Release of the chemokines RANTES and IL-8 by primary human corneal epithelial cells

C. K. Hallberg, K. S. Gill, J. Yannariello-Brown, H. Haeberle, P. B. Ernst, Roberto Garofalo, S. D. Trocme

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

There is mounting evidence to suggest that chemokines play an important role in the recruitment of eosinophils during an allergic response. The objective of the present study was to investigate whether primary human corneal epithelial cells (HCEs) release the chemokines 'regulated upon activation in normal T cell expressed and secreted' (RANTES) and interleukin- 8 (IL-8) after stimulation with phorbol 12-myristate 13-acetate (PMA) or selected proinflammatory and immunomodulating cytokines. Confluent primary HCE cultures were treated with various concentrations of either PMA, or the cytokines interleukin-1α (IL-1α), tumor necrosis factor-α (TNF-α), or interferon-γ (IFN-γ) in serum-free media. Culture supernatants were then collected at timed intervals, and assayed for the production of RANTES and IL-8 protein using specific enzyme-linked immunosorbent assays. Our data showed that HCEs release the chemokines RANTES and IL-8 in a time- and dose- dependent manner in response to PMA and the cytokines IL-1α and TNF-α. IFN- γ did not significantly induce the release of either RANTES or IL-8. However, a 1:1 mixture of TNF-α and IFN-γ acted synergistically in the augmentation of RANTES release. The results of the present study suggest an important role for these cytokines in the perpetuation of ocular inflammatory reactions.

Original languageEnglish (US)
Pages (from-to)67-82
Number of pages16
JournalResearch Communications in Pharmacology and Toxicology
Volume4
Issue number1-2
StatePublished - 1999

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T-cells
Interleukin-8
Chemokines
Epithelial Cells
Chemical activation
T-Lymphocytes
Interferons
Cytokines
Acetates
Tumor Necrosis Factor-alpha
Interleukin-1
Immunosorbents
Serum-Free Culture Media
Mountings
Cell culture
Eosinophils
Assays
Cell Culture Techniques
Enzyme-Linked Immunosorbent Assay
Enzymes

ASJC Scopus subject areas

  • Pharmacology
  • Toxicology

Cite this

Hallberg, C. K., Gill, K. S., Yannariello-Brown, J., Haeberle, H., Ernst, P. B., Garofalo, R., & Trocme, S. D. (1999). Release of the chemokines RANTES and IL-8 by primary human corneal epithelial cells. Research Communications in Pharmacology and Toxicology, 4(1-2), 67-82.

Release of the chemokines RANTES and IL-8 by primary human corneal epithelial cells. / Hallberg, C. K.; Gill, K. S.; Yannariello-Brown, J.; Haeberle, H.; Ernst, P. B.; Garofalo, Roberto; Trocme, S. D.

In: Research Communications in Pharmacology and Toxicology, Vol. 4, No. 1-2, 1999, p. 67-82.

Research output: Contribution to journalArticle

Hallberg, CK, Gill, KS, Yannariello-Brown, J, Haeberle, H, Ernst, PB, Garofalo, R & Trocme, SD 1999, 'Release of the chemokines RANTES and IL-8 by primary human corneal epithelial cells', Research Communications in Pharmacology and Toxicology, vol. 4, no. 1-2, pp. 67-82.
Hallberg, C. K. ; Gill, K. S. ; Yannariello-Brown, J. ; Haeberle, H. ; Ernst, P. B. ; Garofalo, Roberto ; Trocme, S. D. / Release of the chemokines RANTES and IL-8 by primary human corneal epithelial cells. In: Research Communications in Pharmacology and Toxicology. 1999 ; Vol. 4, No. 1-2. pp. 67-82.
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