We demonstrated the feasibility of Raman spectroscopy for, remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm -1 were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14-34°C. The ratio between the integrated Raman intensities of two temperature dependent OHvibrational regions (OH2/OH1) in the spectra of water showed high linear dependence on temperature both in pure water [0.0049 (± 1.2%) T+0.4522(±0.5%), R2 = 0.99, n = 50, p<0.05], as well as in the rabbit aqueous humor [0.0036(±2.8%)T+0. 4966(±0.6%), R2 = 0.98, n=162, p<0.05] with a high degree of reproducibility and sensitivity (∼0.2-0.7°C). Raman spectroscopy can be used for high resolution and remote monitoring of temperature in the aqueous humor under in vivo conditions.
- Ocular tissue
- Raman spectroscopy
- Remote sensing
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Atomic and Molecular Physics, and Optics
- Biomedical Engineering