Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy

Noel J C Bauer, Massoud Motamedi, Fred Hendrikse, James P. Wicksted

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

We demonstrated the feasibility of Raman spectroscopy for, remote temperature monitoring within the aqueous humor of the rabbit eye in vivo. Using a confocal Raman spectroscopy system, Raman spectra from 2580 to 3800 cm -1 were recorded in HPLC-grade water and in the aqueous humor of the rabbit eye under in vivo and ex vivo conditions within a temperature range of 14-34°C. The ratio between the integrated Raman intensities of two temperature dependent OHvibrational regions (OH2/OH1) in the spectra of water showed high linear dependence on temperature both in pure water [0.0049 (± 1.2%) T+0.4522(±0.5%), R2 = 0.99, n = 50, p<0.05], as well as in the rabbit aqueous humor [0.0036(±2.8%)T+0. 4966(±0.6%), R2 = 0.98, n=162, p<0.05] with a high degree of reproducibility and sensitivity (∼0.2-0.7°C). Raman spectroscopy can be used for high resolution and remote monitoring of temperature in the aqueous humor under in vivo conditions.

Original languageEnglish (US)
Article number031109
JournalJournal of Biomedical Optics
Volume10
Issue number3
DOIs
StatePublished - May 2005

Fingerprint

Raman Spectrum Analysis
Aqueous Humor
Raman spectroscopy
Tissue
rabbits
Temperature
Monitoring
Rabbits
Water
temperature
water
Raman scattering
grade
High Pressure Liquid Chromatography
Raman spectra
sensitivity
high resolution

Keywords

  • Ocular tissue
  • Raman spectroscopy
  • Remote sensing
  • Temperature

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Clinical Biochemistry

Cite this

Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy. / Bauer, Noel J C; Motamedi, Massoud; Hendrikse, Fred; Wicksted, James P.

In: Journal of Biomedical Optics, Vol. 10, No. 3, 031109, 05.2005.

Research output: Contribution to journalArticle

Bauer, Noel J C ; Motamedi, Massoud ; Hendrikse, Fred ; Wicksted, James P. / Remote temperature monitoring in ocular tissue using confocal Raman spectroscopy. In: Journal of Biomedical Optics. 2005 ; Vol. 10, No. 3.
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