance of adenosine was relatively low when phosphate was not added. The appearance of extramitochondrial AMP + adenosine was found to be directly proportional to the extramitochondrial phosphate concentration. Zn2+ (10 mM) decreased the rate of adenosine appearance by 90% and increased the rate of AMP appearance 6-fold. The mitochondrial preparations dephosphorylated exogenous AMP; this activity was inhibited by 10 mM Zn2+. We conclude that the adenosine appearing in the extramitochondrial space was not due to a direct release from the matrix, but instead was due to adenine nucleotide release with subsequent conversion to adenosine in the extramitochondrial space.

UR - http://www.scopus.com/inward/record.url?scp=0022349747&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022349747&partnerID=8YFLogxK

U2 - 10.1016/0024-3205(85)90104-3

DO - 10.1016/0024-3205(85)90104-3

M3 - Article

C2 - 2867447

AN - SCOPUS:0022349747

VL - 37

SP - 2373

EP - 2380

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 25

ER -