Release of slow reacting substance of anaphylaxis from human leukocytes

J. A. Grant, L. M. Lichtenstein

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Mediators of immediate hypersensitivity were released from human leukocytes by means of allergen challenge. Leukocytes from 12 consecutive donors were challenged with allergens to which these donors were clinically sensitive. In all, histamine and a second substance (SRS A(leuk)) which caused prolonged contraction of guinea pig ileum, were released. No SRS A(leuk) was obtained without allergen challenge, and none was observed from nonatopic leukocytes challenged with the same allergens. The time of release after addition of allergens and the allergen dose response curves for release of both histamine and SRS A(leuk) were quite similar. SRS A(leuk) was synthesized de novo after allergen challenge since this factor was not demonstrated in disrupted cells. SRS A(leuk) was soluble in 80% ethanol and was not destroyed by base hydrolysis. The smooth muscle contracting activity of SRS A(leuk) resembled that of slow reacting substance of anaphylaxis (SRS A) obtained from human lung and rat peritoneal cells, but was different from histamine, bradykinin, serotonin, and prostaglandin E1 and F(2α). Preliminary studies suggest that both histamine and SRS A(leuk) are synthesized by human basophils. It is concluded that SRS A is generated by human leukocytes as well as human lung and nasal polyps, and suggest that this mediator may play an important role in the pathogenesis of human immediate hypersensitivity reactions.

Original languageEnglish (US)
Pages (from-to)897-904
Number of pages8
JournalJournal of Immunology
Volume112
Issue number3
StatePublished - 1974

Fingerprint

SRS-A
Leukocytes
Allergens
Histamine
Immediate Hypersensitivity
Nasal Polyps
Lung
Basophils
Alprostadil
Histamine Release
Prostaglandins F
Bradykinin
Ileum
Smooth Muscle
Serotonin
Guinea Pigs
Hydrolysis
Ethanol

ASJC Scopus subject areas

  • Immunology

Cite this

Grant, J. A., & Lichtenstein, L. M. (1974). Release of slow reacting substance of anaphylaxis from human leukocytes. Journal of Immunology, 112(3), 897-904.

Release of slow reacting substance of anaphylaxis from human leukocytes. / Grant, J. A.; Lichtenstein, L. M.

In: Journal of Immunology, Vol. 112, No. 3, 1974, p. 897-904.

Research output: Contribution to journalArticle

Grant, JA & Lichtenstein, LM 1974, 'Release of slow reacting substance of anaphylaxis from human leukocytes', Journal of Immunology, vol. 112, no. 3, pp. 897-904.
Grant, J. A. ; Lichtenstein, L. M. / Release of slow reacting substance of anaphylaxis from human leukocytes. In: Journal of Immunology. 1974 ; Vol. 112, No. 3. pp. 897-904.
@article{bf614ebcdd2e46fea7be966017f246e9,
title = "Release of slow reacting substance of anaphylaxis from human leukocytes",
abstract = "Mediators of immediate hypersensitivity were released from human leukocytes by means of allergen challenge. Leukocytes from 12 consecutive donors were challenged with allergens to which these donors were clinically sensitive. In all, histamine and a second substance (SRS A(leuk)) which caused prolonged contraction of guinea pig ileum, were released. No SRS A(leuk) was obtained without allergen challenge, and none was observed from nonatopic leukocytes challenged with the same allergens. The time of release after addition of allergens and the allergen dose response curves for release of both histamine and SRS A(leuk) were quite similar. SRS A(leuk) was synthesized de novo after allergen challenge since this factor was not demonstrated in disrupted cells. SRS A(leuk) was soluble in 80{\%} ethanol and was not destroyed by base hydrolysis. The smooth muscle contracting activity of SRS A(leuk) resembled that of slow reacting substance of anaphylaxis (SRS A) obtained from human lung and rat peritoneal cells, but was different from histamine, bradykinin, serotonin, and prostaglandin E1 and F(2α). Preliminary studies suggest that both histamine and SRS A(leuk) are synthesized by human basophils. It is concluded that SRS A is generated by human leukocytes as well as human lung and nasal polyps, and suggest that this mediator may play an important role in the pathogenesis of human immediate hypersensitivity reactions.",
author = "Grant, {J. A.} and Lichtenstein, {L. M.}",
year = "1974",
language = "English (US)",
volume = "112",
pages = "897--904",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "3",

}

TY - JOUR

T1 - Release of slow reacting substance of anaphylaxis from human leukocytes

AU - Grant, J. A.

AU - Lichtenstein, L. M.

PY - 1974

Y1 - 1974

N2 - Mediators of immediate hypersensitivity were released from human leukocytes by means of allergen challenge. Leukocytes from 12 consecutive donors were challenged with allergens to which these donors were clinically sensitive. In all, histamine and a second substance (SRS A(leuk)) which caused prolonged contraction of guinea pig ileum, were released. No SRS A(leuk) was obtained without allergen challenge, and none was observed from nonatopic leukocytes challenged with the same allergens. The time of release after addition of allergens and the allergen dose response curves for release of both histamine and SRS A(leuk) were quite similar. SRS A(leuk) was synthesized de novo after allergen challenge since this factor was not demonstrated in disrupted cells. SRS A(leuk) was soluble in 80% ethanol and was not destroyed by base hydrolysis. The smooth muscle contracting activity of SRS A(leuk) resembled that of slow reacting substance of anaphylaxis (SRS A) obtained from human lung and rat peritoneal cells, but was different from histamine, bradykinin, serotonin, and prostaglandin E1 and F(2α). Preliminary studies suggest that both histamine and SRS A(leuk) are synthesized by human basophils. It is concluded that SRS A is generated by human leukocytes as well as human lung and nasal polyps, and suggest that this mediator may play an important role in the pathogenesis of human immediate hypersensitivity reactions.

AB - Mediators of immediate hypersensitivity were released from human leukocytes by means of allergen challenge. Leukocytes from 12 consecutive donors were challenged with allergens to which these donors were clinically sensitive. In all, histamine and a second substance (SRS A(leuk)) which caused prolonged contraction of guinea pig ileum, were released. No SRS A(leuk) was obtained without allergen challenge, and none was observed from nonatopic leukocytes challenged with the same allergens. The time of release after addition of allergens and the allergen dose response curves for release of both histamine and SRS A(leuk) were quite similar. SRS A(leuk) was synthesized de novo after allergen challenge since this factor was not demonstrated in disrupted cells. SRS A(leuk) was soluble in 80% ethanol and was not destroyed by base hydrolysis. The smooth muscle contracting activity of SRS A(leuk) resembled that of slow reacting substance of anaphylaxis (SRS A) obtained from human lung and rat peritoneal cells, but was different from histamine, bradykinin, serotonin, and prostaglandin E1 and F(2α). Preliminary studies suggest that both histamine and SRS A(leuk) are synthesized by human basophils. It is concluded that SRS A is generated by human leukocytes as well as human lung and nasal polyps, and suggest that this mediator may play an important role in the pathogenesis of human immediate hypersensitivity reactions.

UR - http://www.scopus.com/inward/record.url?scp=0015955753&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015955753&partnerID=8YFLogxK

M3 - Article

VL - 112

SP - 897

EP - 904

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 3

ER -