Remodeling of the epithelial-connective tissue interface in oral epithelial dysplasia as visualized by noninvasive 3D imaging

Rahul Pal, Tuya Shilagard, Jinping Yang, Paula Villarreal, Tyra Brown, Suimin Qiu, Susan McCammon, Vicente Resto, Gracie Vargas

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Early neoplastic features in oral epithelial dysplasia are first evident at the basal epithelium positioned at the epithelial- connective tissue interface (ECTI), separating the basal epithelium from the underlying lamina propria. The ECTI undergoes significant deformation in early neoplasia due to focal epithelial expansion and proteolytic remodeling of the lamina propria, but few studies have examined these changes. In the present study, we quantitated alterations in ECTI topography in dysplasia using in vivo volumetric multiphoton autofluorescence microscopy and second harmonic generation microscopy. The label-free method allows direct noninvasive visualization of the ECTI surface without perturbing the epithelium. An image-based parameter, "ECTI contour, " is described that indicates deformation of the ECTI surface. ECTI contour was higher in dysplasia than control or inflamed specimens, indicating transition from flat to a deformed surface. Cellular parameters of nuclear area, nuclear density, coefficient of variation in nuclear area in the basal epithelium and collagen density in areas adjacent to ECTI were measured. ECTI contour differentiated dysplasia from control/benign mucosa with higher sensitivity and specificity than basal nuclear density or basal nuclear area, comparable with coefficient of variation in nuclear area and collagen density. The presented method offers a unique opportunity to study ECTI in intact mucosa with simultaneous assessment of cellular and extracellular matrix features, expanding opportunities for studies of early neoplastic events near this critical interface and potentially leading to development of new approaches for detecting neoplasia in vivo. Cancer Res; 76(16); 4637-47.

Original languageEnglish (US)
Pages (from-to)4637-4647
Number of pages11
JournalCancer Research
Volume76
Issue number16
DOIs
StatePublished - Aug 15 2016

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Connective Tissue
Epithelium
Mucous Membrane
Microscopy
Collagen
Neoplasms
Extracellular Matrix

ASJC Scopus subject areas

  • Medicine(all)
  • Oncology
  • Cancer Research

Cite this

Remodeling of the epithelial-connective tissue interface in oral epithelial dysplasia as visualized by noninvasive 3D imaging. / Pal, Rahul; Shilagard, Tuya; Yang, Jinping; Villarreal, Paula; Brown, Tyra; Qiu, Suimin; McCammon, Susan; Resto, Vicente; Vargas, Gracie.

In: Cancer Research, Vol. 76, No. 16, 15.08.2016, p. 4637-4647.

Research output: Contribution to journalArticle

Pal, Rahul ; Shilagard, Tuya ; Yang, Jinping ; Villarreal, Paula ; Brown, Tyra ; Qiu, Suimin ; McCammon, Susan ; Resto, Vicente ; Vargas, Gracie. / Remodeling of the epithelial-connective tissue interface in oral epithelial dysplasia as visualized by noninvasive 3D imaging. In: Cancer Research. 2016 ; Vol. 76, No. 16. pp. 4637-4647.
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